Middle-aged rat hippocampus and some early changes accompanying aging

Central European Journal of Biology - Tập 7 - Trang 810-816 - 2012
Zdenka Gasparova1, Pavol Janega2,3, Nada Pronayova4, Tibor Liptaj4
1Department of Neuropharmacology, Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovak Republic
2Department of Pathology, Faculty of Medicine, Comenius University, Bratislava, Slovak Republic
3Institute of Normal and Pathological Physiology, Slovak Academy of Sciences, Bratislava, Slovak Republic
4Department of NMR Spectroscopy, Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovak Republic

Tóm tắt

Studies of the middle-aged hippocampus may clarify the early mechanisms of aging and may lead to timely initiation of treatment for age-related cognitive impairments. Young (2-m/o) and middle aged (15- and 17-m/o) rats were used with the aim to characterize early differences in electrophysiological properties, morphometrical changes and 1H-nuclear magnetic resonance (NMR) metabolite content in the hippocampus. A decrease of the neuronal number accompanied by reduced width of the CA1 pyramidal cell layer was observed in middle-aged rats which may correspond to their reduced neurotransmission found in the CA3-CA1 synapse. An absolute value of mean magnitude of baseline field potential as well as of response obtained 51–60 min after high frequency stimulation was significantly lower in the middle-aged rats. The measurement of 1H-NMR metabolite content in hippocampal extracts of 2- and 17-m/o rats revealed that none of the sixteen metabolites identified can serve as a marker for middle-age-related changes. Functional and morphological changes are observed in middle-aged hippocampus. Therefore, 15–17-m/o rats could serve as proper animal model in studies focused on aging. Further studies aimed at timely prevention of early changes in the brain may contribute in slowing down of physiological brain aging.

