Carbon-limited fed-batch production of medium-chain-length polyhydroxyalkanoates by aphaZ-knockout strain ofPseudomonas putidaKT2440

Oxford University Press (OUP) - Tập 42 Số 4 - Trang 637-646 - 2015
Minh Tri Vo1, Kenton Ko2, Bruce A. Ramsay3,1
1grid.410356.5 0000000419368331 Chemical Engineering Queen’s University K7L 3N6 Kingston ON Canada
2grid.410356.5 0000000419368331 Biology Queen’s University 116 Barrie St. K7L 3N6 Kingston ON Canada
3Polyferm Canada 4530 Camden-Portland Bdry Rd, RR#1 K0H 1V0 Harrowsmith ON Canada

Tóm tắt

AbstractA medium-chain-length poly-3-hydroxyalkanote (MCL-PHA) depolymerase knockout mutant of Pseudomonas putida KT2440 was produced by double homologous recombination. A carbon-limited shake-flask study confirmed that depolymerase activity was eliminated. Lysis of both mutant and wild-type strains occurred under these conditions. In carbon-limited, fed-batch culture, the yield of unsaturated monomers from unsaturated substrate averaged only 0.62 mol mol−1 for the phaZ minus strain compared to 0.72 mol mol−1 for the wild type. The mutant strain also produced more CO2 and less residual biomass from the same amount of carbon substrate. However, most results indicated that elimination of PHA depolymerase activity had little impact on the overall yield of biomass and PHA.

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