Inhibition of Fura-2 sequestration and secretion with organic anion transport blockers

Cell Calcium - Tập 11 - Trang 57-62 - 1990
F Di Virgilio1,2, T.H Steinberg3, S.C Silverstein4
1C.N.R. Center for the Study of the Physiology of Mitochondria, University of Padova, Padova, Italy
2Institute of General Pathology, University of Padova, Padova, Italy
3Department of Medicine, Columbia University College of Physicians and Surgeons, New York, USA
4Rover Physiology Laboratories, Department of Physiology and Cellular Biophysics, Columbia University College of Physicians and Surgeons, New York, USA

Tài liệu tham khảo

Tsien, 1982, Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator, J. Cell Biol., 94, 325, 10.1083/jcb.94.2.325 Arslan, 1985, Cytosolic Ca2+ homeostasis in Ehrlich and Yoshida carcinomas, J. Biol. Chem., 260, 2719, 10.1016/S0021-9258(18)89421-2 Malgaroli, 1987, Fura-2 measurement of cytosolic free Ca2+ in monolayers and suspensions of various types of animal cells, J. Cell Biol., 105, 2145, 10.1083/jcb.105.5.2145 Steinberg, 1987, Fura-2 fluorescence is localized to mitochondria in endothelial cells, Am. J. Physiol., 253, C744, 10.1152/ajpcell.1987.253.5.C744 Almers, 1985, The Ca signal from Fura-2 loaded mast cells depends strongly on the method of dye-loading, FEBS Lett., 192, 13, 10.1016/0014-5793(85)80033-8 Di Virgilio, 1988, Fura-2 secretion and sequestration in macrophages. A blocker of organic anion transport reveals that these processes occur via a membrane transport system for organic anions, J. Immunol., 140, 915, 10.4049/jimmunol.140.3.915 Steinberg, 1987, Macrophages possess probenecid-inhibitable organic anion transporters that remove fluorescent dyes from the cytoplasmic matrix, J. Cell Biol., 105, 2695, 10.1083/jcb.105.6.2695 Steinberg, 1988, A prelysosomal compartment sequesters membrane impermeable fluorescent dyes from the cytoplasmic matrix of J774 macrophages, J. Cell Biol., 107, 887, 10.1083/jcb.107.3.887 Treves, 1987, Calcium and inositolphosphates in the activation of T cell-mediated cytotoxicity, J. Exp. Med., 166, 33, 10.1084/jem.166.1.33 Di Virgilio, 1988, Inhibitors of membrane transport system for organic anions block Fura-2 excretion from PC12 and N2A cells, Biochem. J., 256, 959, 10.1042/bj2560959 Fasolato, 1988, Generation of inositol phosphates, cytosolic Ca2+, and ionic fluxes in PC12 cells treated with bradykinin, J. Biol. Chem., 263, 17350, 10.1016/S0021-9258(19)77842-9 Steinberg, 1987, ATP4− permeabilizes the plasma membrane of mouse macrophages to fluorescent dyes, J. Biol. Chem., 262, 8884, 10.1016/S0021-9258(18)47497-2 Williams, 1985, Calcium gradients in single smooth muscle cells revealed by the digital imaging microscope using Fura-2, Nature, 318, 558, 10.1038/318558a0 Poenie, 1986, Calcium rises abruptly and briefly throughout the cell at the onset of anaphase, Science, 233, 886, 10.1126/science.3755550 Di Virgilio, 1988, Fc receptor-mediated phagocytosis occurs in macrophages at exceedingly low cytosolic Ca2+ levels, J. Cell Biol., 106, 657, 10.1083/jcb.106.3.657 Giligorski, 1986, Alpha1-adrenergic stimulation and cytoplasmic free calcium concentration in cultured renal proximal tubular cells: evidence for compartmentalization of quin-2 and Fura-2, J. Cell. Physiol., 128, 466, 10.1002/jcp.1041280316