Isolation and characterization of endophytic plant growth-promoting bacteria from date palm tree (Phoenix dactylifera L.) and their potential role in salinity tolerance
Tóm tắt
Endophytic bacteria were isolated from date palm (Phoenix dactylifera L.) seedling roots, characterized and tested for their ability to help plants grow under saline conditions. Molecular characterization showed that the majority of these strains belonged to the genera Bacillus and Enterobacter and had different degrees of resistance to various antibiotics. Some of these strains were able to produce the enzyme 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase and the plant growth regulatory hormone indole-3-acetic acid (IAA). Some strains were also able to chelate ferric iron (Fe3+) and solubilize potassium (K+), phosphorus (PO
4
3-
) and zinc (Zn2+), and produce ammonia. The results also showed that ACC deaminase activity and IAA production was slightly increased in some strains in response to an increase in NaCl concentration in the growth media. Consistent with these results, selected strains such as PD-R6 (Paenibacillus xylanexedens) and PD-P6 (Enterobacter cloacae) were able to enhance canola root elongation when grown under normal and saline conditions as demonstrated by a gnotobiotic root elongation assay. These results suggest that the isolated and characterized endophytic bacteria can alter ethylene and IAA levels and also facilitate nutrient uptake in roots and therefore have the potential role to promote the growth and development of date palm trees growing under salinity stress.
Tài liệu tham khảo
Alam SM, Pessarakli M (1999) Nutrient uptake by plants under stress conditions. In: Pessarakli M (ed) Handbook of plant and crop stress, 2nd edn. Marcel Dekker, New York, pp 285–313
Alhammadi MS, Kurup SS (2012) Impact of salinity stress on date palm (Phoenix dactylifera L.)-a review, crop production technologies. In: P. Sharma (Ed.) InTech, 169–173
Ali S, Charles TC, Glick BR (2014) Amelioration of high salinity stress damage by plant growth-promoting bacterial endophytes that contain ACC deaminase. Plant Physiol Biochem 80:160–167
Basak B, Biswas D (2009) Influence of potassium solubilizing microorganism (Bacillus mucilaginosus) and waste mica on potassium uptake dynamics by sudan grass (Sorghum vulgare Pers.) grown under two Alfisols. Plant Soil 317:235–255
Bhore SJ, Ravichantar N, Loh CY (2010) Screening of endophytic bacteria isolated from leaves of Sambung Nyawa [Gynura procumbens (Lour.) Merr.] for cytokinin-like compounds. Bioinformation 5:191
Cheng Z, Park E, Glick BR (2007) 1-Aminocyclopropane-1-carboxylate deaminase from Pseudomonas putida UW4 facilitates the growth of canola in the presence of salt. Can J Microbiol 53:912–918
Duan J, Jiang W, Cheng Z, Heikkila JJ, Glick BR (2013) The complete genome sequence of the plant growth-promoting bacterium Pseudomonas sp. UW4. PLoS ONE 8:e58640
Duca D, Lorv J, Patten CL, Rose D, Glick BR (2014) Indole-3-acetic acid in plant–microbe interactions. Antonie van Leeuwenhoek 106:1–41
Dworkin M, Foster J (1958) Experiments with some microorganisms which utilize ethane and hydrogen. J Bacteriol 75:592
Faure D, Vereecke D, Leveau JH (2009) Molecular communication in the rhizosphere. Plant Soil 321:279–303
Gamalero E, Berta G, Glick B (2009) The use of microorganisms to facilitate the growth of plants in saline soils. In: Khan MS, Zaidi A, Musarrat J (eds) Microbial strategies for crop improvement. Springer, Berlin, pp 1–22
Glick BR (1995) The enhancement of plant growth by free-living bacteria. Can J Microbiol 41:109–117
Glick BR (2014) Bacteria with ACC deaminase can promote plant growth and help to feed the world. Microbiol Res 169:30–39
Glick BR, Penrose DM, Li J (1998) A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria. J Theor Biol 190:63–68
Glick BR, Cheng Z, Czarny J, Duan J (2007) Promotion of plant growth by ACC deaminase-producing soil bacteria. In: Bakker PAHM, Raaijmakers JM, Bloemberg G, Höfte M, Lemanceau L, Cooke BM (eds) New perspectives and approaches in plant growth-promoting rhizobacteria research. Springer, Heidelberg, pp 329–339
