The <i>zntA</i> gene of <i>Escherichia coli</i> encodes a Zn(II)-translocating P-type ATPase

Proceedings of the National Academy of Sciences of the United States of America - Tập 94 Số 26 - Trang 14326-14331 - 1997
Christopher Rensing1,2,3, Bharati Mitra1,2,3, Barry P. Rosen1,2,3
1Cornell University, Ithaca, NY
2Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, 540 East Canfield Avenue, Detroit, MI 48201
3Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Scott Hall, 540 East Canfield Avenue, Detroit, MI 48201-1908.

Tóm tắt

The first Zn(II)-translocating P-type ATPase has been identified as the product of o732 , a potential gene identified in the sequencing of the Escherichia coli genome. This gene, termed zntA , was disrupted by insertion of a kanamycin gene through homologous recombination. The mutant strain exhibited hypersensitivity to zinc and cadmium salts but not salts of other metals, suggesting a role in zinc homeostasis in E. coli . Everted membrane vesicles from a wild-type strain accumulated 65 Zn(II) and 109 Cd(II) by using ATP as an energy source. Transport was sensitive to vanadate, an inhibitor of P-type ATPases. Membrane vesicles from the zntA ∷ kan strain did not accumulate those metal ions. Both the sensitive phenotype and transport defect of the mutant were complemented by expression of zntA on a plasmid.

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Proceedings of the National Academy of Sciences of the United States of America

Tập 94 Số 26

14326-14331

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