Arp2/3 Is Critical for Lamellipodia and Response to Extracellular Matrix Cues but Is Dispensable for Chemotaxis

Allen, 2007, Imaging of germinal center selection events during affinity maturation, Science, 315, 528, 10.1126/science.1136736 Arrieumerlou, 2005, A local coupling model and compass parameter for eukaryotic chemotaxis, Dev. Cell, 8, 215, 10.1016/j.devcel.2004.12.007 Bear, 2002, Antagonism between Ena/VASP proteins and actin filament capping regulates fibroblast motility, Cell, 109, 509, 10.1016/S0092-8674(02)00731-6 Berginski, 2011, High-resolution quantification of focal adhesion spatiotemporal dynamics in living cells, PLoS ONE, 6, e22025, 10.1371/journal.pone.0022025 Cai, 2007, Coronin 1B coordinates Arp2/3 complex and cofilin activities at the leading edge, Cell, 128, 915, 10.1016/j.cell.2007.01.031 Cai, 2008, Coronin 1B antagonizes cortactin and remodels Arp2/3-containing actin branches in lamellipodia, Cell, 134, 828, 10.1016/j.cell.2008.06.054 Condeelis, 2005, The great escape: when cancer cells hijack the genes for chemotaxis and motility, Annu. Rev. Cell Dev. Biol., 21, 695, 10.1146/annurev.cellbio.21.122303.120306 Di Nardo, 2005, Arp2/3 complex-deficient mouse fibroblasts are viable and have normal leading-edge actin structure and function, Proc. Natl. Acad. Sci. USA, 102, 16263, 10.1073/pnas.0508228102 DiMilla, 1993, Maximal migration of human smooth muscle cells on fibronectin and type IV collagen occurs at an intermediate attachment strength, J. Cell Biol., 122, 729, 10.1083/jcb.122.3.729 Goeckeler, 2008, Nonmuscle myosin II is responsible for maintaining endothelial cell basal tone and stress fiber integrity, Am. J. Physiol. Cell Physiol., 295, C994, 10.1152/ajpcell.00318.2008 Goley, 2006, The ARP2/3 complex: an actin nucleator comes of age, Nat. Rev. Mol. Cell Biol., 7, 713, 10.1038/nrm2026 Gupton, 2006, Spatiotemporal feedback between actomyosin and focal-adhesion systems optimizes rapid cell migration, Cell, 125, 1361, 10.1016/j.cell.2006.05.029 Hoeller, 2007, Chemotaxis in the absence of PIP3 gradients, Curr. Biol., 17, 813, 10.1016/j.cub.2007.04.004 Kamijo, 1997, Tumor suppression at the mouse INK4a locus mediated by the alternative reading frame product p19ARF, Cell, 91, 649, 10.1016/S0092-8674(00)80452-3 Kim, 2006, The regulation of INK4/ARF in cancer and aging, Cell, 127, 265, 10.1016/j.cell.2006.10.003 Melvin, 2011, In chemotaxing fibroblasts, both high-fidelity and weakly biased cell movements track the localization of PI3K signaling, Biophys. J., 100, 1893, 10.1016/j.bpj.2011.02.047 Monypenny, 2009, Cdc42 and Rac family GTPases regulate mode and speed but not direction of primary fibroblast migration during platelet-derived growth factor-dependent chemotaxis, Mol. Cell. Biol., 29, 2730, 10.1128/MCB.01285-08 Mullins, 1998, The interaction of Arp2/3 complex with actin: nucleation, high affinity pointed end capping, and formation of branching networks of filaments, Proc. Natl. Acad. Sci. USA, 95, 6181, 10.1073/pnas.95.11.6181 Nicholson-Dykstra, 2008, Arp2 depletion inhibits sheet-like protrusions but not linear protrusions of fibroblasts and lymphocytes, Cell Motil. Cytoskeleton, 65, 904, 10.1002/cm.20312 Nolen, 2009, Characterization of two classes of small molecule inhibitors of Arp2/3 complex, Nature, 460, 1031, 10.1038/nature08231 Ozgur, 2011, The Kaposi's sarcoma-associated herpesvirus ORF6 DNA binding protein forms long DNA-free helical protein filaments, J. Struct. Biol., 174, 37, 10.1016/j.jsb.2010.10.015 Parent, 2004, Making all the right moves: chemotaxis in neutrophils and Dictyostelium, Curr. Opin. Cell Biol., 16, 4, 10.1016/j.ceb.2003.11.008 Pollard, 2003, Cellular motility driven by assembly and disassembly of actin filaments, Cell, 112, 453, 10.1016/S0092-8674(03)00120-X Pollard, 2009, Actin, a central player in cell shape and movement, Science, 326, 1208, 10.1126/science.1175862 Rogers, 2003, Molecular requirements for actin-based lamella formation in Drosophila S2 cells, J. Cell Biol., 162, 1079, 10.1083/jcb.200303023 Schwob, 