Assessing genetic diversity in a sugarcane germplasm collection using an automated AFLP analysis

Springer Science and Business Media LLC - 1998
P. Besse1, G. Taylor2, B. Carroll3, N. Berding4, D. Burner5, C.L. McIntyre1
1Commonwealth Scientific and Industrial Research Organisation (CSIRO), Tropical Agriculture, St Lucia, Qld, Australia
2Bureau of Sugar Experiment Stations (BSES), Indooroopilly, Qld, Australia
3Departments of Agriculture and Biochemistry, University of Queensland, Brisbane, Qld, Australia
4BSES, Meringa Experimental Station, Gordonvale, Qld, Australia
5USDA/ARS Sugarcane Research Unit, Houma, USA

Tóm tắt

An assessment of genetic diversity within and between Saccharum, Old World Erianthus sect. Ripidium, and North American E.giganteus (S.giganteum) was conducted using Amplified Fragment Length Polymorphism (AFLPTM) markers. An automated gel scoring system (GelComparTM) was successfully used to analyse the complex AFLP patterns obtained in sugarcane and its relatives. Similarity coefficient calculations and clustering revealed a genetic structure for Saccharum and Erianthus sect. Ripidium that was identical to the one previously obtained using other molecular marker types, showing the appropriateness of AFLP markers and the associated automated analysis in assessing genetic diversity in sugarcane. A genetic structure that correlated with cytotype (2n=30, 60, 90) was revealed within the North American species, E. giganteus (S.giganteum). Complex relationships among Saccharum, Erianthus sect. Ripidium, and North American E.giganteus were revealed and are discussed in the light of a similar study which involved RAPD markers.

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