Camelina mutants resistant to acetolactate synthase inhibitor herbicides

Molecular Breeding - 2011
Dustin T. Walsh1, Ebrahiem M. Babiker2, Ian C. Burke1, Scot H. Hulbert1,3
1Department of Crop and Soil Sciences, Washington State University, Pullman, USA
2Department of Plant Pathology, Washington State University, Pullman, USA
3Department of Plant Pathology, Washington State University, Pullman, USA.

Tóm tắt

Camelina (Camelina sativa L.) is a low-input oilseed crop of recent interest for sustainable biofuel production. As a relatively new crop in modern agriculture, considerable agronomic and regulatory problems need to be overcome. A common and troublesome problem is sensitivity to residues of acetolactate synthase (ALS) inhibitor herbicides in soils. To develop resistance to those residues, camelina seed were mutagenized by exposure to 0.3% ethyl methane sulfonate and screened at the M2 generation for increased resistance to imazethapyr and sulfosulfuron. Five lines with resistance were identified and characterized. Four mutants, identified in a screen for imazethapyr resistance (IM1, IM6, IM10, and IM18), appeared phenotypically identical and were controlled by the same co-dominant gene. One mutant identified in a screen for sulfosulfuron resistance was phenotypically different but also appears to be controlled by a single co-dominant gene. Further analysis with the IM1 and SM4 mutants confirmed they had increased resistance to imazethapyr, sulfosulfuron, and flucarbazone, with the resistance in the SM4 mutant being the highest. Compared to the wild type, doses of approximately 200 times more imazethapyr, 30 times more sulfosulfuron, and seven times as much flucarbazone were required to reduce plant growth by 50%. Sequence analysis of ALS genes from the SM4 line identified at least eight different genes or alleles. An allele associated with the highest levels of resistance was created by a single base substitution creating an amino acid shift previously found to cause ALS inhibitor resistance in yeast and tobacco.

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Tài liệu tham khảo

Bernasconi P, Woodworth AR, Rosen BA, Subramanian MV, Siehl DL (1995) A naturally occurring point mutation confers broad range tolerance to herbicides that target acertolactate synthase. J Biol Chem 270:17381–17385

Chaleff RS, Mauvals CJ (1984) Acetolactate synthase is the site of action of two sulfonylurea herbicides in higher plants. Science 224:1443–1445

Chaleff RS, Ray TB (1984) Herbicide resistant mutants from tobacco culture. Science 223:1148–1151

Croughan TP (1998) Inventor; board of supervisors of Louisiana State University, assignee. Herbicide resistant rice. U.S. patent 5,773,704

Duggleby RG, Pang SS (2000) Acetohydroxyacid synthase. J Biochem Mol Biol 33:1–36

Duggleby RG, McCourt JA, Guddat LW (2008) Structure and mechanism of inhibition of plant acetohydroxyacid synthase. Plant Physiol Biochem 46:309–324

Eliason R, Schoenau JJ, Szmigielski AM, Laverty WM (2004) Phyotoxicity and persistence of flucabazone-sodium in soil. Weed Sci 52:857–862

Gehringer A, Friedt W, Lühs W, Snowdon RJ (2006) Genetic mapping of agronomic traits in false flax (Camelina sativa subsp. sativa). Genome 49:1555–1563

Gerwick BC, Subramanian MV, Loney-Gallant VI (1990) Mechanism of action of the 1, 2, 4-triazolo[1, 5-a]pryimidines. J Pestic Sci 29:357–364

Hart SE, Saunders JW, Penner D (1992) Chlorsulfuron resistant sugar beet: cross-resistance and physiological basis of resistance. Weed Sci 40:378–383

Haughn GW, Somerville CR (1986) Sulfonylurea-resistant mutants of Arabidopsis thaliana. Mol Gen Genet 204:430–434

Hutcheon C, Ditt RF, Beilstein M, Comai L, Schroeder J, Goldstein E, Shewmaker CK, Nguyen T, De Rocher J, Kiser J (2010) Polyploid genome of Camelina sativa revealed by isolation of fatty acid synthesis genes. BMC Plant Biol 2010(10):233–248

Jung S, Le DT, Yoon S, Yoon MY, Kim YT, Choi J (2004) Amino acid residues conferring herbicide resistance in tobacco acetohydroxyacid synthase. Biochem J 383:53–61

Laplante J, Rajcan I, Tardif FJ (2009) Multiple allelic forms of acetohydroxyacid synthase are responsible for herbicide resistance in Setaria viridis. Theor Appl Genet 119:577–585