1992, New yeast actin-like gene required late in the cell cycle, Nature, 355, 179, 10.1038/355179a0 Shamloo, 2008, Endothelial cell polarization and chemotaxis in a microfluidic device, Lab Chip, 8, 1292, 10.1039/b719788h Steffen, 2006, Filopodia formation in the absence of functional WAVE- and Arp2/3-complexes, Mol. Biol. Cell, 17, 2581, 10.1091/mbc.e05-11-1088 Svitkina, 1999, Arp2/3 complex and actin depolymerizing factor/cofilin in dendritic organization and treadmilling of actin filament array in lamellipodia, J. Cell Biol., 145, 1009, 10.1083/jcb.145.5.1009 Swaney, 2010, Eukaryotic chemotaxis: a network of signaling pathways controls motility, directional sensing, and polarity, Annu Rev Biophys, 39, 265, 10.1146/annurev.biophys.093008.131228 Thiery, 1984, Mechanisms of cell migration in the vertebrate embryo, Cell Differ., 15, 1, 10.1016/0045-6039(84)90024-1 Urban, 2010, Electron tomography reveals unbranched networks of actin filaments in lamellipodia, Nat. Cell Biol., 12, 429, 10.1038/ncb2044 Vitriol, 2007, Enhanced EGFP-chromophore-assisted laser inactivation using deficient cells rescued with functional EGFP-fusion proteins, Proc. Natl. Acad. Sci. USA, 104, 6702, 10.1073/pnas.0701801104 Webb, 2002, Adhesion assembly, disassembly and turnover in migrating cells — over and over and over again, Nat. Cell Biol., 4, E97, 10.1038/ncb0402-e97 Wickramarachchi, 2010, Immune pathology associated with altered actin cytoskeleton regulation, Autoimmunity, 43, 64, 10.3109/08916930903374634 Wynn, 2008, Cellular and molecular mechanisms of fibrosis, J. Pathol., 214, 199, 10.1002/path.2277 Yae, 2006, Sleeping beauty transposon-based phenotypic analysis of mice: lack of Arpc3 results in defective trophoblast outgrowth, Mol. Cell. Biol., 26, 6185, 10.1128/MCB.00018-06 Zaki, 2007, Replacement of the essential Dictyostelium Arp2 gene by its Entamoeba homologue using parasexual genetics, BMC Genet., 8, 28, 10.1186/1471-2156-8-28 Bear, J.E., Loureiro, J.J., Libova, I., Fässler, R., Wehland, J., and Gertler, F.B. (2000). Negative regulation of fibroblast motility by Ena/VASP proteins. Cell 101, 717–728. Bear, J.E., Svitkina, T.M., Krause, M., Schafer, D.A., Loureiro, J.J., Strasser, G.A., Maly, I.V., Chaga, O.Y., Cooper, J.A., Borisy, G.G., and Gertler, F.B. (2002). Antagonism between Ena/VASP proteins and actin filament capping regulates fibroblast motility. Cell 109, 509–521. Berginski, M.E., Vitriol, E.A., Hahn, K.M., and Gomez, S.M. (2011). High-resolution quantification of focal adhesion spatiotemporal dynamics in living cells. PLoS ONE 6, e22025. Cai, L., Makhov, A.M., Schafer, D.A., and Bear, J.E. (2008). Coronin 1B antagonizes cortactin and remodels Arp2/3-containing actin branches in lamellipodia. Cell 134, 828–842. Cai, L., Marshall, T.W., Uetrecht, A.C., Schafer, D.A., and Bear, J.E. (2007). Coronin 1B coordinates Arp2/3 complex and cofilin activities at the leading edge. Cell 128, 915–929. Goeckeler, Z.M., Bridgman, P.C., and Wysolmerski, R.B. (2008). Nonmuscle myosin II is responsible for maintaining endothelial cell basal tone and stress fiber integrity. Am. J. Physiol. Cell Physiol. 295, C994–C1006. Isaji, T., Sato, Y., Fukuda, T., and Gu, J. (2009). N-glycosylation of the I-like domain of beta1 integrin is essential for beta1 integrin expression and biological function: identification of the minimal N-glycosylation requirement for alpha5beta1. J. Biol. Chem. 284, 12207–12216. Katsumoto, T., Naguro, T., Iino, A., and Takagi, A. (1981). The effect of tannic acid on the preservation of tissue culture cells for scanning electron microscopy. J. Electron Microsc. (Tokyo) 30, 177–182. Ozgur, S., Damania, B., and Griffith, J. (2011). The Kaposi's sarcoma-associated herpesvirus ORF6 DNA binding protein forms long DNA-free helical protein filaments. J. Struct. Biol. 174, 37–43. Svitkina, T.M., and Borisy, G.G. (2005). Correlative light and electron microscopy studies of the cytoskeletal dynamics. In Cell Biology: A Laboratory Handbook, J. Celis, ed. (Burlington, MA: Academic Press), 277–286.