Cdc42 induces filopodia by promoting the formation of an IRSp53:Mena complex

Hall, 1998, Rho GTPases and the actin cytoskeleton, Science, 279, 509, 10.1126/science.279.5350.509 Ridley, 1992, The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors, Cell, 70, 389, 10.1016/0092-8674(92)90163-7 Ridley, 1992, The small GTP-binding protein rac regulates growth factor-induced membrane ruffling, Cell, 70, 401, 10.1016/0092-8674(92)90164-8 Nobes, 1995, Rho, Rac and Cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia, Cell, 81, 53, 10.1016/0092-8674(95)90370-4 Kozma, 1995, The Ras-related protein Cdc42Hs and bradykinin promote formation of peripheral actin microspikes and filopodia in Swiss 3T3 fibroblasts, Mol Cell Biol, 15, 1942, 10.1128/MCB.15.4.1942 Bishop, 2000, Rho GTPases and their effector proteins, Biochem J, 348, 241, 10.1042/bj3480241 Burbelo, 1995, A conserved binding motif defines numerous candidate target proteins for both Cdc42 and Rac GTPases, J Biol Chem, 270, 29071, 10.1074/jbc.270.49.29071 Symons, 1996, Wiskott-Aldrich syndrome protein, a novel effector for the GTPase CDC42Hs, is implicated in actin polymerization, Cell, 84, 723, 10.1016/S0092-8674(00)81050-8 Kim, 2000, Autoinhibition and activation mechanism of the Wiscott-Aldrich syndrome protein, Nature, 404, 151, 10.1038/35004513 Lei, 2000, Structure of Pak1 in an autoinhibited conformation reveals a multistep activation switch, Cell, 102, 387, 10.1016/S0092-8674(00)00043-X Hoffman, 2000, Flipping the switch, Cell, 102, 403, 10.1016/S0092-8674(00)00045-3 Machesky, 1998, Scar1 and the related Wiscott-Aldrich syndrome protein, WASp, regulate the actin cytoskeleton through the Arp2/3 complex, Curr Biol, 8, 1347, 10.1016/S0960-9822(98)00015-3 Rohatgi, 2000, Mechanism of N-WASP activation by CDC42 and phosphatidylinositol 4, 5-bisphosphate, J Cell Biol, 150, 117, 10.1083/jcb.150.6.1299 Miki, 1998, Induction of filopodium formation by a WASP-related actin depolymerizing protein N-WASP, Nature, 391, 93, 10.1038/34208 Castellano, 1999, Inducible recruitment of Cdc42 or WASp to a cell surface receptor triggers actin polymerization and filopodium formation, Curr Biol, 9, 351, 10.1016/S0960-9822(99)80161-4 Mullins, 1998, The interaction of Arp2/3 complex with actin, Proc Natl Acad Sci USA, 95, 6181, 10.1073/pnas.95.11.6181 Svitkina, 1999, Arp2/3 complex and actin depolymerizing factor/cofilin in dendritic organization and treadmilling of actin filament array in lamellipodia, J Cell Biol, 145, 1009, 10.1083/jcb.145.5.1009 Martinez-Quiles, 2001, WIP regulates N-WASP-mediated actin polymerization and filopodium formation, Nat Cell Biol, 3, 484, 10.1038/35074551 Yeh, 1996, Characterization and cloning of a 58/53-kDa substrate of the insulin receptor tyrosine kinase, J Biol Chem, 271, 2921, 10.1074/jbc.271.6.2921 Miki, 2000, IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling, Nature, 408, 732, 10.1038/35047107 Govind, 2001, Cdc42Hs facilitates cytoskeletal reorganization and neurite outgrowth by localizing the 58-kD insulin receptor substrate to filamentous actin, J Cell Biol, 152, 579, 10.1083/jcb.152.3.579 Oda, 1999, Identification of BAIAP2 (BAI-associated protein2), a novel human homologue of hamster IRSp53, whose SH3 domain interacts with the cytoplasmic domain of BAI1, Cytogenet Cell Genet, 84, 75, 10.1159/000015219 Nagata, 1998, Activation of G1 progression, JNK MAP kinase and actin filament assembly by the exchange factor