Glyoxylate cycle in germination of Pinus laricio seeds: effects of phenolic compounds extracted from different forest soils
Tóm tắt
Low molecular weight phenolic compounds, identified in two differentsoils with vegetative cover, Fagus sylvatica L. andPinus laricio Poiret, spp. calabrica, were tested atdifferent concentrations on seed of Pinus laricio. Wedetermined the activity of catalase, responsible for degradation of fatty acidto provide substrates for gluconeogenesis and malate synthase,phosphoenolpyruvate carboxykinase and isocitrate lyase, enzymes of theglyoxylicpathway. The data obtained show that all the phenolic compounds bioassayedaffected seed germination inhibiting catalase and glyoxylic enzymes. Theresultsshow that the inhibition of Pinus laricio seed germinationby phenolic compounds is clearly linked to low utilisation of storage lipidswith a consequent deficit in glucose, the main respiratory substrate.
Tài liệu tham khảo
Aebi H.E. 1984. Catalase. In: Bergmeyer J. and Grassl M. (eds), Methods of Enzymatic Analysis. Verlag Chemie, Weinheim, pp. 273–282.
Bajracharya P. and Schopfer D. 1979. Effect of light on the development of glyoxysomal functions in the cotyledons of mustard (Sinapis alba L.) seedlings. Planta 145: 181–186.
Bogatek R., Zarska-Maciejewska B., Sinska I. and Lewak S. 1989. The embryonic axis controls lipid catabolism in cotyledons of apple seeds during germination. Physiol. Plant 76: 557–562.
Chell R.M., Sundaram T.K. and Wilkinson A.E. 1978. Isolation and characterization of isocitrate lyase from a thermophillic Bacillus sp. Biochem. J. 173: 165–177.
Ching T.M. 1972. Metabolism of germinating seeds. In: Kozlowski T.T. (ed.), Seed Biology. Academic Press, New York, pp. 103– 218.
Flaig W. 1971. Organic compounds in soil. Soil Sci. 111: 19–33.
Gerig T.M. and Blum U. 1991. Effects of mixture of four phenolic acids on leaf area expansion of cucumber seedlings grown in postsmoth B1 soil materials. J. Chem. Ecol. 17: 29–39.
Groome M.C., Axler S.R. and Gifford D.J. 1991. Hydrolysis of lipid and protein reserves in loblolly pine seeds in relation to protein electrophoretic patterns following imbibition. Physiol. Plant 83: 99–106.
Kaminsky R. and Muller W.H. 1977. The extraction of soil phytotoxins using a neutral EDTA solution. Soil. Sci. 124: 205–210.
Kuiters A.T. and Sarink H.M. 1986. Leaching of phenolic compounds from leaf and needle litter of several deciduous and coniferous trees. Soil. Biol. Biochem. 18: 475–480.
Leather G.R. and Einhelling F.A. 1988. Bioassay of naturally occurring allelochemicals for toxicity. J. Chem. Ecol. 14: 1821– 18.
Leegod R.C. and ap Rees T. 1978. Phosphoenolpyruvate carboxykinase and gluconeogenesis in cotyledons of Cucurbita pepo. Biochem. Biophys. Acta. 524: 207–218.
Li H.-H., Inove M., Nishimura H., Mizutani J. and Tsuzuki E. 1993. Interactions of trans-cinnamic acid, its related phenolics allelochemicals and abscisic acid in seedling growth and seed germination of lettuce. J. Chem. Ecol. 19: 1775–1787.
Meletiou-Christou M.S., Diamantoglou S. and Mitrakos K. 1990. Analysis of lipids of Citrus lanatus (cv. Sugar baby) during seed germination and seedling growth. J. Exp. Bot. 41: 1455– 1460.
Mullen R.T. and Gifford D.J. 1995. Purification and characterization of the glyoxisomal enzyme malate synthase following seed germination in Pinus taeda. Plant Phisiol. Biochem. 33: 639– 648.
Muscolo A., Panuccio M.R. and Sidari M. 2001. Respiratory enzyme activities during germination of Pinus laricio seeds treated with phenols extracted from different forest soils. Plant Growth Regul. 35: 31–35.
Rasmussen J.A. and Einhellig F.A. 1997. Synergistic effects of pcoumaric and ferulic cidson germination and growth of sorghum. J. Chem. Ecol. 3: 197–207.
Reigosa M.J., Souto X.C. and Gonzales L. 1999. Effect of phenolic compounds on the germination of six weeds species. Plant Growth Regul. 28: 83–88.
Walker R.P. and Leegod R.C. 1995. Purification, and phosphorilation in vivo and in vitro, of phosphoenol-piruvate carboxykinase from cucumber cotyledons. FEBS Lett. 362: 70–74.
Walker R.P., Trevanion S.J. and Leegod R.C. 1995. Phosphoenolpyruvate carboxykinase from higher plants: Purification from cucumber and evidence of rapid proteolytic cleavage in extracts from a range of plant tissues. Planta 196: 58–63.
Whitehead D.C., Dibb H. and Hartley R.D. 1982. Phenolic compounds in soil as influenced by the growth of different plant species. J. of Appl. Ecol. 19: 529–538.
Williamson G.B. 1990. Allelopathy, Koch’s postulates and the neck riddle. In: Grace and Tilman (eds), Perspective on Plant Competition. Academic Press, San Diego, pp. 143–162.
Wingler A., Walker R.P., Chen Z.-H. and Leegod R.C. 1999. Phosphoenolpyruvate carboxykinase is involved in the decarboxylation of aspartate in the bundle sheath of maize. Plant Physiol. 120: 539–545.