Successful fertilization, embryo development, and pregnancy in human in vitro fertilization (IVF) using a chemically defined culture medium containing no protein
Tóm tắt
A randomized control trial involving the fertilization and culture of human embryos in culture medium (T6) conraining either 10% maternal serum or no protein or amino acid supplement was carried out to assess the effect of deletion from culture of all fixed nitrogen on fertilization, embryo development, and embryo viability. There was no difference in fertilization rates (68 vs 69%), development of apparently normal embryos (96 vs 97%), pregnancy rate (18 vs 14%), or birth rate (13 vs 11%) between protein-containing and protein-free media. Deletion of protein from the culture medium may enable the constitution of more appropriate and defined culture media for human in vitro fertilization (IVF).
Tài liệu tham khảo
Cholewa JA, Whitten WK: Development of 2-cell mouse embryos in vitro. III. The effect of fixed nitrogen source. J Exp Zool 1965;158:69–78
Caro CM, Trounson A: The effect of protein on preimplantation mouse embryo development in vitro. J Vitro Fert Embryo Transfer 1984;1:183–187
Shirley B, Wortham JWE, Witmyer J, Condon-Mahony M, Fort G: Effects of human serum and plasma on development of mouse embryos in culture medium. Fertil Steril 1985;43:129–134
Trounson A, Calabrese R: Changes in plasma progesterone concentrations around the time of the Luteinizing Hormone surge in women superovulated for in vitro fertilization. J Clin Endocrinol Metab 1984;59:1075–1080
Trounson AO, Mohr LR, Wood C, Leeton JF: Effect of delayed insemination on in vitro fertilization, culture and transfer of human embryos. J Reprod Fert 1982;64:285–294
Mahadevan M, Trounson AO: The influence of seminal characteristics on the success rate of human in vitro fertilization. Fertil Steril 1984;42:400–405
Leeton J, Trounson A, Jessup D, Wood C: The technique of human embryo transfer. Fertil Steril 1982;38:156–160
Menezo Y, Testart J, Perrone D: Serum is not necessary for human in vitro fertilization, early embryo culture and transfer. Fertil Steril 1984;42:750–755
Trounson A: In vitro fertilization and embryo preservation.In In Vitro Fertilization and Embryo Transfer, A Trounson, C Wood (eds). Edinburgh, Churchill Livingstone 1984, pp 111–130