Attenuation of cadmium toxicity in mycorrhizal celery (Apium graveolens L.)

World Journal of Microbiology and Biotechnology - Tập 23 - Trang 1083-1089 - 2007
Rupam Kapoor1, A. K. Bhatnagar1
1Environmental Biology Laboratory, Department of Botany, University of Delhi, Delhi, India

Tóm tắt

A pot-culture experiment was carried out to investigate the effect of arbuscular mycorrhizal (AM) fungus (Glomus macrocarpum Tul. and Tul.) on plant growth and Cd2+uptake by Apium graveolens L. in soil with different levels of Cd2+. Mycorrhizal (M) and non-mycorrhizal (NM) plants were grown in soil with 0, 5, 10, 40 and 80 Cd2+ mg kg−1soil. The infectivity of the fungus was not affected by the presence of Cd2+ in the soil. M plants showed better growth and less Cd2+ toxicity symptoms. Cd2+ root : shoot ratio was higher in M plants than in NM plants. These differences were more evident at highest Cd2+ level (80 mg kg−1 soil). Chlorophyll a and chlorophyll b concentrations were significantly higher in AM-inoculated celery leaves. The dilution effect due to increased biomass, immobilization of Cd2+ in root and enhanced P-uptake in M plants may be related to attenuation of Cd2+toxicity in celery.

