Label-free cell separation and sorting in microfluidic systems

Springer Science and Business Media LLC - Tập 397 - Trang 3249-3267 - 2010
Daniel R. Gossett1,2, Westbrook M. Weaver1,2, Albert J. Mach1,2, Soojung Claire Hur2,3, Henry Tat Kwong Tse1,2, Wonhee Lee1,2, Hamed Amini1,2, Dino Di Carlo1,2
1Department of Bioengineering, University of California, Los Angeles, Los Angeles, USA
2California NanoSystems Institute, Los Angeles, USA
3Mechanical and Aerospace Engineering Department, University of California Los Angeles, Los Angeles, USA

Tóm tắt

Cell separation and sorting are essential steps in cell biology research and in many diagnostic and therapeutic methods. Recently, there has been interest in methods which avoid the use of biochemical labels; numerous intrinsic biomarkers have been explored to identify cells including size, electrical polarizability, and hydrodynamic properties. This review highlights microfluidic techniques used for label-free discrimination and fractionation of cell populations. Microfluidic systems have been adopted to precisely handle single cells and interface with other tools for biochemical analysis. We analyzed many of these techniques, detailing their mode of separation, while concentrating on recent developments and evaluating their prospects for application. Furthermore, this was done from a perspective where inertial effects are considered important and general performance metrics were proposed which would ease comparison of reported technologies. Lastly, we assess the current state of these technologies and suggest directions which may make them more accessible.

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