Candida konsanensis sp. nov., a new yeast species isolated from Jasminum adenophyllum in Thailand with potentially carboxymethyl cellulase-producing capability

World Journal of Microbiology and Biotechnology - Tập 29 - Trang 1481-1486 - 2013
Somporn Sarawan1, Polson Mahakhan2, Sasitorn Jindamorakot3, Kanit Vichitphan4,5, Sukanda Vichitphan4,5, Jutaporn Sawaengkaew2
1Graduate School, Khon Kaen University, Khon Kaen, Thailand
2Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand
3BIOTEC Culture Collection, National Center for Genetic Engineering and Biotechnology (BIOTEC), Klong Luang, Thailand
4Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, Thailand
5Fermentation Research Center for Value Added Agricultural Products (FerVAAP), Khon Kaen University, Khon Kaen, Thailand

Tóm tắt

A new yeast species (KKU-FW10) belonging to the Candida genus was isolated from Jasminum adenophyllum in the Plant Genetic Conservation Project under The Royal Initiative of Her Royal Highness Princess Maha Chakri Sirindhorn area, Chulabhorn Dam, Konsan district within Chaiyaphum province in Thailand. The strain was identified via analysis of nucleotide sequences from the D1/D2 domain of 26S ribosomal DNA and based on its morphological, physiological and biochemical characteristics. The sequence obtained from yeast isolate KKU-FW10 was 97 percent identical to that of Candida chanthaburiensis (GenBank accession number AB500861.1), with 506/517 (nucleotides identity/total nucleotides) matching nucleotides, nine substitutions and two gaps being detected. This species belonged to the Candida clade. Regarding morphological characteristics, isolate KKU-FW10 presents cream-colored butyrous colonies, vegetative reproduction through budding and, round cells without filaments or ascospores. The major ubiquinone detected was Q-9. The above results suggest that isolate KKU-FW10 is a new member of the genus Candida, and the name Candida konsanensis is proposed for this yeast. The type strain of the new species is KKU-FW10T (= BCC 52588T, = NBRC 109082T, = CBS 12666T). In addition, this KKU-FW10 could potentially produce 58.24 Units/ml of carboxymethyl cellulase when it was cultured in YP broth containing 1.0 % carboxymethyl cellulose for 24 h.

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