Diversity of culturable yeasts in phylloplane of sugarcane in Thailand and their capability to produce indole-3-acetic acid
Tóm tắt
Yeasts were isolated by the enrichment technique from the phylloplane of 94 samples of sugarcane leaf collected from seven provinces in Thailand. All sugarcane leaf samples contained yeasts and 158 yeast strains were obtained. On the basis of the D1/D2 domain of the large subunit rRNA gene sequence analysis, 144 strains were identified to 24 known species in 14 genera belonging to the Ascomycota viz. Candida akabanensis, Candida dendronema, Candida mesorugosa, Candida michaelii, Candida nivariensis, Candida rugosa, Candida orthopsilosis, Candida quercitrusa, Candida tropicalis, Candida xylopsoci, Cyberlindnera fabianii, Cyberlindnera rhodanensis, Debaryomyces nepalensis, Hannaella aff. coprosmaensis, Hanseniaspora guilliermondii, Kluyveromyces marxianus, Lachancea thermotolerans, Lodderomyces elongisporus, Metschnikowia koreensis, Meyerozyma caribbica, Millerozyma koratensis, Pichia kudriavzevii, Torulaspora delbrueckii and Wickerhamomyces edaphicus, and 12 species in six genera of the Basidiomycota viz
. Cryptococcus flavescens, Cryptococcus laurentii, Cryptococcus rajasthanensis, Kwoniella heveanensis, Rhodosporidium fluviale, Rhodosporidium paludigenum, Rhodotorula mucilaginosa, Rhodotorula sesimbrana, Rhodotorula taiwanensis, Sporidiobolus ruineniae, Sporobolomyces carnicolor and Sporobolomyces nylandii. Seven strains were identical or similar to four undescribed species. Another seven strains represented four novels species in the genus Metschnikowia, Nakazawaea, Wickerhamomyces and Yamadazyma. The results revealed 69 % of the isolated strains were ascomycete yeasts and 31 % were basidiomycete yeast. The most prevalent species was M. caribbica with a 23 % frequency of occurrence followed by Rh. taiwanensis (11 %) and C. tropicalis (10 %). All strains were assessed for indole-3-acetic acid (IAA) producing capability showing that 69 strains had the capability of producing IAA when cultivated in yeast extract peptone dextrose broth supplemented with 1 g/L l-tryptophan. The highest IAA concentration of 565.1 mg/L was produced by R. fluviale DMKU-RK253.
Tài liệu tham khảo
Altschul SF, Madden TL, Schäffer JZ, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402
Andrews JH, Harris RE (2000) The ecology and biogeography of microorganisms on plant surfaces. Ann Rev Phytopathol 38:145–180
Bilkay IS, Karakoc S, Aksoz N (2010) Indole-3-acetic acid and gibberellic acid production in Aspergillusniger Turk. J Biol 34:313–318
Chaihara M, Lumyong S (2011) Screening and optimization of indole-3-acetic acid production and phosphate solubilization from Rhizobacteria aimed at improving plant growth. Curr Microbiol 623:173–181
Cleland RE (1990) Auxin and cell elongation. In: Davies PJ (ed) Plant hormones and their role in plant growth and development. Kluwer, Dordreecht, pp 132–148
de Azeredo LAI, Gomes EAT, Mendonca-Hagler LC, Hagler AN (1998) Yeast communities associated with sugarcane in Campos. Rio de Janeiro Brazil. Internatl Microbiol 1:205–208
Delker C, Raschke A, Quint M (2008) Auxin dynamics: the dazzling complexity of a small molecule’s message. Planta 227:929–941
El-Tarabily KA (2004) Suppression of Rhizoctonia solani diseases of sugar beet by antagonistic and plant growth-promoting yeasts. J Appl Microbiol 96:69–75
