Retinol and α-Tocopherol Content in the Liver and Skeletal Muscle of Bats (Chiroptera) during Hibernation and Summer Activity
Tóm tắt
In this work, the content of retinol and α-tocopherol in bats
living and wintering in Karelia at the northern periphery of their
natural habitats, was studied for the first time at different stages
of hibernation and during summer activity. A characteristic feature
of chiropterans of the northern area is staying most of the year
in a state of hypobiosis. Torpor–arousal cycles during hibernation are
associated with a rapid increase in body temperature and respiration,
which leads to an increase in the production of reactive oxygen
species. To study the antioxidant status in five bat species (Myotis brandtii, Myotis Mystacinus, Myotis Daubentonii, Plecotus auritus and Eptesicus nilssonii), retinol and
α-tocopherol levels were determined by HPLC in their liver and skeletal muscle.
It was found that the antioxidant content was higher in torpid bats
during hibernation compared to active animals in the summer period.
At the beginning of hibernation, a highest α-tocopherol level in
the liver was found in Eptesicus nilssonii,
while the retinol level was the highest in Plecotus
auritus. Retinol and α-tocopherol levels in the liver
and skeletal muscle of Myotis brandtii in
spring were higher compared to other species. At different stages
of hibernation, α-tocopherol levels in the skeletal muscle can be
higher than in the liver. In spring, before exiting hibernation,
females demonstrated a higher antioxidant level in tissues compared
to males. In all bat species, there was a significant variability
in indices, which can be explained by both species-specific and
individual differences among animals living in natural conditions.
High tissue tocopherol and retinol levels may play an important
role in the strategy of antioxidant defense in bats of the northern
area during hibernation.
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