Wheat plant DNA methylation pattern changes at chronic seed γ irradiation
Tóm tắt
Alterations of DNA methylation patterns of two wheat sorts Al’batros odesskii and Donetskaya-48 whose seeds were irradiated with a low dose rate (3 × 10−7 Gy/s) for 4 months have been studied. Six restriction endonucleases were used in the experiments. Primary distinction in DNA methylation patterns of the studied sorts has been demonstrated. Chronic irradiation resulted in an increase of the methylation level on the recognion site for GluI and Sou3AI and in a decrease of this index in recognion sites for endonucleases GlaI and HpaII. The alterations of the methylation level in recognition sites for restrictases MboI and MspI were not found. The considerable increase of chromosome aberration level at the same dose of chronic irradiation has been shown. The role of DNA methylation pattern changes in development of irradiation damage and organism protective reactions is discussed.
Tài liệu tham khảo
Shafirkin, A.V. and Gorlov, V.G., Model Description of the Kinetics of the Effective Body Lesion in Prolonged and Chronic Radiation Exposures, Radiobiologiya, 1978, vol. 18, no. 2, pp. 253–258.
Kravets, A.P., Gatilova, G.D., and Grodzinskii, D.M., The Dynamics of Cytogenetic Anomalies Output in Seedling Merysteme at the Chronic Irradiation of Seeds, Radiats. Biol. Radioekol., 2008, vol. 48, no. 3, pp. 208–219.
Vasin, A.L. and Shafirkin, A.V., The Quantitative Criteria of the Transition from Norm to Pathology at Chronic Exposure of Physical Factors, Radiats. Biol. Radioekol., 2006, vol. 46, no. 4, pp. 498–507.
Tishchenko, E.N. and Dubrovnaya, O.V., Epigeneticheskaya regulyatsiya. Metilirovanie DNK genov i transgenov rastenii (Epigenetic Regulation. Methylation of DNA of Plant Genes and Transgenes), Kiev: Logos, 2004.
Pogribny, I., Koturbash, I., Tryndyak, V., Hudson, D., Stevenson, S.M.L., Sedelnikova, O., Bonner, W., and Kovalchuk, O., Fractionated Low-Dose Radiation Exposure Leads to Accumulation of DNA Damage and Profound Alterations in DNA and Histone Methylation in the Murine Thymus, Mol. Cancer Res., 2005, vol. 3, pp. 553–561.
Bernal, A., Dolinoy, D.C., Huang, D., and Jirtle, R.L., Low Dose Radiation Alters the Fetal Epigenome (http://www.orau.gov/lowdose2009/abstracts/Jirtle_Randay.pdf).
Kim, S.Y., Yun, H.J., Kwon, Y.Y., Kim, E.J., and Kang, C.M., Possible Biomarkers for Low Dose Radiation Exposure and/or for Old Exposure (http://www.dartmouth.edu/~eprctr/biodose2008/pdf/B10.pdf).
Dyachenko, O.V., Zakharchenko, N.S., Shevchuk, T.V., Bohnert, H.J., Cushman, J.C., and Buryanov, Ya.I., Effect of Hypermethylation of CCWGG Sequences in DNA, Biochemistry, 2006, vol. 71, no. 4, pp. 461–465.
Kovalchuk, O., Burke, P., Arkhipov, A., Kuchma, N., James, S.J., Kovalchuk, I., and Pogribny, I., Genome Hypermethylation in Pinus sylvestris of Chernobyl—A Mechanism for Radiation Adaptation?, Mutat. Res., 2003, vol. 529, nos. 1/2, pp. 13–20.
Vagin, V.V., Sigova, A., Li, Ch., Herve, Seitz H., Gvozdev, V., and Zamore, P.D., A Distinct Small RNA Pathway Silences Selfish Genetic Elements in the Germline, Science, 2006, no. 5785, pp. 320–324.
Aravin, A.A., Sachidanandam, R., Girard, A., Fejes-Toth, K., and Hannon, G.J., Developmentally Regulated piRNA Clusters Implicate MILI in Transposon Control, Science, 2007, vol. 316, pp. 744–747.
Brooks, A.L., Biomarkers of Exposure, Sensitivity and Disease, Int. J. Radiat. Biol., 1999, vol. 75, no. 12, pp. 1481–1503.