Cytotoxic and immunopotentiating effects of ethanolic extract of Nigella sativa L. seeds

Agarwal, 1949, Antimicrobial and antihelminthic activity of the essential oil of Nigella sativa Linn, Indian Journal of Experimental Biology, 17, 1264 Atta-ur-Raman, Sohail Malik, Sadiq Hasan, S., Iqbal Choudhary, M., Chao-Zhou Ni and Jon Clardy, 1995. Nigellidine—A new indazole alkaloid from the seeds of Nigella sativa. Tetrahedron Letters 36 (12), 1993–1996. Atta-ur-Raman, Sohail Malik and Khurshid Zaman, 1992. Nigellimine: a new isoquinoline alkaloid from the seeds of Nigella sativa. Journal of Natural Products 55 (5), 676–678. Atta-ur-Raman and Sohail Malik 1985. Isolation and Structure determination of Nigellicine, a novel alkaloid from the seeds of Nigella sativa. Tetrahedron Letters 26 (23), 2759–2762. Boulos, L., 1983. Medicinal Plants of North Africa, Reference Publication, Algonac, Michigan, p. 103. Chakravarty, 1993, Inhibition of histamine release from mast cells by nigellone, Annals Allergy, 70, 237 David R. Worthen, Omar A Ghosheh and Peter A.C., 1998, The in-vitro anti-tumor activity of some crude and purified components of Blackseed, Nigella sativa L. Anticancer Research 18, 1527–1532. Geran, R.I., Greenberg, N.H., Macdonald, M.M., Schumacher, A.M., Abbott, B.J., 1972. Protocols for screening chemical agents and natural products against animal tumors and other biological systems. Cancer Chemotherapy Reports Part 3, 3(2), 1–17, 59–61. Gerlier, 1986, Use of MTT colorimetric assay to measure cell activation, Journal of Immunological Methods, 94, 57, 10.1016/0022-1759(86)90215-2 Houghton, 1995, Fixed oil of Nigella sativa and derived thymoquinone inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation, Planta Medica, 61, 33, 10.1055/s-2006-957994 Mosmann, 1983, Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, Journal of Immunological Methods, 65, 55, 10.1016/0022-1759(83)90303-4 Nakamura, 1986, A novel method of screening for immunomodulating substances, establishment of an assay system and its application to culture broths of microorganisms, Journal of Antibiotics, 39, 1148, 10.7164/antibiotics.39.1148 Nair, 1991, Modulatory effects of Crocus sativus and Nigella sativa extracts on cisplatin-induced toxicity in mice, Journal of Ethnopharmacology, 31, 75, 10.1016/0378-8741(91)90146-5 Salomi, 1989, Cytotoxic action of Nigella sativa seeds, Proceedings of Keralite Science Congress, 11, 202 Salomi, 1991, Inhibitory effects of Nigella sativa and saffron (Crocus sativus) on chemical carcinogenesis in mice, Nutrition Cancer, 16, 67, 10.1080/01635589109514142 Salomi, 1992, Antitumor principles from Nigella sativa seeds, Cancer Letters, 63, 41, 10.1016/0304-3835(92)90087-C Scudiero, 1988, Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines, Cancer Research, 48, 4827 Wu, R.T., Chiang, H.C., Fu, W.C., Chien, K.Y., Chung, Y.M., Horng, L.Y., 1990. Formosanin C, an immunomodulator with antitumor activity. International Journal of Immunopharmacology 12, 777–786.