Agrobacterium-mediated transformation of Citrus stem segments and regeneration of transgenic plants
Tóm tắt
A method for Agrobacterium-mediated transformation of Citrus and organogenic regeneration of transgenic plants is reported. Internodal stem segments were co-cultured with Agrobacterium harboring binary vectors that contained the genes for the scorable marker ß-glucuronidase (GUS) and the selectable marker NPT-II. A low but significant percentage (≤ 5%) of the shoots regenerated in the presence of 100 μg/ml kanamycin were GUS+. Polymerase chain reaction (PCR) analysis confirmed that GUS+ shoots contained T-DNA. Two plants established in soil were shown to be transgenic by Southern analysis.
Tài liệu tham khảo
An G (1987) Methods Enzymol 153:292–305
Watson BD, Stachel S, Gordon MP, Nester EW (1985) EMBO J 4:277–284
Church GM, Gilbert W (1984) Proc Natl Acad Sci 81:1991–1995
Dellaporta SL, Wood J, Hicks JB (1983) Plant Mol Biol Reporter 1:19–21
Durham RE (1990) PhD Dissertation, University of Florida
Gmitter FG, Moore GA (1986) Cell Tissue and Organ Culture 6:139–147
Hidaka T, Omura M, Ugaki M, Tomiyama M, Kato A, Ohshima M, Motoyoshi F (1990) Japan J Breed 40:199–207
Hood EE, Helmer GL, Fraley RT, Chilton M-D (1986) J Bacteriol 168:1291–1301
Horsch RB, Fry JE, Hoffmann NL, Eichholtz D, Rogers SG, Fraley RT (1985) Science 227:1229–1231
Jayaswal RK, Veluthambi K, Gelvin SB, Slightom, JL (1987) J Bacteriol 169:5035–5045
Jefferson RA (1987) Plant Mol Biol Reporter 5:387–405
Jordan MC, McHughen A (1988) Plant Cell Rep 7:285–287
Knbayashi S, Uchimiya H (1989) Japan J Genet 64:91–97
Komari T, Halperin W, Nester EW (1986) J Bacteriol 166:88–94
Lassner MW, Peterson P, Yoder JI (1989) Plant Mol Biol Reporter 7:116–128
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York.
Murashige T, Tucker DPH (1969) Proc First Inti Citrus Symp 3:1155–1161
Rogers, SG, Klee HJ, Horsch RB, Fraley RT (1987) Meth Enzymol 153:253–277
Rogers SO, Bendich AJ (1985) Plant Mol Biol 5:69–76
Vardi A, Bleichman S, Aviv D (1990) Plant Sci 69:199–206