Effect of thrombopoietin on peroxidase activity of cryopreserved peripheral blood stem cells
Tóm tắt
Transplantation of mobilized peripheral blood stem cells is increasingly used to facilitate hematological recovery following high-dose chemotherapy. In general, peripheral blood stem cells can be maintained by cryopreservation, e.g., at −80°C, until the day of transplantation. We investigated the effect of thrombopoietin (c-Mpl ligand) on cryopreserved peripheral blood stem cells obtained from 10 patients with malignant disease by assessing peroxidase activity by ultrastructual technique. Leukocyte surface markers wre evaluated by flow cytometry. After peripheral blood stem cells had been purified by use of CD34 monoclonal antibody, cells whose size resembled that of monocytes showed low levels of positivity for CD7, CD41a, and CD42b, but high levels of positivity for CD13, CD14, and HLA-DR. The lymphocyte-size cells showed high levels of positivity for CD7 only. Recombinant human thrombopoietin, administered alone and in combination with various cytokines, promoted the expression of CD41a by CD34-positive progenitor cells. No increase in the expression of CD7, CD13, CD14, and HLA-DR was detected following exposure to cytokines. However, the expression of CD41a and CD42b was enhanced by thrombopoietin. Ultrastructural study of peroxidase activity detected CD41a-positive cells. as well as platelet peroxidase-positive cells, following stimulation with thrombopoietin. Stimulation by other cytokines failed to yield platelet peroxidase-positive cells. Results thus suggest that thrombopoietin may be useful for increasing the recovery of platelets following transplantation of cryopreserved peripheral blood stem cells.
Tài liệu tham khảo
Robinson BE, Mcgrath HE, Quesenberry PJ (1987) Recombinant murine granulocyte macrophage colony-stimulating factor has megakaryocyte colony-stimulating activity and augments megakaryocyte colony stimulation by interleukin-3. J Clin Invest 79:1648–1652
Ishibashi T, Shikama Y, Kimura H, Kawaguchi M, Uchida T, Yamamoto T, Okano A, Akiyama Y, Hirano T, Kishimoto T, Maruyama Y (1993). Thrombopoietic effects of interleukin-6 in long-term administration in mice. Exp Hematol 21:640–646
Burstein SA, Mei R-L, Henthorn J, Friese P, Turner K (1992) Leukemia inhibitory factor and interleukin-11 promote maturation of murine and human megakaryocytes in vitro. J Cell Physiol 153:305–312
Souyri M, Vignon I, Penciolelli J-F, Heard J-M, Tambourin P, Wendling F (1990) A putative truncated cytokine receptorgene transduced by the myeloproliferative leukemia virus immortalizes hematopoietic progenitors. Cell 63:1137–1147
de Sauvage FJ, Hass PE, Spencer SD, Malloy BE, Gurney AL, Spencer SA, Darbonne WC, Henzel WJ, Wong SC, Kuang W-J, Oles KJ, Hultgren B, Solberg LA, Goeddel DV, Eaton DL (1994) Stimulation of megakaryocytopoiesis and thrombopoiesis by the cMpl ligand. Nature 369:533–538
Kaushansky K, Lok S, Holly RD, Broudy VC, Lin N, Bailey MC, Forstrom JW, Buddle MM, Oort PJ, Hagen FS, Roth GJ, Papayannopoulou T, Foster DC (1994) Fromotion of megakaryocyte progenitor expansion and differentiation by the c-Mpl ligand thrombopoietin. Nature 369:568–571
Kaushansky K (1995) Thrombopoietin: the primary regulator of platelet production. Blood 86:419–431
Juttner CA, To LB, Haylock DN, Dyson PG, Thorp D, Dart GW, Ho JQK, Horvath N, Bardy P (1989) Autologous blood stem cell transplantation. Transplant Proc 21:2929–2931
Haas R, Ho AD, Bredthauer U, Gayeux S, Egerer G, Knauf W, Hunstein W (1990) Successful autologous transplantation of blood stem cells mobilized with recombinant human granulocytemacrophage colony-stimulating factor. Exp Hematol 18:94–98
Elias AD, Ayash L, Anderson KC, Hunt M, Wheeler C, Schwartz G, Tepler I, Mazanet R, Lynch C, Pap S, Pelaez J, Reich E, Critchlow J, Demetri G, Bibbo J, Schnipper L, Griffin JD, Frei E III, Antma KH (1992) Mobilization of peripheral blood progenitor cells by chemotherapy and granulocyte-macrophage colonystimulating factor for hematologic support after high-dose intensification in breast cancer. Blood 79:3036–3044
Brugger W, Bross KJ, Frisch J, Dern P, Weber B, Mertelsmann R, Kanz L (1992) Mobilization of peripheral blood progenitor cells by sequential administration of IL-3 and GM-CSF following polychemotherapy with etoposide, ifosfamide, and cisplatin. Blood 79:1193–1200