Tài liệu tham khảo

Allen SE (1989) Chemical analysis of ecological materials 2. Blackwell, London Arnon DI (1949) Copper enzymes and isolated chloroplasts polyphenoloxidases in Beta vulgaris. Plant Physiol 24:1–15 Biermann BJ, Linderman RG (1981) Quantifying vesicular arbuscular mycorrhizae: a proposed method towards standardization. New Phytol 89:57–63 Black H (1995) Absorbing possibilities: phytoremediation. Envion Health Prospect 103:1106–1108 Dueck TA, Visser P, Ernst WHO, Sonat H (1986) Vesicular-arbuscular mycorrhiza decrease zinc toxicity to grasses growing in zinc-polluted soil. Soil Biol Biochem 18:331–337 Dufault RJ (1987) Use of slow-release nitrogen and phosphorus fertilizer in celery transplant production. Hort Sci 22:1268–1270 Gaur A, Adholeya A (2004) Propects of arbuscular mycorrhizal fungi in phytoremediation of heavy metal contaminated soils. Curr Sci 86:528–534 Gerdemann JW, Nicolson TH (1963) Spore of mycorrhizal Endogone species from soil by wet sieving and decanting. Trans Br Mycol Soc 46:235–244 Gianinazzi-Pearson V, Gianinazzi S (1983) The physiology of arbuscular-mycorrhizal root. Plant Soil 71:197–209 Gildon A, Tinker PB (1981) A heavy metal-tolerant strain of a mycorrhizal fungus. New Phytol 95:247–261 Gildon A, Tinker PB (1983) Interactions of vesicular-arbuscular mycorrhiza infections and heavy metals in plants II. The effects of infection on uptake of copper. Trans Br Mycol Soc 77:648–649 Giri B, Kapoor R, Mukerji KG (2003) Influence of arbuscular mycorrhizal fungi and salinity on growth, biomass and mineral nutrition of Acacia auriculiformis. Biol Fertil Soils 38:170–175 Heggo A, Angle JS, Charrey RL (1990) Effect of vesicular arbuscular mycorrhizal fungi on heavy-metal uptake by soybeans. Soil Biol Biochem 22:865–869 Heldebrandt U, Kaldrof M, Bothe H (1999) The zinc violet and its colonization by arbuscular myccohizal fungi. J Plant Physiol 154:709–717 Hiscox JD, Israelstem GF (1979) A method for extraction of chlorophyll from leaf tissue without maceration. Can J Bot 57:1332–1334 Jakobsen I, Abbott LK, Robson AD (1992) External hyphae of vesicular-arbuscular mycorrhizal fungi associated with Trifolium subterraneum L. 1. Spread of Hyphae and phosphorus inflow into roots. New Phytol 120:371–380 Joner EJ, Briones R, Leyval C (2000) Metal-binding capacity opf arbuscular mycorrhizal mycelium. Plant Soil 226:227–234 Joner EJ, Leyval C (2001) Time-course of heavy metal uptake in maize and clover as affected by root density and different mycorrhizal inoculation regimes. Biol Fertil Soil 33:351–357 Kaldrof M, Kuhn AH, Schroder WH, Hildebrandt U, Bothe H (1999) Selective element deposits in maize colonized by a heavy metal tolerance conferring arbuscular mycorrhizal fungus. J Plant Physiol 154:718–728 Kapoor R, Giri B, Mukerji KG (2002) Mycorrhization of coriander to enhance the concentration and quality of oil in seeds. J Sci Food Agric 82:1–4 Kapoor R, Giri B, Mukerji KG (2002a) Glomus macrocarpum: a potential bioinoculant to improve essential oil quality and concentration in dill (Anethum graveolens L.) and carum (Trachyspermum ammi (Linn.) Sprague). World J Microbiol Biotechnol 18:459–463 Kapoor R, Giri B, Mukerji KG (2004b) Improved growth and essential oil yield and quality in Foeniculum vulgare Mill on mycorrhizal inoculation supplemented with P-fertilizer. Bioresour Technol 93:309–311 Karagiannidis N, Hadjisawa-Zinoviadi S (1998) The mycorrhizal fungus Glomus mosseae enhances growth, yield and chemical composition of a durum wheat variety in 10 different soils. Nutr Cycl Agroecosys 55:1–7 Karagiannidis N, Nikolaou N (2000) Influence of arbuscular mycorrhizae on heavy metal (Pb and Cd) uptake, growth, and chemical composition of Vitis vinifera L.(cv. Razaki). Am J Enol Vitic 51:269–275 Kilham K, Firestone MK (1983) Vesicular-arbuscular mycorrhizal mediation of grass response to acidic and heavy metal depositions. Plant Soil 72:39–48 Koul M, Kapoor R, Luikham N (2001) Effect of lead contamination in soil on development of mycorrhiza in Cymopsis tetragonoloba (Linn.) Taub. Indian J Exp Biol 39:459–463 Kroopnik PM (1994) Vapor abatement cost analysis methodology for calculating life cycle costs for hydrocarbon vapor extracted during soil venting. In: Wise DL, Trantola DJ (eds) Remediation of hazardous waste. Marcel Dekker, New York, pp 779–790 Lakane E, Ervio R (1971) A comparison of eight extractants for the determination of plant available micronutrients on soil. Acta Agric Fenn 123:223–232 Leyval C, Turnau K, Hasclwandter K (1997) Effect of heavy metal pollution on mycorrhizal colonization and function: physiological, ecological and applied aspects. Mycorrhiza 7:139–153 Madalgeri MB, Shivakumar BG (2002) Cultivation practices of basella (Basella spp.) and celery (Apium graveolens L.). In: Govil JN (ed) Recent progress in medicinal plants: crop improvement production technology, trade and commerce. Science Technology Publishing, Houston, pp 165–174 Meharg AA, Cairney JWG (2000) Co-evolution of mycorrhizal symbionts and their hosts to metal-contaminated environments. Adv Ecol Res 30:69–112 Morgan JAW, Bending GD, White PJ (2005) Biological costs and benefits to plant-microbe interactions in the rhizosphere. J Exper Bot 56:1729–1739 Parker R (1994) Environmental restoration technologies. EMIAA Year book, pp 169–171 Phillips JH, Hayman DS (1970) Improved procedures for clearing roots and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infection. Trans Br Mycol Soc 55:158–161 Rivera-Becerril F, Calantzis C, Turnau K, Caussanel JP, Belimov A, Gianinazzi S, Gianinazzi-Pearson V (2002) Cadmium accumulation and buffering of cadmium-induced stress by arbuscular mycorrhiza in three Pisum sativum L. genotypes. J Exp Bot 53:1177–1185 Salim R, Al-Sbbu MM, Douleh A, Khalaf S (1992) Effects on growth and uptake of broad bean (Vicia faba L.) by root and foliar treatments of plant with lead and cadmium. J Environ Sci Health 27:1619–1642 Sharma MP, Gaur A, Bhatia NP, Adholeya A (1996) Growth responses and dependence of Acacia nilotica var. cupressiformis on the indigenous arbuscular mycorrhizal consortium of a marginal wasteland soil. Mycorrhiza 6:441–446 Sieverding E (1991) Vesicular-arbuscular mycorrhizal management in tropical agrosystem. Bremer Verlag, Germany Smith SE, Read DJ (1997) Mycorrhizal symbiosis. Academic, San Diego Srivastava D, Kapoor R, Srivastava SK, Mukerji KG (1996) Vesicular arbuscular mycorrhiza-an overview. In: Mukerji KG (ed) Concepts in mycorrhizal research. Kluwer, Netherlands, pp 1–39 Stobert AK, Grifith WT, Ameen-Bukhari I, Sherwood RP (1985) The effect of Cd on biosynthesis of chlorophyll in leaves of barley. Physiol Plant 63:293–298 Turnau K, Kottki I, Oberwinkler F (1993) Element localization in mycorrhizal roots of Pteridium aquilinum (L.) Kuhn collected from experimental plots treated with cadmium dust. New Phytol 123:313–324 Weissenhorn I, Glasshoff A, Leyval C, Berthlein J (1994) Differential tolerance to Cd and Zn of arbuscular mycorrhizal (AM) fungal spores isolated from heavy metal-polluted and unpolluted soils. Plant Soil 167:189–196 Wiessenhorn I, Leyval C, Belgy G, Berthelin J (1995) Arbuscular mycorrhizal contribution to heavy metal uptake by Maize (Zea mays L.) in pot culture with contaminated soil. Mycorrhiza 5:245–251 Williams GM (1988) Integrated studies into ground water pollution by hazardous wastes. In: Gronow JR, Schofield AN, Jain RK (eds) Land disposal of hazardous waste, engineering and environmental issues. Horwood, Chichester