Xiaoxia Hao1, Chunyan Li1, Yejun Zhang1, Haozhi Wang1, Guangcun Chen1, Mao Wang1, Qiangbin Wang1
1CAS Key Laboratory of Nano‐Bio Interface Division of Nanobiomedicine and i‐Lab CAS Center for Excellence in Brain Science Suzhou Institute of Nano‐Tech and Nano‐Bionics Chinese Academy of Sciences Suzhou 215123 China
Tóm tắt
AbstractCombined chemotherapy and immunotherapy have demonstrated great potential in cancer treatment. However, it is difficult to provide clear information of the pharmacokinetics and pharmacodynamics of chemodrugs and transplanted immune cells in vivo by traditional approaches, resulting in inadequate therapy. Here, a multiplexed intravital imaging strategy by using fluorescence in the second near‐infrared window (NIR‐II) is first developed to visualize the two events of chemotherapy and immunotherapy in vivo, so that a combinational administration is programed to improve the therapeutical effects against a mouse model of human breast cancer. In detail, Ag2Se quantum dots (QDs) (λEm = 1350 nm) loaded with stromal‐cell‐derived factor‐1α (SDF‐1α) and chemodrug doxorubicin (DOX) are first administrated to deliver the SDF‐1α and DOX to the tumor site. After their arrival, monitored by Ag2Se QD fluorescence, natural killer (NK)‐92 cells labeled with Ag2S QDs (λEm = 1050 nm) are intravenously injected so that the cells are recruited to the tumor by the chemotaxis of SDF‐1α, which is visualized by Ag2S QD fluorescence. Such an imaging approach allows simultaneous evaluation of the behaviors of individual injections in vivo, and facilitates optimized administration regimens, resulting in enhanced tumor inhibition.