Lee Y, Chang AK, Duggleby RG (1999) Effect of mutagenesis synthase at serine 653 of Arabidopsis thaliana acetohydroxyacid synthase on the sensitivity to imidazolinone and sulfonylurea herbicides. FEBS Lett 452:341–345

Neff MM, Turk E, Kalishman M (2002) Web-based primer design for single nucleotide polymorphism analysis. Trends Genet 18:613–615

Newhouse KE, Singh BK, Shaner DL, Stidham MA (1991) Mutations in corn (Zea mays L.) conferring resistance to imidazolinones. Theor Appl Genet 83:65–70

Ott KH, Kwagh GW, Stockton GW, Sidorov V, Kakefude G (1996) Rational molecular design and genetic engineering of herbicide resistant crops by structure modeling and site-directed mutagenesis of acetohyroxyacid synthase. J Mol Biol 263:359–368

Pang SS, Duggleby RD, Guddat LW (2002) Crystal structure of yeast acetohydroxyacid synthase: a target for herbicidal inhibitors. J Mol Biol 317:249–262

Ponziak CJ, Huci PJ (2004) Genetic analysis of imidazolinone resistance in mutation-derived lines of common wheat. Crop Sci 44:23–30

Ponziak CJ, Birk IT, O’Donoughue LS, Menard C, Huci PJ, Singh BK (2004) Physiological and molecular characterization of mutation-derived imidazolinone resistance in spring wheat. Crop Sci 44:1434–1443

Rajasekaran K, Grula JW, Anderson DM (1996) Selection and charachterization of mutant cotton (Gossypium hirsutum L.) cell lines ressistant to sulfonylurea and imidazolinone herbicides. Plant Sci 199:115–124

Sabastian SA, Fader GM, Ulrich DR, Forney DR, Chaleff RS (1989) Semidominant soybean mutation for resistance to sulfonylurea herbicides. Crop Sci 29:1403–1408

Seefeldt SS, Jensen JE, Fuerst EP (1995) Log-logistic analysis of herbicide dose-response relationships. Weed Technol 9:218–227

Senseman SA (2007) Herbicide handbook. Weed Science Society of America, Lawrence, KS

Shaner DL, Anderson PC, Stidham MA (1984) Imidazolinones: potent inhibitors of acetohydroxyacid synthase. Plant Physiol 76:545–546

Singh BK, Stidham MA, Shaner DL (1988) Assay of acetohydroxyacid synthase. Anal Biochem 171:173–179

Stidham MA (1991) Herbicides that inhibit acetohydroxyacid sythase. Weed Sci 39:428–434

Swanson EB, Harrgesel MJ, Arnoldo MJ, Sippell M, Wong RSV (1989) Microspore mutagenesis and selection: canola plants with field resistance to imadozlinone. Theor Appl Genet 78:525–530

Tan S, Evans RR, Dahmer ML, Singh BKShaner DL (2005) Imidazolionone-tolerant crops: history, current status, and future. Pest Manag Sci 61:246–257

Thill DC (1997) Sulfonylurea herbicide resistance in plants. US Patent RE35661

Tranel PJ, Wright TR (2002) Resistance of weeds to ALS-inhibiting herbicides: what have we learned? Weed Sci 50:100–712

Tranel PJ, Wright TR, Heap IM (2011) ALS mutations from herbicide-resistant weeds. http://www.weedscience.org/mutations/mutdisplay.aspx. Last accessed 9 Dec 2011

Vollmanna J, Moritza T, Kargla C, Baumgartnerb S, Wagentristl H (2007) Agronomic evaluation of camelina genotypes selected for seed quality characteristics. Indust Crops Prod 26:270–277

Westerfeld WW (1945) A colorimetric determination of blood acetoin. J Biol Chem 161:495–502

Whaley CM, Wilson HP, Westwood JH (2007) A new mutation in plant ALs confers resistance to five classes of ALS-inhibiting herebicides. Weed Sci 55:83–90

Wright TR, Penner D (1998) Cell celection and inheritance of imidazolinone resistance in sugar beet (Beta vulgaris). Theor Appl Genet 96:612–620

Yu Q, Zhang XQ, Hashem A, Walsh MJ, Powles SB (2003) ALS gener prolene (197) mutations confer ALS herbicide resistance in eight seperate wild radish (Raphanus raphanistrum). Weed Sci 51:831–838

Zubr J (1997) Oil-seed crop: camelina sativa. Indust Crops Prod 6:113–119

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