Tài liệu tham khảo

Suzuki Y., Takagi Y., Nakamura R., Hashimoto K., Umemura K., Ability of NMDA and non-NMDA receptor antagonists to inhibit cerebral ischemic damage in aged rats, Brain Res., 2003, 964, 116–120 McCutcheon J.E., Marinelli M., Age matters, Eur. J. Neurosci., 2009, 29, 997–1014 Lynch M.A., Analysis of the mechanisms underlying the age-related impairment in long-term potentiation in the rat, Rev. Neurosci., 1998, 9, 169–201 Foster T.C., Kumar A., Susceptibility to induction of long-term depression is associated with impaired memory in aged Fischer 344 rats, Neurobiol. Learn. Mem., 2007, 87, 522–535 Lister J.P., Barnes C.A, Neurobiological changes in the hippocampus during normative aging, Arch. Neurol., 2009, 66, 829–833 Kumar A., Long-term potentiation at CA3-CA1 hippocampal synapses with special emphasis on aging, disease, and stress, Front Ag Neurosci., 2011, 3, 7–27 Lamour Y., Bassant M.H., Potier B., Billard J.M., Dutar P., Aging of memory mechanisms, C. R. Seances Soc. Biol. Fil., 1994, 188, 469–486 De Toledo-Morrell L., Geinisman Y., Morrell F., Age dependent alterations in hippocampal synaptic plasticity in relation to memory disorders, Neurobiol. Aging, 1988, 9, 581–590 Eriksdotter-Nilsson M., Gerhardt G., Seiger A., Olson L., Hoffer B., Granholm A.C., Age — related alterations in noradrenergic input to the hippocampal formation: structural and functional studies in intraocular transplants, Brain Res., 1989, 478, 269–280 Fischer W., Gage F.H., Bjorklund A., Degenerative changes in forebrain cholinergic nuclei corelate with cognitive impairments in aged rats, Eur. J. Neurosci., 1989, 1, 34–45 West M.J., Kawas C.H., Stewart W.F., Rudiw G.L., Troncoso J.C., Hippocampal neurons in pre-clinical Alzheimer’s disease, Neurobiol. Aging, 2004, 25, 1205–1212 Lucassen P.J., Heine V.M., Muller M.B., van der Beek E.M., Wiegant V.M., De Kloet E.R., et al., Stress, depression and hippocampal apoptosis, CNS Neurol. Disord. Drug Targets, 2006, 5, 531–546 Mueller S.G., Schuff N., Yaffe K., Madison C., Miller B., Weiner M.V., Hippocampal atrophy paterns in mild cognitive impairment and Alzheimer’s disease, Hum. Brain Mapp., 2010, 31, 1339–1247 Rigotti D.J., Inglese M., Gonen O., Whole-brain N-acetylaspartate as a surrogate marker of neuronal damage in diffuse neurologic disorders, Am. J. Neuroradiol., 2007, 28, 1843–1849 Abramoff M.D., Magalhaes P.J., Ram S.J., Image Processing with ImageJ, Biophotonics Internat., 2004, 11, 36–42 Macri M.A., D’Alessandro N., Di Giulio C., Di Iorio P., Di Luzio S., Giuliani P., et al., Regional changes in metabolite profile after long-term hypoxia-ischemia in brains of young and aged rats: A quantitative proton MRS study, Neurobiol. Aging, 2006, 27, 98–104 Bradford M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 1976, 72, 248–254 Barnes C.A., McNaughton B.L., Physiological compensation for loss of afferent synapses in rat hippocampal granule cells during senescence, J. Physiol., 1980, 309, 473–485 Levkovitz Y., Richter-Levin G., Segal M., Effect of 5-hydroxytyptophane on behavior and hippocampal physiology in young and old rats, Neurobiol. Aging, 1994, 15, 635–641 Papatheodoropoulos C., Kostopoulos G., Agerelated changes in excitability and recurrent inhibition in the CA1 hippocampal region, Eur. J. Neurosci., 1996, 8, 510–520 Kadar T., Silbermann M., Brandeis R., Levy A., Agerelated structural changes in the rat hippocampus: correlation with working memory deficiency, Brain Res., 1990, 512, 113–120 Is M., Comunoglu N.U., Comunoglu C., Eren B., Ekici I.D., Ozkan F., Age-related changes in the rat hippocampus, J. Clin. Neurosci., 2008, 15, 68–574 Schuff N., Amend D.L., Knowlton R., Norman D., Fein G., Weiner M.W., Age-related metabolite changes and volume loss in the hippocampus by magnetic resonance spectroscopy and imaging, Neurobiol, Aging, 1999, 20, 279–285 Kumar A., Thinschmidt J.S., Foster T.C., King M.A., Aging effects on the limits and stability of long-term synaptic potentiation and depression in rat hippocampal area CA1, J. Neurophysiol., 2007, 98, 594–601 Christiansen P., Toft P., Larsson H.B., Stubgaard M., Henriksen O., The concentration of N-acetyl aspartate, creatine + phosphocreatine, and choline in different parts of the brain in adulthood and senium, Magn. Reson. Imaging., 1993, 11, 799–806 Charles H.C., Lazevras F., Krishnan K.R., Buyko O.B., Patterson L.J., Doraiswamy P.M., et al., Proton spectroscopy of human brain: effects of age and sex, Prog. Neuropsychopharmacol. Biol. Psychiatry, 1994, 18, 995–1004 Pfefferbaum A., Adalsteinsson E., Spielman D., Sullivan E.V., Lim K.O., In vivo brain concentrations of N-acetyl compounds, creatine, and choline in Alzheimer disease, Gen. Psychiatry, 1999, 56, 185–192 Saunders D.E., Howe F.A., van den Boogaart A., Griffiths J.R., Brown M.M., Aging of the adult human brain: in vivo quantitation of metabolite content with proton magnetic resonance spectroscopy, J. Magn. Reson. Imaging, 1999, 9, 711–716 Reyngoudt H., Claeys T., Vlerick L., Verleden S., Acou M., Deblaere K., et al., Age-related differences in metabolites in the posterior cingulate cortex and hippocampus of normal aging brain: A (1)H-MRS study, Eur. J. Radiol., 2012, 81, 223–231 Zhang X., Liu H., Wu J., Zhang X., Liu M., Wang Y., Metabonomic alterations in hippocampus, temporal and prefrontal cortex with age in rats, Neurochem. Int. 2009, 54, 481–487 Paban V., Fauvelle F., Alescio-Lautier B., Age-related changes in metabolic profiles of rat hippocampus and cortices, Eur. J. Neurosci., 2010, 31, 1063–1073