Urease from Staphylococcus saprophyticus: purification, characterization and comparison to Staphylococcus xylosus urease

Archiv für Mikrobiologie - Tập 161 - Trang 393-399 - 1994
Udo Klaus Schäfer1, Heinrich Kaltwasser1
1Lehrstuhl für Mikrobiologie, Universität des Saarlandes, Saarbrücken, Germany

Tóm tắt

Urease from Staphylococcus saprophyticus was purified more than 800-fold by liquid chromatography reaching homogeneity, as shown by isoelectric focussing, at a maximum specific activity of 1979 U/mg. The molecular weight of the native enzyme was 420000; it consisted of subunits with molecular weights of 72400 (α), 20400 (β), and 13900 (γ) in an estimated (αβγ)4 stoichiometry. In native gradient polyacrylamide gel electrophoresis urease exhibited a multiple activity band pattern with molecular weights ranging from 420000 to 100000. In the native enzyme, 4.09 (±0.25) atoms of nickel per molecule were detected. The N-terminal amino acids of the urease subunits were identical to those from Staphylococcus xylosus, and amino acid analysis revealed high similarities in both enzymes; no cysteine was detected after acid hydrolysis of vinylpyridinylated urease. Electron micrographs of negatively stained urease specimens from both staphylococci showed identical size and structure.

Tài liệu tham khảo

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