Wen Liu1, Joachim Ahlert1, Qunjie Gao1, Evelyn Wendt-Pienkowski1, Ben Shen1, Jon S. Thorson1
1Pharmaceutical Sciences Division, School of Pharmacy, Laboratory for Biosynthetic Chemistry, and Department of Chemistry, University of Wisconsin, 777 Highland Avenue, Madison, WI 53705
Tóm tắt
A universal PCR method for the rapid amplification of minimal enediyne polyketide synthase (PKS) genes and the application of this methodology to clone remaining prototypical genes from producers of structurally determined enediynes in both family types are presented. A phylogenetic analysis of the new pool of bona fide enediyne PKS genes, consisting of three from 9-membered producers (neocarzinostatin, C1027, and maduropeptin) and three from 10-membered producers (calicheamicin, dynemicin, and esperamicin), reveals a clear genotypic distinction between the two structural families from which to form a predictive model. The results from this study support the postulation that the minimal enediyne PKS helps define the structural divergence of the enediyne core and provides the key tools for generating enediyne hybrid genes/molecular scaffolds; by using the model, a classification is also provided for the unknown enediyne PKS genes previously identified via genome scanning.
