Is the application of arbuscular mycorrhizal fungi an alternative to increase foliar phenolic compounds in seedlings of Mimosa tenuiflora (Wild.) Poir., Mimosoideae?
Tóm tắt
The legume Mimosa tenuiflora (Wild) Poir occurs in the “Caatinga” and is used as a popular remedy because of its medicinal properties related to the presence of secondary compounds in several parts of the plant. The arbuscular mycorrhizal fungi (AMF) are associated with most legumes, including M. tenuiflora, benefiting the growing process and the production of secondary compounds; however, the production of phytochemicals in response to AMF inoculation has not been defined yet. The aim of this study was to evaluate the influence of AMF on the production of phenolic compounds in the aerial part of M. tenuiflora. We analyzed the following parameters: height, stem diameter, number of leaves, fresh matter from the aerial part, dry matter from the aerial part, mycorrhizal colonization, soluble carbohydrates, total proteins, total phenols, total flavonoids, and total tannins. The mycorrhizal association did not benefit the growing process and/or the production of secondary compounds, demonstrating that mycorrhizal technology is not always an alternative to increase the production of plant secondary metabolism molecules. This study is the first report on the results of the inefficiency of mycorrhizal symbiosis regarding the optimization of phenolic compounds in plants of the “Caatinga”.
Tài liệu tham khảo
Albuquerque UP, Medeiros PM, Almeida ALS, Monteiro JM, Lins Neto EMF, Melo JG, Santos JP (2007) Medicinal plants of the caatinga (semi-arid) vegetation of NE Brazil: a quantitative approach. J Ethnopharmacol 114:325–354
Araújo TAS, Alencar NL, Amorim ELC, Albuquerque UP (2008) A new approach to study medicinal plants with tannins and flavonoids contents from the local knowledge. J Ethnopharmacol 120:72–80
Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Brito HO, Noronha EP, França LM, Brito LMO, Prado AS (2008) Análise da composição fitoquímica do extrato etanólico das folhas de Annona squamosa (ATA). Rev Bras Farm 89:180–184
Ceccarelli N, Curadi M, Martelloni L, Sbrana C, Picciarelli P, Giovannetti M (2010) Mycorrhizal colonization impacts on phenolic content and antioxidant properties of artichoke leaves and flower heads 2 years after field transplant. Plant Soil 335:311–323
Dubois M, Guiles A, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
Gattai GS, Pereira SV, Costa CMC, Lima CEP, Maia LC (2011) Microbial activity, arbuscular mycorrhizal fungi and inoculation of woody plants in lead contaminated soil. Braz J Microbiol 42:859–867
Geneva MP, Stancheva IV, Boychinova MM, Mincheva NH, Yonova PA (2010) Effects of foliar fertilization and arbuscular mycorrhizal colonization on Salvia officinalis L. growth, antioxidant capacity, and essential oil composition. J Sci Food Agric 90:696–702
Giovannetti M, Mosse B (1980) An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytol 84:489–500
Kapoor R, Giri B, Mukerji KG (2002) Glomus macrocarpum: a potential bioinoculant to improve essential oil quality and concentration in Dill (Anethum graveolens L.) and Carum (Trachyspermum ammi (linn.) Sprague). World J Microb Biot 18:459–463
Kapoor R, Giri B, Mukerji KG (2004) Improved growth and essential oil yield and quality in Foeniculum vulgare mill on mycorrhizal inoculation supplemented with P-fertilizer. Bioresour Technol 93:307–311
Lima CS, Campos MAS, Silva FSB (2015) Mycorrhizal fungi (AMF) increase the content of biomolecules in leaves of Inga vera Willd. seedlings. Symbiosis 65:117–123
Maia GN (2004) Caatinga: árvores e arbustos e suas utilidades, 1ª edn. D & Z computações Gráficas e Editora, São Paulo
