Identification of anisakid nematodes with zoonotic potential from Europe and China by single-strand conformation polymorphism analysis of nuclear ribosomal DNA
Tóm tắt
Using genetic markers defined previously in the second internal transcribed spacer (ITS-2) of nuclear ribosomal DNA (rDNA), isotopic, and non-isotopic polymerase-chain-reaction-coupled single-strand conformation polymorphism (SSCP) were utilized to identify each of three anisakid species [Anisakis simplex (s.l.), Contracaecum
osculatum (s.l.), and Hysterothylacium aduncum] from different host species and geographical locations in Poland and Sweden. While subtle microheterogeneity was observed within each of Anisakis simplex (s.l.) and H. aduncum, distinct SSCP profiles were displayed for each of the three species, allowing identification and differentiation of the three taxa. Subsequent sequencing of the ITS-1 and ITS-2 rDNA revealed that A. simplex (s.l.) represented Anisakis simplex s.s. and Contracaecum osculatum (s.l.) represented C. osculatum C. Application of the non-isotopic SSCP assay of ITS-2 to larval anisakid samples from different hosts and geographical locations in China revealed three distinct SSCP profiles, one of which was consistent with that of A. simplex (s.l.), and the other two had different SSCP profiles from that of C. osculatum C and H. aduncum. Sequencing of the ITS-1 and ITS-2 rDNA for representative Chinese anisakid samples examined revealed three anisakid species in China, i.e., Anisakis typica, Anisakis pegreffii, and Hysterothylacium sp. These molecular tools will be useful for identification and investigation of the ecology of anisakid nematodes in China and elsewhere.
Tài liệu tham khảo
Adams AM, Murrell KD, Cross JH (1997) Parasites of fish and risks to public health. Rev Sci Tech Off Int Epizoot 16:652–660
Couture C, Measures L, Gagnon J, Desbiens C (2003) Human intestinal anisakiosis due to consumption of raw salmon. Am J Surg Pathol 27:1167–1172
D’Amelio S, Mathiopoulos KD, Santos CP, Pugachev ON, Webb SC, Picanço M, Paggi L (2000) Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: Ascaridoidea) defined by polymerase chain reaction-based restriction fragment length polymorphism. Int J Parasitol 30:223–226
Dick TA, Dixon BR, Choudhury A (1991) Diphyllobothrium, Anisakis and other fish-borne parasitic zoonoses. Southeast Asian J Trop Med Public Health 22 (Suppl):150–152
Fagerholm HP (1988) Incubation in rats of a nematodal larva from cod to establish its specific identity: Contracaecum osculatum, (Rudolphi). Parasitol Res 75:57–63
Farjallah S, Slimane BB, Blel H, Amor N, Said K (2006) Anisakid parasites of two forkbeards (Phycis blennoides and Phycis phycis) from the eastern Mediterranean coasts in Tunisia. Parasitol Res 100:11–17
Li AX, D’Amelio S, Paggi L, He F, Gasser RB, Lun ZR, Abollo E, Turchetto M, Zhu XQ (2005) Genetic evidence for the existence of sibling species within Contracaecum rudolphii (Hartwich, 1964) and the validity of Contracaecum septentrionale (Kreis, 1955) (Nematoda: Anisakidae). Parasitol Res 96:361–366
Li MW, Zhu XQ, Gasser RB, Lin RQ, Sani RA, Lun ZR, Jacobs DE (2006) The occurrence of Toxocara malaysiensis in cats in China, confirmed by sequence-based analyses of ribosomal DNA. Parasitol Res 99:554–557
Mattiucci S, Nascetti G, Cianchi R, Paggi L, Arduino P, Margolis L, Brattey J, Webb S, D’Amelio S, Orecchia P, Bullini L (1997) Genetic and ecological data on the Anisakis simplex complex, with evidence for a new species (Nematoda, Ascaridoidea, Anisakidae). J Parasitol 83:401–416
McCarthy J, Moore TA (2000) Emerging helminth zoonoses. Int J Parasitol 30:1351–1360
Nascetti G, Paggi L, Orecchia P, Smith JW, Mattiucci S, Bullini L (1986) Electrophoretic studies on the Anisakis simplex complex (Ascaridida: Anisakidae) from the Mediterranean and north-east Atlantic. Int J Parasitol 16:633–640
Nascetti G, Cianchi R, Mattiucci S, D’Amelio S, Orecchia P, Paggi L, Brattey J, Berland B, Smith JW, Bullini L (1993) Three sibling species within Contracaecum osculatum (Nematoda, Ascaridida, Ascaridoidea) from the Atlantic Arctic-Boreal region: reproductive isolation and host preferences. Int J Parasitol 23:105–120
Olson AC, Lewis MD, Hauser ML (1983) Proper identification of Anisakine worms. Am J Med Technol 49:111–114
Orecchia P, Mattiucci S, D’Amelio S, Paggi L, Plötz J, Cianchi R, Nascetti G, Arduino P, Bullini, L (1994) Two new members in the Contracaecum osculatum complex (Nematoda, Ascaridoidea) from the Antarctic. Int J Parasitol 24:367–377
Pellegrini M, Occhini R, Tordini G, Vindigni C, Russo S, Marzocca G (2005) Acute abdomen due to small bowel anisakiasis. Dig Liver Dis 37:65–67
Shih HH (2004) Parasitic helminth fauna of the cutlass fish, Trichiurus lepturus L., and the differentiation of four anisakid nematode third-stage larvae by nuclear ribosomal DNA sequences. Parasitol Res 93:188–195
Tang LQ, Zhang XL, Guo XA, Zhang MS, Liao YM (2001) Investigation of larval anisakids in sea fish sold in Shenzhen market. Chin J Zoon 17(1):103–104 (in Chinese)
Zhu XQ, Gasser RB, Podolska M, Chilton NB (1998) Characterisation of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences. Int J Parasitol 28:1911–1921
Zhu XQ, D’Amelio S, Palm HW, Paggi L, George-Nascimento M, Gasser RB (2002) SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda: Ascaridoidea: Anisakidae) using genetic markers in the internal transcribed spacers of ribosomal DNA. Parasitology 124:615–623