Glickmann E, Dessaux Y (1995) A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria. Appl Environ Microbiol 61:793–796
Gómez-Manzo S et al (2010) Molecular and catalytic properties of the aldehyde dehydrogenase of Gluconacetobacter diazotrophicus, a quinoheme protein containing pyrroloquinoline quinone, cytochrome b, and cytochrome c. J Bacteriol 192:5718–5724
Gordon SA, Weber RP (1951) Colorimetric estimation of indoleacetic acid. Plant Physiol 26:192
Gothwal R, Nigam V, Mohan M, Sasmal D, Ghosh P (2008) Screening of nitrogen fixers from rhizospheric bacterial isolates associated with important desert plants. Appl Ecol Env Res 6:101–109
Hariprasad P, Niranjana S (2009) Isolation and characterization of phosphate solubilizing rhizobacteria to improve plant health of tomato. Plant Soil 316:13–24
Hu X, Chen J, Guo J (2006) Two phosphate-and potassium-solubilizing bacteria isolated from Tianmu Mountain, Zhejiang, China. World J Microbiol Biotechnol 22:983–990
Iglesias MJ, Terrile MC, Bartoli CG, D’Ippólito S, Casalongué CA (2010) Auxin signaling participates in the adaptative response against oxidative stress and salinity by interacting with redox metabolism in Arabidopsis. Plant Mol Biol 74:215–222
Iglesias MJ, Terrile MC, Casalongué CA (2011) Auxin and salicylic acid signalling counteract during the adaptive response to stress. Plant Signal Behav 6:452–454
Indiragandhi P, Anandham R, Kim K, Yim W, Madhaiyan M, Sa T (2008) Induction of defense responses in tomato against Pseudomonas syringae pv. tomato by regulating the stress ethylene level with Methylobacterium oryzae CBMB20 containing 1-aminocyclopropane-1-carboxylate deaminase. World World J Microbiol Biotechnol 24:1037–1045
Iqbal U, Jamil N, Ali I, Hasnain S (2010) Effect of zinc-phosphate-solubilizing bacterial isolates on growth of Vigna radiata. Ann Microbiol 60:243–248
Jha B, Gontia I, Hartmann A (2012) The roots of the halophyte Salicornia brachiata are a source of new halotolerant diazotrophic bacteria with plant growth-promoting potential. Plant Soil 356:265–277
Kang S-M et al (2009) Gibberellin production and phosphate solubilization by newly isolated strain of Acinetobacter calcoaceticus and its effect on plant growth. Biotechnol Lett 31:277–281
Lane D (1991) 16S/23S rRNA sequencing. In: Stackebrandt Erko, Goodfellow Michael (eds) Nucleic acid techniques in bacterial systematics. Wiley, West Sussex, pp 125–175
Lichter A, Barash I, Valinsky L, Manulis S (1995) The genes involved in cytokinin biosynthesis in Erwinia herbicola pv. gypsophilae: characterization and role in gall formation. J Bacteriol 177:4457–4465
Marques AP, Pires C, Moreira H, Rangel AO, Castro PM (2010) Assessment of the plant growth promotion abilities of six bacterial isolates using Zea mays as indicator plant. Soil Biol Biochem 42:1229–1235
Mayak S, Tirosh T, Glick BR (2004a) Plant growth-promoting bacteria confer resistance in tomato plants to salt stress. Plant Physiol Biochem 42:565–572
Mayak S, Tirosh T, Glick BR (2004b) Plant growth-promoting bacteria that confer resistance to water stress in tomatoes and peppers. Plant Sci 166:525–530
Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681
Patten CL, Glick BR (2002) Role of Pseudomonas putida indoleacetic acid in development of the host plant root system. Appl Environ Microbiol 68:3795–3801
Peleg Z, Apse MP, Blumwald E (2011) Engineering salinity and water-stress tolerance in crop plants: getting closer to the field. Adv Bot Res 57:405–443
Penrose DM, Glick BR (2003) Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiol Plant 118:10–15
Piccoli P, Travaglia C, Cohen A, Sosa L, Cornejo P, Masuelli R, Bottini R (2011) An endophytic bacterium isolated from roots of the halophyte Prosopis strombulifera produces ABA, IAA, gibberellins A1 and A3 and jasmonic acid in chemically-defined culture medium. Plant Growth Regul 64:207–210
Pikovskaya RI (1948) Phosphate mobilization in soils as related to life processes of some microorganisms. Mikrobiologiya 17:362–370
Powell G, Morris R (1986) Nucleotide sequence and expression of a Pseudomonas savastanoi cytokinin biosynthetic gene: homotogy with Agrobacterium tumefaciens tmr and tzs loci. Nucleic Acids Res 14:2555–2565
