Separation of jack bean (Canavalia ensiformis) urease isoforms by immobilized metal affinity chromatography and characterization of insecticidal properties unrelated to ureolytic activity

Plant Science - Tập 167 - Trang 241-246 - 2004
Cristian Follmer1, German E Wassermann1, Célia R Carlini1,2
1Graduate Program in Cellular and Molecular Biology, Center of Biotechnology, Universidade Federal do Rio Grande do Sul, Porto Alegre, CEP 91.501.970, RS, Brazil
2Department of Biophysics, IB, Universidade Federal do Rio Grande do Sul, Porto Alegre, CEP 91.501.970, RS, Brazil

Tài liệu tham khảo

Dixon, 1975, Jack bean urease (EC 3.5.1.5). A metalloenzyme. A simple biological role for nickel, J. Am. Chem. Soc., 97, 4131, 10.1021/ja00847a045 Mobley, 1989, Microbial ureases: significance, regulation and molecular characterization, Microbiol. Rev., 53, 85, 10.1128/MMBR.53.1.85-108.1989 Sumner, 1926, The isolation and crystallization of the enzyme urease, J. Biol. Chem., 69, 435, 10.1016/S0021-9258(18)84560-4 Hogan, 1983, Urease assay and ammonia release from leaf tissues, Phytochemistry, 22, 663, 10.1016/S0031-9422(00)86958-7 Polacco, 1982, Patterns of urease synthesis in developing soybeans, Plant Physiol., 70, 189, 10.1104/pp.70.1.189 Polacco, 1984, Soybean leaf urease: a seed enzyme, Plant Physiol., 74, 800, 10.1104/pp.74.4.800 Polacco, 1979, Comparisons of soybean urease isolated from seed and tissue culture, J. Biol. Chem., 254, 1707, 10.1016/S0021-9258(17)37830-4 Polacco, 1985, Structure and possible ureide degrading function of the ubiquitous urease of soybean, Plant Physiol., 79, 794, 10.1104/pp.79.3.794 Polacco, 1993, Roles of urease in plant cells, Int. Rev. Cytol., 145, 65, 10.1016/S0074-7696(08)60425-8 Carlini, 1981, Isolation and characterization of a toxic protein from Canavalia ensiformis (jack bean) seeds, distinct from concanavalin A, Toxicon, 19, 667, 10.1016/0041-0101(81)90104-5 Follmer, 2001, Canatoxin, a toxic protein from jack beans (Canavalia ensiformis), is a variant form of urease (EC 3.5.1.5): biological effects of urease independent of its ureolytic activity, Biochem. J., 360, 217, 10.1042/0264-6021:3600217 Carlini, 1985, Platelet release reaction and aggregation induced by canatoxin, a convulsant protein: evidence for the involvement of the platelet lipoxygenase pathway, Br. J. Pharmacol., 84, 551, 10.1111/j.1476-5381.1985.tb12940.x Ghazaleh, 1997, Stimulation of calcium influx and platelet activation by canatoxin: methoxyverapamil inhibition and downregulation by cGMP, Arch. Biochem. Biophys., 339, 362, 10.1006/abbi.1997.9898 Carlini, 1991, Plant and microbial toxic proteins as hemilectins: emphasis on canatoxin, Toxicon, 29, 791, 10.1016/0041-0101(91)90216-E Carlini, 1997, Biological effects of canatoxin in different insect models: evidence for a proteolytic activation of the toxin by insect cathepsinlike enzymes, J. Econ. Entomol., 90, 340, 10.1093/jee/90.2.340 Ferreira-DaSilva, 2000, Proteolytic activation of canatoxin, a plant toxic protein, by insect cathepsin-like enzymes, Arch. Insect Biochem. Physiol., 44, 162, 10.1002/1520-6327(200008)44:4<162::AID-ARCH3>3.0.CO;2-# Carlini, 2002, Plant toxic proteins with insecticidal properties. A review on their potentialities as bioinsecticides, Toxicon, 40, 1515, 10.1016/S0041-0101(02)00240-4 Blakeley, 1969, Jack bean urease (EC 3.5.1.5). A new purification and reliable rate assay, Biochemistry, 8, 1984, 10.1021/bi00833a031 Jabri, 1992, Preliminary crystallographic studies of urease from jack bean and from Klebsiella aerogenes, J. Mol. Biol., 227, 934, 10.1016/0022-2836(92)90232-9 Porath, 1992, Immobilized metal ion affinity chromatography, Protein Exp. Purif., 3, 263, 10.1016/1046-5928(92)90001-D Spector, 1978, Refinement of the Coomassie Blue method of protein quantitation. A simple and linear spectrophotometric assay for 0.5 to 50μg of protein, Anal. Biochem., 86, 142, 10.1016/0003-2697(78)90327-5 Weatherburn, 1967, Phenol-hypochlorite reaction for determination of ammonia, Anal. Chem., 39, 971, 10.1021/ac60252a045 Weber, 1969, The reliability of molecular weight determinations by dodecyl sulfate polyacrylamide gel electrophoresis, J. Biol. Chem., 244, 4406, 10.1016/S0021-9258(18)94333-4 Malmqvist, 1990, Quantitative PIXE analysis of biomedical material—sample preparation, Nucl. Instrum. Meth. Phys. Res., 49B, 183, 10.1016/0168-583X(90)90239-Q Mangelson, 1977, Proton induced X-ray emission analysis of biological samples: some approaches and applications, Nucl. Instrum. Meth. Phys. Res., 142, 133, 10.1016/0029-554X(77)90821-7 Campbell, 2000, The Guelph PIXE software package III: alternative proton database, Nucl. Instrum. Meth. Phys. Res., 170B, 193, 10.1016/S0168-583X(00)00156-7 Hu, 2002, Capillary electrophoresis for the analysis of biopolymers, Anal Chem., 74, 2833, 10.1021/ac0202379 Mamiya, 1985, Complete amino acid sequence of jack bean urease, Proc. Jpn. Acad., 61, 395, 10.2183/pjab.61.395 Pires-Alves, 2003, Characterization and expression of a novel member (JBURE-II) of the urease gene family from jackbean (Canavalia ensiformis DC), Plant Cell Physiol., 44, 139, 10.1093/pcp/pcg018 Zerner, 1991, Recent advances in the chemistry of an old enzyme, Urease. Bioorg. Chem., 19, 116, 10.1016/0045-2068(91)90048-T