Hepatoprotective role of berberine against paraquat-induced liver toxicity in rat

Springer Science and Business Media LLC - Tập 27 - Trang 4969-4975 - 2019
Aziz Eftekhari1, Amir Hasanzadeh1, Rovshan Khalilov2,3,4, Hasan Hosainzadegan1, Elham Ahmadian5,6, Mohammad Ali Eghbal7
1Maragheh University of Medical Sciences, Maragheh, Iran
2Russian Institute for Advanced Study, Moscow State Pedagogical University, Moscow, Russian Federation
3Department of Biophysics and Molecular Biology, Baku State University, Baku, Azerbaijan
4Joint Ukraine-Azerbaijan International Research and Education Center of Nanobiotechnology and Functional Nanosystems, Drohobych, Ukraine & Baku, Azerbaijan
5Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
6Students Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
7Pharmacology and Toxicology Department, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

Tóm tắt

Paraquat (PQ) is a herbicide agent commonly used in agricultural applications. Hepatotoxicity is among clinical complications associated with PQ intoxication. Oxidative stress and its subsequent events are major mechanisms identified in PQ-induced liver toxicity. Berberine (BBR) is a natural antioxidant widely investigated for its hepatoprotective effects. The present study designed to evaluate the potential cytoprotective properties of BBR against PQ-induced cytotoxicity in primary cultured rat hepatocytes and in vivo test of liver function enzymes. Cellular and biochemical parameters including lactate dehydrogenase (LDH), cell viability, ROS formation, glutathione (GSH) content, and mitochondrial membrane potential in the PQ-treated hepatocytes were measured, and the mentioned markers were evaluated in the presence of BBR. BBR treatment caused significant decrease in PQ-induced cell death, ROS formation, and LDH release. On the other hand, it was found that BBR inhibits cellular glutathione depletion in PQ-treated hepatocytes. Also, BBR treatment significantly diminished PQ-induced the liver function enzyme elevation. These data mention the potential hepatoprotective effect of BBR with therapeutic capability against PQ-induced liver damage.

Tài liệu tham khảo

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