Sheridan WP, Begley CG, Juttner C, Szer J, To LB, Maher D, Mcgrath KM, Morstyn G, Fox RM (1992) Effect of peripheral-blood grogenitor cells mobilised by figrastim (G-CSF) on platelet recovery after high-dose chemotherapy. Lancet 339:640–644
Bender JG, Unverzagl K, Walker D (1994) Guidelines for determination of CD34+ cells by flow cytometry: application to the harvesting and transplantation of peripheral blbod stem cells. In: Wunder E, Sovalat H, Henor R, Serke S (eds) Hematopoietic stem cells, the Mulhouse manual. Alpha Med, Dayton, pp 31–43
Civin CL (1989) Summary of CD34 clustey workshop section. In: Knapp W (ed) Leukocyte typing, vol IV. Academic, London, p 818
Mercolino TJ, Connelly MC, Meyer EJ, Knight MD, Parker JN, Stelzer GT, Dechirico G (1995) Immunologic differentiation of absolute lymphocyte count with an integrated flow cytometric system: a new concept for absolute T cell subset determinations. Cytometry 22:48–59
Miltenyi S, Guth S, Radbrush A, Pfluger E, Thiel A (1994) Isolation of CD34+ hematopoietic progenitor cells by high-gradient magnetic cell sorting (MACS). In: Wunder E, Sovalat H, Henon P, Serke S (eds) Hematopoietic stem cells, the Mulhouse manual. Alpha Med. Dayton, pp 201–213
Sohma Y, Akahori H, Seki N, Hori T, Ogami K, Kato T, Shimada Y, Kawamura K, Miyazaki H (1994) Molecular cloning and chromoscmal localization of the human thrombopoietin gene. FEBS Lett 353:57–61
Kato T, Ogami K, Shimada Y, Iwamatsu A, Sohma Y, Akahori H, Horie K, Kokubo A, Kudo Y, Maeda E, Kobayashi K, Ohashi H, Ozawa T, Inoue H, Kawamura K, Miyazaki H (1995) Purification and characterization of thrombopoietin. J Biochem (Tokyo) 118:229–236
Graham RC, Karnovsky MJ (1996) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302
Anderson WA, Trantalis J, Kang YH (1975) Ultrastructural localization of endogenous mammary gland peroxidase during lactogenesis in the rat: results after tannic acid-formaldehydeglutaraldehyde fixation. J Histochem Cytochem 23:295–302
Breton-Gorius J, Vanhaeke D, Pryzwansky KB, Guichard J, Tabilic A, Vainchenker W, Carmel R (1984) Simultaneous detection of membrane markers with monoclonal antibodies and peroxidatic activities in leukemia: ultrastructural analysis using a new method of fixation preserving the platelet peroxidase. Br J Haemato 58:447–458
Pryzwansky KB, Schliwa M, Porter KR (1983) Comparison of the three-dimensional organization of unextracted and Tritonextracted human neutrophilic polymorphonuclear leukocytes. Eur J Cell Biol 30:112–125
Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43
Block KL, Poncz M (1995) Platelet glycoprotein IIb gene expression as a model of megakaryocyte-specific expression. Stem Cells (Basel) 13:135–145
Gordon MS, Hotmann R (1992) Growth factors affecting human thrombopoiesis: potential agents for the treatment of thrombocytopenias. Blood 80:302–307
Hoffmann R (1989) Regulation of megakaryocytopoiesis. Blood 74:1196–1212
Segal GM, Stuever T, Adamson JW (1988) Analysis of murine megakaryocyte colony size and ploidy: effects of interleukin-3. J Cell Physiol 137:537–544
Williams N, De Giorgio T, Banu N, Withy R, Hirano T, Kishimoto T (1990) Recombinant interleukin 6 stimulates immature murine megakaryocytes. Exp Hematol 18:69–72
Musashi M, Yang Y-C, Paul SR, Clark SC, Sudo T, Ogawa M (1991) Direct and synergistic effects of interleukin 11 on murine hemopotesis in culture. Proc Natl Acad Sci USA 88:765–769
Bridell RA, Bruno E, Cooper RJ, Brandt JE, Hoffmann R (1991) Effect of c-kit ligand on in vitro human megakaryocytopoiesis. Blood 78:2854–2859
Zucker-Franklin D, Kaushansky K (1996) Effect of thrombopoietin on the development of megakaryocytes and platelets: an ultrastructural analysis. Blood 88:1632–1638
Sato T, Fuse A, Eguchi M, Hayashi Y, Ryo R, Adachi M, Kishimoto Y, Terada M, Mizoguchi H, Shima Y, Komori I, Sunami S, Okimoto Y, Nakajima H (1989) Establishment of a human leukemic cell line (CMK) with megakaryocytic characteristics from a Down's syndrome patient with acute megakaryocytoblastic leukemia. Br J Haematol 72:184–190
Nagano T, Ohga S, Kishimoto Y, Kimura T, Yasumaga K, Adachi M, Ryo R, Sato T (1992) Ultrastructural analysis of platelet-like particles from a human megakaryocytic leukemia cell line (CMK 11-5). Int J Hematol 56:67–78