Uncovering mechanosensing mechanisms at the single protein level using magnetic tweezers

Vogel, 2006, Local force and geometry sensing regulate cell functions, Nat. Rev. Mol. Cell Biol., 7, 265, 10.1038/nrm1890 Iskratsch, 2014, Appreciating force and shape—the rise of mechanotransduction in cell biology, Nat. Rev. Mol. Cell Biol., 15, 825, 10.1038/nrm3903 Neuman, 2008, Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy, Nat. Methods, 5, 491, 10.1038/nmeth.1218 Grashoff, 2010, Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics, Nature, 466, 263, 10.1038/nature09198 Stabley, 2012, Visualizing mechanical tension across membrane receptors with a fluorescent sensor, Nat. Methods, 9, 64, 10.1038/nmeth.1747 Chen, 2015, Dynamics of equilibrium folding and unfolding transitions of titin immunoglobulin domain under constant forces, J. Am. Chem. Soc., 137, 3540, 10.1021/ja5119368 Strick, 1998, Behavior of supercoiled dna, Biophys. J., 74, 2016, 10.1016/S0006-3495(98)77908-1 Yan, 2004, Near-field-magnetic-tweezer manipulation of single dna molecules, Phys. Rev. E, 70, 011905, 10.1103/PhysRevE.70.011905 De Vlaminck, 2012, Recent advances in magnetic tweezers, Annu. Rev. Biophys., 41, 453, 10.1146/annurev-biophys-122311-100544 De Vlaminck, 2012, Magnetic forces and dna mechanics in multiplexed magnetic tweezers, PLoS One, 7, e41432, 10.1371/journal.pone.0041432 Chen, 2011, Improved high-force magnetic tweezers for stretching and refolding of proteins and short dna, Biophys. J., 100, 517, 10.1016/j.bpj.2010.12.3700 Le, 2013, Mechanosensing of dna bending in a single specific protein-dna complex, Sci. Rep., 3, 3508, 10.1038/srep03508 Le, 2014, Mechanical force antagonizes the inhibitory effects of recx on reca filament formation in mycobacterium tuberculosis, Nucl. Acids Res., 42, 11992, 10.1093/nar/gku899 Fu, 2013, Force and atp hydrolysis dependent regulation of reca nucleoprotein filament by single-stranded dna binding protein, Nucl. Acids Res., 41, 924, 10.1093/nar/gks1162 Liu, 2009, Mechanical characterization of protein l in the low-force regime by electromagnetic tweezers/evanescent nanometry, Biophys. J., 96, 3810, 10.1016/j.bpj.2009.01.043 Lee, 2010, Minute negative superhelicity is sufficient to induce the b-z transition in the presence of low tension, Proc. Natl. Acad. Sci. USA, 107, 4985, 10.1073/pnas.0911528107 Long, 2013, Mechanical unfolding of human telomere g-quadruplex dna probed by integrated fluorescence and magnetic tweezers spectroscopy, Biophys. J., 106, 64a, 10.1016/j.bpj.2013.11.430 Qu, 2013, Mechanism of dna organization by mycobacterium tuberculosis protein lsr2, Nucl. Acids Res., 41, 5263, 10.1093/nar/gkt249 Lin, 2012, Physical organization of dna by multiple non-specific dna-binding modes of integration host factor (ihf), PLoS One, 7, e49885, 10.1371/journal.pone.0049885 Graham, 2011, Concentration-dependent exchange accelerates turnover of proteins bound to double-stranded dna, Nucl. Acids Res., 39, 2249, 10.1093/nar/gkq1140 Fu, 2011, Transition dynamics and selection of the distinct s-dna and strand unpeeling modes of double helix overstretching, Nucl. Acids Res., 39, 3473, 10.1093/nar/gkq1278 Zhang, 2012, Two distinct overstretched dna structures revealed by single-molecule thermodynamics measurements, Proc. Natl. Acad. Sci. USA, 109, 8103, 10.1073/pnas.1109824109 Zhang, 2013, Revealing the competition between peeled ssdna, melting bubbles, and s-dna during dna overstretching by single-molecule calorimetry, Proc. Natl. Acad. Sci. USA, 110, 3865, 10.1073/pnas.1213740110 You, 2014, Dynamics and stability of polymorphic human telomeric g-quadruplex under tension, Nucl. Acids Res., 42, 8789, 10.1093/nar/gku581 You, 2015, Stability and kinetics of c-myc promoter g-quadruplexes studied by single-molecule manipulation, J. Am. Chem. Soc., 137, 2424, 10.1021/ja511680u Yao, 2015, Thermodynamics of force-dependent folding and unfolding of small protein and nucleic acid structures, Integr. Biol., 10.1039/C5IB00038F Bustamante, 1994, Entropic elasticity of lambda-phage dna, Science, 265, 1599, 10.1126/science.8079175 Marko, 1995, Stretching dna, Macromolecules, 28, 8759, 10.1021/ma00130a008 Rouzina, 2001, Force-induced melting of the dna double helix 1. Thermodynamic analysis, Biophys. J., 80, 882, 10.1016/S0006-3495(01)76067-5 Cocco, 2004, Overstretching and force-driven strand separation of double-helix dna, Phys. Rev. E, 70, 011910, 10.1103/PhysRevE.70.011910 Gao, 2012, Single reconstituted neuronal snare complexes zipper in three distinct stages, Science, 337, 1340, 10.1126/science.1224492 Yao, 2014, Force-dependent conformational switch of α-catenin controls vinculin binding, Nat. Commun., 5, 4525, 10.1038/ncomms5525 Yao, 2014, Mechanical activation of vinculin binding to talin locks talin in an unfolded conformation, Sci. Rep., 4, 4610, 10.1038/srep04610 Trinick, 1996, Cytoskeleton: titin as a scaffold and spring, Curr. Biol., 6, 258, 10.1016/S0960-9822(02)00472-4 Fernandez, 2004, Force-clamp spectroscopy monitors the folding trajectory of a single protein, Science, 303, 1674, 10.1126/science.1092497 Rognoni, 2012, Dynamic force sensing of filamin revealed in single-molecule experiments, Proc. Natl. Acad. Sci. USA, 109, 19679, 10.1073/pnas.1211274109 Chen, 2011, Differential mechanical stability of filamin a rod segments, Biophys. J., 101, 1231, 10.1016/j.bpj.2011.07.028 Chen, 2013, Mechanical perturbation of filamin a immunoglobulin repeats 20–21 reveals potential non-equilibrium mechanochemical partner binding function, Sci. Rep., 3, 1642, 10.1038/srep01642 del Rio, 2009, Stretching single talin rod molecules activates vinculin binding, Science, 323, 638, 10.1126/science.1162912 Yonemura, 2010, [alpha]-catenin as a tension transducer that induces adherens junction development, Nat. Cell Biol., 12, 533, 10.1038/ncb2055 Yan, 2015, Talin dependent mechanosensitivity of cell focal adhesions, Cell Mol. Bioeng., 8, 151, 10.1007/s12195-014-0364-5 Liang, 2015, Current perspectives on cadherin-cytoskeleton interactions and dynamics, Cell Health Cytoskeleton, 7, 11 Lebel, 2014, Gold rotor bead tracking for high-speed measurements of dna twist, torque and extension, Nat. Methods, 11, 456, 10.1038/nmeth.2854 Le, 2015, Disturbance-free rapid solution exchange for magnetic tweezers single-molecule studies, Nucl. Acids Res., 10.1093/nar/gkv554