Mandal S, Upadhyay S, Singh VP, Kapoor R (2015) Enhanced production of steviol glycosides in mycorrhizal plants: a concerted effect of arbuscular mycorrhizal symbiosis on transcription of biosynthetic genes. Plant Physiol Biochem 89:100–106
Mechri B, Tekaya M, Cheheb H, Attia F, Hammami M (2015) Accumulation of flavonoids and phenolic compounds in olive tree roots in response to mycorrhizal colonization: a possible mechanism for regulation of defense molecules. J Plant Physiol 185:40–43
Monteiro JM, Albuquerque UP, Lins Neto EMF, Araújo EL, Albuquerque MM, Amorim ELC (2006) The effects of seasonal climate changes in the Caatinga on tannin levels in Myracrodruon urundeuva (Engl.) Fr. All. and Anadenanthera colubrine (Vell.) Brenan. Braz J Pharmacog 16:338–344
Moreira FMS, Siqueira JO (2002) Micorrizas. In: Microbiologia e bioquímica do solo. Editora UFLA, Lavras
Oliveira PTF, Alves GD, Silva FA, Silva FSB (2015) Foliar bioactive compounds in Amburana cearensis (Allemao) A.C. Smith seedlings: increase of biosynthesis using mycorrhizal technology. J Med Plants Res 9:712–718
Pedone-Bonfim MVL, Lins MA, Coelho IR, Santana AS, Silva FSB, Maia LC (2013) Mycorrhizal technology and phosphorus in the production of primary and secondary metabolites in cebil (Anadenanthera colubrine (Vell.) Brenan) seedlings. J Sci Food Agric 93:1479–1484
Phillips JM, Hayman D (1970) Improved procedures for clearing roots and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infection. Trans Br Mycol Soc 55:158–161
Rosa-Mera CJ, Ferrera-Cerrato R, Alarcón A, Sánchez-Colin MJ, Muñoz-Muñiz OD (2011) Arbuscular mycorrhizal fungi and potassium bicarbonate enhance the foliar content of the vinblastine alkaloid in Catharanthus roseus. Plant Soil 349:367–376
Sanmartín C, Garmendia I, Romano B, Díaz M, Palop JA, Goicoechea N (2014) Mycorrhizal inoculation affected growth, mineral composition proteins and sugars in lettuces biofortified with organic or inorganic selene compounds. Sci Hort 180:40–51
Schüβler A, Schwarzott D, Walker C (2001) A new fungal phylum, the Glomeromycota: phylogeny and evolution. Micol Res 105:1413–1421
Silva FSB (2006) Fase assimbiótica, produção, infectividade e efetividade de fungos micorrízicos arbusculares (FMA) em substratos orgânicos. Tese de Doutorado, Recife
Silva FA, Silva FSB, Maia LC (2014a) Biotechnical application of arbuscular mycorrhizal fungi used in the production of foliar biomolecules in ironwood seedlings [Libidibia ferrea (Mart. ex Tul.) L.P.Queiroz var. ferrea]. J Med Plants Res 8:814–819
Silva FA, Ferreira MRA, Soares LAL, Sampaio EVSB, Silva FSB, Maia LC (2014b) Arbuscular mycorrhizal fungi increase gallic acid production in leaves of field grown Libidibia ferrea (Mart. ex. Tul.) LP Queiroz. J Med Plants Res 8:1110–1115
Smith SE, Read DJ (2008) Mycorrhizal symbiosis. Academic Press-Elsevier, New York
Souza RSO, Albuquerque UP, Monteiro JM, Amorim ELC (2008) Jurema-Preta (Mimosa tenuiflora [Willd.] Poir.): a review of its traditional use, phytochemistry and pharmacology. Braz Arch Biol Technol 51:937–947
Taiz L, Zeiger E (2013) Fisiologia vegetal, 5th edn. ArtMed, Porto Alegre
Toussaint JP (2007) Investigating physiological changes in the aerial parts of AM plants: what do we know and where should we be heading? Mycorrhiza 17:349–353
Urcoviche RC, Gazim ZC, Dragunski DC, Barcellos FG, Alberton O (2015) Plant growth and essential oil content of Mentha crispa inoculated with arbuscular mycorrhizal fungi under different levels of phosphorus. Ind Crop Prod 67:103–107
Zimare SB, Borde MY, Jite PK, Malpathak NP (2013) Effect of AM Fungi (Gf, Gm) on biomass and gymnemic acid content of Gymnema sylvestre (Retz.) R. Br. ex Sm. Proc Natl Acad Sci 83:439–445