Leukocyte infiltration in synovial tissue from the shoulder of patients with polymyalgia rheumatica. Quantitative analysis and influence of corticosteroid treatment

Wiley - Tập 39 Số 7 - Trang 1199-1207 - 1996
Riccardo Melicòni1, Lia Pulsatelli2, Mariagrazia Uguccioni3, Carlo Salvarani4, Pierluigi Macchioni4, Cinzia Melchiorri5, Maria Cristina Focherini2, Luigi Frizziero2, Andrea Facchini6
1University of Bologna, Italy
2Ospedale Maggiore, Bologna, Italy
3Theodor Kocher Institut, Bern, Switzerland
4Arcispedale S. Maria Nuova, Reggio Emillia, Italy
5Istituto Codivilla Putti–IOR, Bologna, Italy
6University of Bologna, and Istituto Codivilla Putti–IOR, Bologna, Italy

Tóm tắt

AbstractObjective: To investigate the immunologic features of synovitis in patients with polymyalgia rheumatica (PMR) and to assess the modifications induced by corticosteroid.Methods. Arthroscopic biopsies of shoulder synovium were obtained from 12 patients with untreated PMR and from 7 patients with PMR that had been treated. Immunohistochemistry was performed on frozen sections utilizing a panel of monoclonal antibodies and computerized image analysis.Results. Synovitis was present in 10 of 12 (83%) untreated patients and in only 2 of 7 (29%) treated patients. The synovitis was characterized by vascular proliferation and leukocyte infiltration. Infiltrating cells consisted predominantly of macrophages and T Lymphocytes. Almost all T lymphocytes were CD45RO positive. A few neutrophils, but no B cells, natural killer cells, or γ/δ T cells were found. Intense expression of HLA class II antigens (DR moreso than DP moreso than DQ) was found in the lining layer cells as well as in macrophages and lymphocytes. DR, but not DP or DQ, was expressed by the endothelium of a few vessels. Class II antigen expression correlated with the number of macrophages and lymphocytes. Macrophage infiltration of arteriole walls was observed in 1 untreated patient without giant cell arteritis (GCA). In untreated patients, there was a positive correlation between the percentage of infiltrating T cells and the duration of disease. Steroid therapy was associated with a significant reduction in the number of blood vessels and of HLA class II expression. One treated patient who no longer had symptoms of PMR still had active synovitis: a relapse occurred 4 months after the biopsy.Conclusion. Our findings support the hypothesis that synovitis is a major cause of the musculoskeletal symptoms of PMR. There are immunologic similarities with the vascular inflammation observed in GCA. Corticosteroids act on both the vascular and cellular components of synovitis.

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Tài liệu tham khảo

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Wiley

Tập 39 Số 7

1199-1207

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