Clonally Derived Human Embryonic Stem Cell Lines Maintain Pluripotency and Proliferative Potential for Prolonged Periods of Culture

Allsopp, 1992, Telomere length predicts replicative capacity of human fibroblasts, Proc. Natl. Acad. Sci. USA, 89, 10114, 10.1073/pnas.89.21.10114 Bodnar, 1998, Extension of life-span by introduction of telomerase into normal human cells, Science, 279, 349, 10.1126/science.279.5349.349 Chiu, 1996, Differential expression of telomerase activity in hematopoietic progenitors from adult human bone marrow, Stem Cells, 14, 239, 10.1002/stem.140239 Chiu, 1997, Replicative senescence and cell immortality: The role of telomeres and telomerase, Proc. Soc. Exp. Biol. Med., 214, 99, 10.3181/00379727-214-44075 Gearhart, 1998, New potential for human embryonic stem cells, Science, 282, 1061, 10.1126/science.282.5391.1061 Harley, 1990, Telomeres shorten during ageing of human fibroblasts, Nature, 345, 458, 10.1038/345458a0 Jiang, 1999, Telomerase expression in human somatic cells does not induce changes associated with a transformed phenotype, Nat. Genet., 21, 111, 10.1038/5056 Kaufman, 1992 Kim, 1994, Specific association of human telomerase activity with immortal cells and cancer, Science, 266, 2011, 10.1126/science.7605428 O'Rahilly, 1987 Price, P. J, Goldsborough, M. D, and, Tilkins, M. L. 1998, Embryonic stem cell serum replacement. International Patent Application WO98/30679. Reubinoff, 2000, Embryonic stem cell lines from human blastocysts: Somatic differentiation in vitro, Nat. Biotechnol., 18, 399, 10.1038/74447 Rossant, 1999, In search of the tabula rasa of human cells, Nat. Biotechnol., 17, 23, 10.1038/5194 Schrock, 1996, Multicolor spectral karyotyping of human chromosomes, Science, 273, 494, 10.1126/science.273.5274.494 Shamblott, 1998, Derivation of pluripotent stem cells from cultured human primordial germ cells, Proc. Natl. Acad. Sci. USA, 95, 13726, 10.1073/pnas.95.23.13726 Smith, 1988, Inhibition of pluripotential embryonic stem cell differentiation by purified polypeptides, Nature, 336, 688, 10.1038/336688a0 Thomson, 1998, Embryonic stem cell lines derived from human blastocysts, Science, 282, 1145, 10.1126/science.282.5391.1145 Thomson, 1995, Isolation of a primate embryonic stem cell line, Proc. Natl. Acad. Sci. USA, 92, 7844, 10.1073/pnas.92.17.7844 Thomson, 1998, Primate embryonic stem cells, Curr. Top. Dev. Biol., 38, 133, 10.1016/S0070-2153(08)60246-X Vaziri, 1994, Evidence for a mitotic clock in human hematopoietic stem cells: Loss of telomeric DNA with age, Proc. Natl. Acad. Sci. USA, 91, 9857, 10.1073/pnas.91.21.9857 Vaziri, 1993, Loss of telomeric DNA during aging of normal and trisomy 21 human lymphocytes, Am. J. Hum. Genet., 52, 661 Walz, 1995, Transforming growth factor-alpha (TGF-alpha) in human bone marrow: Demonstration of TGF-alpha in erythroblasts and eosinophilic precursor cells and of epidermal growth factor receptors in blastlike cells of myelomonocytic origin, Blood, 85, 2385, 10.1182/blood.V85.9.2385.bloodjournal8592385 Weinrich, 1997, Reconstitution of human telomerase with the template RNA component hTR and the catalytic protein subunit hTRT, Nat. Genet., 17, 498, 10.1038/ng1297-498 Williams, 1988, Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells, Nature, 336, 684, 10.1038/336684a0 Yang, 1999, Human endothelial cell life extension by telomerase expression, J. Biol. Chem., 274, 26141, 10.1074/jbc.274.37.26141