Ramoliya P, Pandey A (2003) Soil salinity and water status affect growth of Phoenix dactylifera seedlings. New Zeal J Crop Hort 31:345–353
Rashid S, Charles TC, Glick BR (2012) Isolation and characterization of new plant growth-promoting bacterial endophytes. Appl Soil Ecol 61:217–224
Rosenblueth M, Martinez-Romero E (2006) Bacterial endophytes and their interactions with hosts. Mol Plant Microbe Interact 19:827–837
Ryan RP, Germaine K, Franks A, Ryan DJ, Dowling DN (2008) Bacterial endophytes: recent developments and applications. FEMS Microbiol Lett 278:1–9
Saleh SS, Glick BR (2001) Involvement of gacS and rpoS in enhancement of the plant growth-promoting capabilities of Enterobacter cloacae CAL2 and UW4. Can J Microbiol 47:698–705
Schulz B, Boyle C (2006) What are Endophytes? In: Schulz BE, Boyle CC, Sieber T (eds) Microbial Root Endophytes, vol 9., Soil BiologySpringer, Berlin, pp 1–13
Schwyn B, Neilands JB (1987) Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160:47–56
Sgroy V, Cassán F, Masciarelli O, Del Papa MF, Lagares A, Luna V (2009) Isolation and characterization of endophytic plant growth-promoting (PGPB) or stress homeostasis-regulating (PSHB) bacteria associated to the halophyte Prosopis strombulifera. Appl Microbiol Biotechnol 85:371–381
Siddikee M, Chauhan P, Anandham R, Han G-H, Sa T (2010) Isolation, characterization, and use for plant growth promotion under salt stress, of ACC deaminase-producing halotolerant bacteria derived from coastal soil. J Microbiol Biotechnol 20:1577–1584
Siddikee MA, Glick BR, Chauhan PS, Yim W, Sa T (2011) Enhancement of growth and salt tolerance of red pepper seedlings (Capsicum annuum L.) by regulating stress ethylene synthesis with halotolerant bacteria containing 1-aminocyclopropane-1-carboxylic acid deaminase activity. Plant Physiol Biochem 49:427–434
Sitbon F, Hennion S, Sundberg B, Little CA, Olsson O, Sandberg G (1992) Transgenic tobacco plants coexpressing the Agrobacterium tumefaciens iaaM and iaaH genes display altered growth and indoleacetic acid metabolism. Plant Physiol 99:1062–1069
Sugumaran P, Janarthanam B (2007) Solubilization of potassium containing minerals by bacteria and their effect on plant growth. World J Agric Sci 3:350–355
Talei D, Kadir MA, Yusop MK, Valdiani A, Abdullah MP (2012) Salinity effects on macro and micronutrients uptake in medicinal plant King of Bitters (Andrographis paniculata Nees.). Plant OMICS 5:271–278
Timmusk S, Paalme V, Pavlicek T, Bergquist J, Vangala A, Danilas T, Nevo E (2011) Bacterial distribution in the rhizosphere of wild barley under contrasting microclimates. PLoS ONE 6:e17968
Trobacher CP (2009) Ethylene and programmed cell death in plants. Botany 87:757–769
Wang Y, Brown H, Crowley D, Szaniszlo P (1993) Evidence for direct utilization of a siderophore, ferrioxamine B, in axenically grown cucumber. Plant Cell Environ 16:579–585
Wang C, Knill E, Glick BR, Défago G (2000) Effect of transferring 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase genes into Pseudomonas fluorescens strain CHA0 and its gac A derivative CHA96 on their growth-promoting and disease-suppressive capacities. Can J Microbiol 46:898–907
Wang Y, Li K, Li X (2009) Auxin redistribution modulates plastic development of root system architecture under salt stress in Arabidopsis thaliana. J Plant Physiol 166:1637–1645
Yamada T, Palm CJ, Brooks B, Kosuge T (1985) Nucleotide sequences of the Pseudomonas savastanoi indoleacetic acid genes show homology with Agrobacterium tumefaciens T-DNA. Proc Natl Acad Sci USA 82:6522–6526
Zahir ZA, Munir A, Asghar HN, Shaharoona B, Arshad M (2008) Effectiveness of rhizobacteria containing ACC deaminase for growth promotion of peas (Pisum sativum) under drought conditions. J Microbiol Biotechnol 18:958–963
Zahir ZA, Ghani U, Naveed M, Nadeem SM, Asghar HN (2009) Comparative effectiveness of Pseudomonas and Serratia sp. containing ACC-deaminase for improving growth and yield of wheat (Triticum aestivum L.) under salt-stressed conditions. Arch Microbiol 191:415–424
Zhu JK (2001) Plant salt tolerance. Trends Plant Sci 6:66–71
