Probiotic fermented milk consumption modulates the allergic process induced by ovoalbumin in mice

British Journal of Nutrition - Tập 114 Số 4 - Trang 566-576 - 2015
Eva María del Mar Vélez1, Carolina Maldonado Galdeano1,2, Estéban Carmuega3, R Weill4, María Eugenia Bibas Bonet2, Gabriela Perdigón1,2
1Centro de Referencia para Lactobacilos, CERELA-CONICET, Chacabuco 145, San Miguel de Tucumán-Tucumán, Argentina
2Cátedra de Inmunología, Instituto de Microbiología, Facultad de Bioquímica, Química y Farmacia, UNT, Argentina
3CESNI (Centro de Estudios Nutricionales Infantiles), Buenos Aires, Argentina
4Director Científico Latam, DANONE Argentina S.A., Buenos Aires, Argentina

Tóm tắt

Orally administered probiotic micro-organisms are able to regulate the exacerbated immune response during the antigenic sensitisation process. The aim of the present study was to evaluate the potential efficacy of probiotic fermented milk (PFM) in preventing or treating allergy in an experimental model, and to investigate its underlying mechanisms. Ovoalbumin (OVA)-sensitised BALB/c mice were fed with PFM before the sensitisation procedure or fed continuously with PFM. At 7 and 15 d post-sensitisation, anti-OVA-specific IgE, IgG, IgG1 and IgG2a concentrations were measured in the serum and broncho-alveolar lavage fluid (BALF). Concentrations of interferon-γ (IFN-γ), IL-4, IL-10 and total secretory IgA (S-IgA) were measured in the supernatants of macerated lungs or in the BALF. The levels of IgA+, CD4+and CD8+T lymphocytes and F4/80+cells were measured in the lungs by immunofluorescence. Inducible CD4+/CD25/Foxp3+regulatory T (Treg) cells were evaluated in the lungs. PFM shifted the T helper (Th)2 profile response towards a Th1 response that led to the production of IgG instead of IgE, with increasing levels of IL-10 and IFN-γ that play an important role in immunomodulation exerted by PFM administration in sensitised mice. Anti-OVA-specific IgE levels were significantly decreased; however, there was no modification in the levels of anti-OVA-specific IgG and total S-IgA. PFM did not influence Tregcells in treated mice. Consumption of PFM could be a promising strategy in the amelioration of airway allergies, considering that the effect is mediated by the production of IgG through the activation of Th1 instead of the direct activation of Th2 cells to produce IgE.

Từ khóa


Tài liệu tham khảo

10.1016/j.ijfoodmicro.2010.01.020

10.1017/S0022029909990021

Maldonado Galdeano, 2015, Stimulation of innate immune cells induced by probiotics: participation of Toll-like receptors, J Clin Cell Immunol, 6, 283

10.1164/rccm.200806-951OC

10.1016/S0140-6736(00)04259-8

10.2334/josnusd.53.147

Becker, 2006, Respiratory syncytial virus (RSV) evades the human adaptive immune system by skewing the Th1/Th2 cytokine balance toward increased levels of Th2 cytokines and IgE, markers of allergy – a review, Virus Genes, 33, 235, 10.1007/s11262-006-0064-x

10.1016/S0140-6736(03)13490-3

10.1038/nri2097

Guo, 2010, Cholesterol-lowering effects of probiotics – a review, Wei Sheng Wu Xue Bao, 50, 1590

10.1186/1472-6882-14-365

FAO/WHO (2001) Evaluation of health and nutritional properties of powder milk and live lactic acid bacteria. Food and Agriculture Organization of the United Nations and World Health Organization Expert Consultation Report. ftp://ftp.fao.org/docrep/fao/009/a0512e/a0512e00.pdf.

10.1271/bbb.67.951

10.1016/j.it.2008.07.002

10.1016/j.imbio.2007.07.002

10.1038/sj.ejcn.1601658

10.1111/j.1365-2222.2007.02671.x

10.1111/j.1365-2567.2004.01925.x

10.1111/j.1348-0421.2006.tb03842.x

10.1128/CVI.13.2.219-226.2006

10.4168/aair.2010.2.3.199

10.1111/j.1398-9995.2007.01462.x

10.1002/jsfa.4214

10.1248/bpb.28.1462

10.1016/j.amjsurg.2006.11.026

10.1038/sj.ejcn.1601658

10.1186/1471-2172-9-27

10.1159/000234841

10.1128/CVI.00406-06

10.1111/j.1365-2222.2006.02629.x

10.1016/j.pupt.2012.05.011

Wu, 2014, Smooth muscle progenitor cells involved in the development of airway remodeling in a murine model of asthma, Asian Pac J Allergy Immunol, 32, 203

10.1164/rccm.200606-821OC

10.1111/j.1574-695X.2012.00981.x

10.1111/all.12274

10.1186/1471-230X-11-64

10.4049/jimmunol.0900215

Ouwehand, 2007, Antiallergic effects of probiotics, J Nutr, 137, 794S, 10.1093/jn/137.3.794S

10.1016/j.smim.2013.09.003

10.1097/ACI.0b013e32832abfc2

Thông tin
Thông tin xuất bản

British Journal of Nutrition

Tập 114 Số 4

566-576

Thông tin tác giả