Fiala V, Glad C, Martin M, Jolivet E, Derridj S (1900) Occurrence of soluble carbohydrates on the phylloplane of maize (Zea mays L.): Variations in relation to leaf heterogeneity and position on the plant. New Phytol 115:609–615
Fonseca A, Inacio J (2006) Phylloplane yeasts. In: Rosa C, Peter G (eds) Biodiversity and ecophysiology of yeasts. Springer, Berlin, pp 263–301
Glickmann E, Dessaux Y (1994) A critical examination of the specificity of the Salkowski reagent for indolic compounds produced by phytopathogenic bacteria. Appl Environ Microbiol 61:793–796
Glushakova AM, Chernov IY (2010) Seasonal dynamics of the structure of epiphytic yeast communities. Microbiology 79:830–839
Inácio J, Portugal L, Spencer-Martins I, Fonseca A (2005) Phylloplane yeasts from Portugal: seven novel anamorphic species in the Tremellales lineage of the Hymenomycetes (Basidiomycota) producing orange-coloured colonies. FEMS Yeast Res 5:1167–1183
Kaewwichian R, Limtong S (2013) Nakazawaea siamensis f.a., sp. nov., a novel yeast species isolated from phylloplane in Thailand. Int J Syst Evol doi:10.1099/ijs.0.057521-0
Kaewwichian R, Yongmanitchai W, Kawasaki H, Limtong S (2012) Metschnikowia saccharicola sp. nov. and Metschnikowia lopburiensis sp. nov., two novel yeast species isolated from phylloplane in Thailand. Antonie Van Leeuwenhoek 102:743–751
Kaewwichian R, Kawasaki H, Limtong S (2013a) Wickerhamomyces siamensis sp. nov., a novel yeast species isolated from phylloplane in Thailand. Int J Syst Evol Microbiol 63:1568–1573
Kaewwichian R, Yongmanitchai W, Kawasaki H, Wang P-H, Yang S-H, Limtong S (2013b) Yamadazyma siamensis sp. nov., Yamadazyma phyllophila sp. nov. and Yamadazyma paraphyllophila sp. nov., three novel yeast species isolated from phylloplane in Thailand and Taiwan. Antonie Van Leeuwenhoek 103:777–788
Khamna S, Yokota A, Peberdy JF, Lumyong S (2010) Indole-3-acetic acid production by Streptomyces sp. isolated from some Thai medicinal plant rhizospheres soils. EurAsia J BioSci 4:23–32
Kim YK, Oh YJ, Park WJ (2006) HPLC-based quantification of indole-3-acetic acid in the primary root tip of maize. J Nano Biotech 3:40–45
Kurtzman CP, Robnett CJ (1998) Identification and phylogeny of ascomycete yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek 73:331–371
Landell MF, Billodre R, Ramos JP, Leoncini O, Vainstein MH, Valente P (2010) Candida aechmeae sp. nov. and Candida vrieseae sp. nov., novel yeast species isolated from the phylloplane of bromeliads in Southern Brazil. Int J Syst Evol Microbiol 60:244–248
Limtong S, Koowadjanakul N (2012) Yeasts from phylloplanes and their capability to produce indole-3-acetic acid. World J Microbiol Biotechnol 28:3323–3335
Limtong S, Yongmanitchai W, Tun MM, Kawasaki H, Seki T (2007) Kazachstania siamensis sp. nov., an ascomycetous yeast species from forest soil in Thailand. Int J Syst Evol Microbiol 57:419–422
Lindow SE, Brandl MT (2003) Microbiology of the phyllosphere. Appl Environ Microbiol 69:1875–1883
Mujahid M, Sasikala C, Ramana C (2011) Production of indole-3-acetic acid and related indole derivatives from l-tryptophan by Rubrivivax benzoatilyticus JA2. Appl Microbiol Biotechnol 89:1001–1008
Nakamura T, Murakami T, Saotome M, Tomita K, Kitsuwa T, Meyers SP (1991) Identification of indole-3-acetic acid in Pichia spartina an ascosporogenous yeast from Spartina alterniflora marshland environments. Mycologia 83:662–664
Nakase T, Takashima M, Itoh M, Fungsin B, Potacharoen W, Atthasampunna P, Komagata K (2001) Ballistoconidium-froming yeasts found in the phyllosphere of Thailand. Microbiol Cult Coll 17:23–33
