Human single-chain variable fragment antibody expressed in E. coli with optimal in vitro cross-neutralizing and no enhancing activity

Biologicals - Tập 56 - Trang 54-62 - 2018
Surachet Benjathummarak1, Chonlatip Pipattanaboon2, Khwanchit Boonha1, Waranya Wongwit3, Pongrama Ramasoota1,3, Pannamthip Pitaksajjakul1,3
1Center of Excellence for Antibody Research (CEAR), Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
2Department of Microbiology, Faculty of Medicine, Khon Kaen University, Thailand
3Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand

Tài liệu tham khảo

Qi, 2008, Biological characteristics of dengue virus and potential targets for drug design, Acta Biochim Biophys Sin, 40, 91, 10.1111/j.1745-7270.2008.00382.x Rice, 1985, Nucleotide sequence of yellow fever virus: implications for flavivirus gene expression and evolution, Science, 229, 726, 10.1126/science.4023707 Guzman, 2010, Dengue: a continuing global threat, Nat Rev Microbiol, 8, S7, 10.1038/nrmicro2460 Kliks, 1989, Antibody-dependent enhancement of dengue virus growth in human monocytes as a risk factor for dengue hemorrhagic fever, Am J Trop Med Hyg, 40, 444, 10.4269/ajtmh.1989.40.444 Whitehead, 2007, Prospects for a dengue virus vaccine, Nat Rev Microbiol, 5, 518, 10.1038/nrmicro1690 Murrell, 2011, Review of dengue virus and the development of a vaccine, Biotechnol Adv, 29, 239, 10.1016/j.biotechadv.2010.11.008 2016, 349 Schieffelin, 2010, Neutralizing and non-neutralizing monoclonal antibodies against dengue virus E protein derived from a naturally infected patient, Virol J, 7, 28, 10.1186/1743-422X-7-28 Dejnirattisai, 2010, Cross-reacting antibodies enhance dengue virus infection in humans, Science, 328, 745, 10.1126/science.1185181 Bernardo, 2009, Antibodies induced by dengue virus type 1 and 2 envelope domain III recombinant proteins in monkeys neutralize strains with different genotypes, Clin Vaccine Immunol, 16, 1829, 10.1128/CVI.00191-09 de Alwis, 2011, In-depth analysis of the antibody response of individuals exposed to primary dengue virus infection, PLoS Negl Trop Dis, 5, e1188, 10.1371/journal.pntd.0001188 de Alwis, 2012, Identification of human neutralizing antibodies that bind to complex epitopes on dengue virions, Proc Natl Acad Sci U S A, 109, 7439, 10.1073/pnas.1200566109 Teoh, 2012, The structural basis for serotype-specific neutralization of dengue virus by a human antibody, Sci Transl Med, 4, 10.1126/scitranslmed.3003888 Zou, 2012, Resistance analysis of an antibody that selectively inhibits dengue virus serotype-1, Antivir Res, 95, 216, 10.1016/j.antiviral.2012.06.010 Costin, 2013, Mechanistic study of broadly neutralizing human monoclonal antibodies against dengue virus that target the fusion loop, J Virol, 87, 52, 10.1128/JVI.02273-12 Setthapramote, 2012, Human monoclonal antibodies to neutralize all dengue virus serotypes using lymphocytes from patients at acute phase of the secondary infection, Biochem Biophys Res Commun, 423, 867, 10.1016/j.bbrc.2012.06.057 Sasaki, 2013, Dengue virus neutralization and antibody-dependent enhancement activities of human monoclonal antibodies derived from dengue patients at acute phase of secondary infection, Antivir Res, 98, 423, 10.1016/j.antiviral.2013.03.018 Presta, 2003, Antibody engineering for therapeutics, Curr Opin Struct Biol, 13, 519, 10.1016/S0959-440X(03)00103-9 Farajnia, 2014, Development trends for generation of single-chain antibody fragments, Immunopharmacol Immunotoxicol, 36, 297, 10.3109/08923973.2014.945126 Kurosu, 2010, Highly efficient rescue of dengue virus using a co-culture system with mosquito/mammalian cells, Biochem Biophys Res Commun, 394, 398, 10.1016/j.bbrc.2010.02.181 Pitaksajjakul, 2014, Antibody germline characterization of cross-neutralizing human IgGs against 4 serotypes of dengue virus, Biochem Biophys Res Commun, 446, 475, 10.1016/j.bbrc.2014.02.131 Palmer, 2004, Preparation and extraction of insoluble (inclusion-body) proteins from Escherichai coli, Curr Protoc Protein Sci, Chapter 6, 10.1002/0471140864.ps0603s38 Bu, 2013, Expression and purification of a novel therapeutic single-chain variable fragment antibody against BNP from inclusion bodies of Escherichia coli, Protein Expr Purif, 