FGD1, J Biol Chem, 273, 15453, 10.1074/jbc.273.25.15453 Niebuhr, 1997, A novel proline-rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module in the Ena/VASP family, EMBO J, 16, 5433, 10.1093/emboj/16.17.5433 Gertler, 1996, Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics, Cell, 87, 227, 10.1016/S0092-8674(00)81341-0 Lanier, 1999, Mena is required for neurolation and commissure formation, Neuron, 22, 313, 10.1016/S0896-6273(00)81092-2 Goldberg, 2000, Recruitment of the Arp2/3 complex and Mena for the stimulation of actin polymerization in growth cones by nerve growth factor, J Neurosci Res, 60, 458, 10.1002/(SICI)1097-4547(20000515)60:4<458::AID-JNR4>3.0.CO;2-Z Bachmann, 1999, The EVH2 domain of the vasodilator-stimulated phosphoprotein mediates tetramerization, F-actin binding and actin bundle formation, J Biol Chem, 274, 23549, 10.1074/jbc.274.33.23549 Ermekova, 1997, The WW domain of neural protein FE65 interacts with proline-rich motifs in Mena, the mammalian homolog of Drosophila Enabled, J Biol Chem, 272, 32869, 10.1074/jbc.272.52.32869 Chakraborty, 1995, A focal adhesion factor directly linking intracellularly motile Listeria monocytogenes and Listeria ivanovii to the actin-based cytoskeleton of mammalian cells, EMBO J, 14, 1314, 10.1002/j.1460-2075.1995.tb07117.x Laurent, 1999, Role of proteins of the Ena/VASP family in actin-based motility of Listeria monocytogenes, J Cell Biol, 144, 1245, 10.1083/jcb.144.6.1245 Bear, 2000, Negative regulation of fibroblast motility by Ena/VASP proteins, Cell, 101, 717, 10.1016/S0092-8674(00)80884-3 Vasioukhin, 2000, Directed actin polymerization is the driving force for epithelial cell-cell adhesion, Cell, 100, 209, 10.1016/S0092-8674(00)81559-7 Gertler, 1990, Genetic suppression of mutations in the Drosophila abl proto-oncogene homolog, Science, 248, 857, 10.1126/science.2188361 Jay, 2000, The clutch hypothesis revisited, J Neurobiol, 44, 114, 10.1002/1097-4695(200008)44:2<114::AID-NEU3>3.0.CO;2-8 Abbott, 1999, The insulin receptor tyrosine kinase substrate p58/53 and the insulin receptor are components of CNS synapses, J Neurosci, 19, 7300, 10.1523/JNEUROSCI.19-17-07300.1999 Lambrechts, 2000, PKA phosphorylation of EVL, a Mena/VASP relative, regulates its interaction with actin and SH3-domains, J Biol Chem, 275, 36143, 10.1074/jbc.M006274200 Comer, 1998, Phosphorylation of Enabled by the Drosophila Abelson tyrosine kinase regulates the in vivo function and protein-protein interactions of Enabled, Mol Cell Biol, 18, 152, 10.1128/MCB.18.1.152 Aspenström, 1995, Yeast two hybrid system to detect protein-protein interactions with rho GTPases, Methods Enzymol, 256, 228, 10.1016/0076-6879(95)56027-0 Self, 1995, Measurements of intrinsic nucleotide exchange and GTP hydrolysis rates, Methods Enzymol, 256, 67, 10.1016/0076-6879(95)56010-6 Hartinger, 1996, 16-BAC/sds-Page, Anal Biochem, 240, 126, 10.1006/abio.1996.0339 Gevaert, 1997, Peptides adsorbed on reverse-phase chromatographic beads as targets for femtomole sequencing by post-source decay matrix assisted laser desorption ionization-reflectron time of flight mass spectrometry (MALDI-RETOF-MS), Electrophoresis, 18, 2950, 10.1002/elps.1150181537 Gevaert, 1998, A peptide concentration and purification method for protein characterization in the subpicomole range using matrix assisted laser desorption/ionization-postsource decay (MALDI-PSD) sequencing, Electrophoresis, 19, 909, 10.1002/elps.1150190606