J-like protein family of Arabidopsis thaliana: the enigmatic cousins of J-domain proteins

Plant Cell Reports - Tập 41 - Trang 1343-1355 - 2022
Chetana Tamadaddi1,2, Amit K. Verma1,3, Vyankatesh Zambare4,5, Avanti Vairagkar1, Danish Diwan1,6, Chandan Sahi1,7
1Department of Biological Sciences, Indian Institute of Science Education and Research, Bhopal, India
2Department of Biology, Eberly College of Science, The Pennsylvania State University, University Park, USA
3Department of Biochemistry, University of Wisconsin-Madison, Madison, USA
4School of Biotechnology and Bioinformatics, D Y Patil Deemed to be University, Navi Mumbai, India
5Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, USA
6Department of Biology, University of Alabama, Birmingham, USA
7IISER Bhopal, Bhopal, India

Tóm tắt

J-like proteins (JLPs) are emerging as ancillaries to the cellular chaperone network. They modulate functions of Hsp70:J-domain protein (JDP) systems in novel ways thereby having key roles in diverse plant processes. J-domain proteins (JDPs) form an obligate co-chaperone partnership with Hsp70s with their highly conserved J-domain to steer protein quality control processes in the cell. The HPD motif between helix II and helix III of the J-domain is crucial for JDP’s interaction with Hsp70s. According to the most recent classification, J-like proteins (JLPs) form an extended class of the JDP family possessing a degenerate J-domain with the HPD motif non-conservatively replaced by other amino acid residues and hence are not able to interact with Hsp70s. Considering this most updated and acceptable JLP classification, we identified 21 JLPs in Arabidopsis thaliana that share a structurally conserved J-like domain (JLD), but lack the HPD motif. Analysis of publicly available gene expression data as well as real-time quantitative PCR performed for a few selected JLPs implicated some of these proteins in growth, development and stress response. Here, we summarize the current state of knowledge on plant JLPs and their involvement in vital plant cellular/metabolic processes, including chloroplast division, mitochondrial protein import and flowering. Finally, we propose possible modes of action for these highly elusive proteins and other DnaJ-related proteins (DNAJRs) in regulating the Hsp70 chaperone network.

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