92, 203, 10.1016/j.pep.2013.10.002 Konishi, 2010, A simple assay system for infection-enhancing and -neutralizing antibodies to dengue type 2 virus layers of semi-adherent K562 cells, J Virol Methods, 163, 360, 10.1016/j.jviromet.2009.10.026 Chan, 2013, Therapeutic antibodies as a treatment option for dengue, Expert Rev Anti Infect Ther, 11, 1, 10.1586/14787210.2013.839941 Poungpair, 2014, Generation of human single-chain variable fragment antibodies specific to dengue virus non-structural protein 1 that interfere with the virus infectious cycle, mAbs, 6, 474, 10.4161/mabs.27874 Saokaew, 2014, Human monoclonal single-chain antibodies specific to dengue virus envelope protein, Lett Appl Microbiol, 58, 270, 10.1111/lam.12186 Boss, 1984, Assembly of functional antibodies from immunoglobulin heavy and light chains synthesised in E. coli, Nucleic Acids Res, 12, 3791, 10.1093/nar/12.9.3791 Nelson, 2010, Antibody fragments: hope and hype, mAbs, 2, 77, 10.4161/mabs.2.1.10786 Ahmad, 2012, scFv antibody: principles and clinical application, Clin Dev Immunol, 2012, 980250, 10.1155/2012/980250 Francis, 2010, Strategies to optimize protein expression in E. coli, Curr Protoc Protein Sci, 5, 10.1002/0471140864.ps0524s61 Bhatia, 2010, Single-chain fragment variable antibody against the capsid protein of bovine immunodeficiency virus and its use in ELISA, J Virol Methods, 167, 68, 10.1016/j.jviromet.2010.03.012 Lobstein, 2012, SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded protein in its cytoplasm, Microb Cell Fact, 11, 1, 10.1186/1475-2859-11-56 Cheng, 2014, Functional production of a soluble and secreted single-chain antibody by a bacterial secretion system, PLoS One, 9, e97367, 10.1371/journal.pone.0097367 Kipriyanov, 1996, Affinity enhancement of a recombinant antibody: formation of complexes with multiple valency by a single-chain Fv fragment-core streptavidin fusion, Protein Eng, 9, 203, 10.1093/protein/9.2.203 Smallshaw, 1999, Synthesis, cloning and expression of the single-chain Fv gene of the HPr-specific monoclonal antibody, Jel42: determination of binding constants with wild-type and mutant HPrs, Protein Eng, 12, 623, 10.1093/protein/12.7.623 Robinson, 2014, Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria, Nat Commun, 6, 1 Clark, 1998, Refolding of recombinant proteins, Curr Opin Biotechnol, 9, 157, 10.1016/S0958-1669(98)80109-2 Kudou, 2011, Refolding single-chain antibody (scFv) using lauroyl-L-glutamate as a solubilization detergent and arginine as a refolding additive, Protein Expr Purif, 77, 68, 10.1016/j.pep.2010.12.007 Yamaguchi, 2014, Refolding techniques for recovering biologically active recombinant proteins from inclusion bodies, Biomolecules, 4, 235, 10.3390/biom4010235 Eiberle, 2010, Technical refolding of proteins: do we have freedom to operate?, Biotechnol J, 5, 547, 10.1002/biot.201000001 Byers, 2015, Influence of FcγRIIa-expressing cells on the assessment of neutralizing and enhancing serum antibodies elicited by a live-attenuated tetravalent dengue vaccine, OFID, 2, ofv172, 10.1093/ofid/ofv172 Tanu, 2013, Dengue virus neutralization in cells expressing Fc gamma receptors, PLoS One, 8, 1 Klein, 2009, Examination of the contributions of size and avidity to the neutralization mechanisms of the anti-HIV antibodies b12 and 4E10, Proc Natl Acad Sci Unit States Am, 106, 7385, 10.1073/pnas.0811427106 Wahala, 2011, The human antibody response to dengue virus infection, Viruses, 3, 2374, 10.3390/v3122374 Tsai, 2013, High-avidity and potently neutralizing cross-reactive human monoclonal antibodies derived from secondary infection, J Virol, 87, 12562, 10.1128/JVI.00871-13 Lin, 2012, Analysis of epitopes on dengue virus envelope protein recognized by monoclonal antibodies and polyclonal human sera by a high throughput assay, PLoS Negl Trop Dis, 6, 1, 10.1371/journal.pntd.0001447 Huang, 2010, The dengue virus type 2 envelope protein fusion is essential for membrane fusion, Virology, 396, 305, 10.1016/j.virol.2009.10.027 Williams, 2013, Therapeutic efficacy of antibodies lacking Fcγ receptor binding against lethal dengue virus infection is due to neutralizing potency and blocking of enhancing antibodies, PLoS Pathog, 9, 10.1371/journal.ppat.1003157