Nassar AH, El-Tarabily KA, Sivasithamparam K (2005) Promotion of plant growth by an auxin-producing isolate of the yeast Williopsis saturnus endophytic in maize (Zea mays L.) roots. Biol Fertil Soils 42:97–108
Olasupo NA, Bakre S, Teniola OD, James SA (2003) Identification of yeasts isolated from Nigerian sugar cane peels. J Basic Microbiol 43:530–533
Perondi NL, Luz WC, Thomas R (1996) Microbiological control of Gibberella in wheat. Fitopatol Bras 21:243–2496
Phaff HJ, Starmer WT (1987) Yeasts associated with plants, insects and soil. In: Rose AH, Harrison JS (eds) The yeasts, 2nd edn. Academic, London, pp 123–180
Prusty R, Grisafi P, Fink GR (2004) The plant hormone indole acetic acid induces invasive growth in Saccharomyces cerevisiae. Proc Nalt Acad Sci USA 101:4153–4157
Rao RP, Hunter A, Kashpur O, Normanly J (2010) Aberrant synthesis of indole-3-acetic acid in Saccharomyces cerevisiae triggers morphogenic transition, a virulence trait of pathogenic fungi. Genetics 185:211–220
Remans R, Spaepen S, Vanderleyden J (2006) Auxin signaling in plant defense. Science 313:171
Remus-Emsermann MNP, Tecon R, Kowalchuk GA, Leveau JHJ (2012) Variation in local carrying capacity and the individual fate of bacterial colonizers in the phyllosphere. ISME J 6:756–765
Ruanpanun P, Tangchitosomkid N, Hyde KD, Lumyong S (2010) Actinomycetes and fungi isolated from plant-parasitic nematode infested soils: screening of the effective biocontrol potential, indole-3-acetic acid and siderophores production. World J Microbiol Biotechnol 26:1569–1578
Sampaio JP (2011) Sporidiobolus Naland 1949. In: Kurtzman CP, Fell JW, Boekhout T (eds) The yeasts, a taxonomic study, vol 2, 5th edn. Elsevier, Amsterdam, pp 1549–1561
Sasirekha B, Shivakumar S, Sullia SB (2012) Statistical optimization for improved indole-3-acetic acid (iaa) production by Pseudomonas aeruginosa and demonstration of enhanced plant growth promotion. J Soil Sci Plant Nutr 12:863–873
Shin M, Shinguu T, Sano K, Umezawa C (1991) Metabolic fates of l-tryptophan in Saccharomyces uvarum (Saccharomyces carlsbergensis). Chem Pharm Bull (Tokyo) 39:1792–1795
Shrestha P, Szaro TM, Bruns TD, Taylor JW (2011) Systematic search for cultivatable fungi that best deconstruct cell walls of Miscanthus and sugarcane in the field. Appl Environ Microbiol 77:5490–5504
Slavikova E, Vadkertiova R, Vranova D (2009) Yeasts colonizing the leaves of fruit trees. Ann Microbiol 59:419–424
Spaepen S, Vanderleyden J, Remans R (2007) Indole-3-acetic acid in microbial and microorganism-plant signaling. FEMS Microbiol Rev 31:425–448
Takashima M, Nakase T (2000) Four new species of the genus Sporobolomyces isolated from leaves in Thailand. Mycoscience 41:357–369
Tsavkelova EA, Klimova SY, Cherdyntseva TA, Netrusov AI (2006) Microbial producers of plant growth stimulators and their practical use: a review. Appl Biochem Microbiol 42:117–126
Vorholt JA (2012) Microbial life in the phyllosphere. Nat Rev Microbiol 10:828–840
Woodward AW, Bartel B (2005) Auxin: regulation, action, and interaction. Ann Bot (Lond) 95:707–735
Xin G, Glawe D, Doty SL (2009) Characterization of three endophytic, indole-3-acetic acid-producing yeasts occurring in Populus trees. Mycol Res 113:973–980
Xinxian L, Xuemei C, Yagang C, Woon-Chung W, Zebin W, Qitang W (2011) Isolation and characterization endophytic bacteria from hyper accumulator Sedum alfredii Hance and their potential to promote phytoextraction of zinc polluted soil. World J Microbiol Biotechnol 27:1197–1207