Cadmium Accumulation and Antioxidative Responses in the Sesbania drummondii Callus

Springer Science and Business Media LLC - Tập 50 - Trang 121-127 - 2005
M Israr1, S V Sahi1, J Jain2
1Department of Biology, Western Kentucky University, Bowling Green, USA
2Department of Civil Engineering and Geological Sciences, University of Notre Dame, Notre Dame, Indiana, USA

Tóm tắt

The effect of cadmium (Cd) on growth, accumulation, and antioxidative response was studied in Sesbania drummondii callus, cultivated on different concentrations of Cd (0–250 μM) for four weeks. Callus growth was comparable to that of the control for concentrations up to 50 μM Cd; however, concentrations higher than 50 μM affected growth. A concentration of 100 μM Cd inhibited growth by 16%, with respect to control. Cd concentration in callus increased with increasing Cd concentrations in the growth medium. Callus accumulated 530 mg Cd kg−1 of their dry weight at 100 μM Cd concentration. Sesbania callus responded to Cd-induced oxidative stress by modulating antioxidants (glutathione and other non-protein thiols) level and antioxidative enzymes: superoxide dismutase (SOD), ascorbate peroxidase (APX), and glutathione reductase (GR). The content of the glutathione (GSH) and GSH/GSSG ratio first increased up to a concentration of 50 μM Cd and then decreased. The content of other non-protein thiols significantly increased with increasing Cd concentrations in the growth medium. The activities of antioxidative enzymes, SOD, APX, and GR, followed the same trends as antioxidants first increasing up to a concentration of 50 μM Cd and then decreasing. These results suggest that antioxidative defense mechanisms play a significant role in Cd detoxification and accumulation in Sesbania drummondii.

Tài liệu tham khảo

Anderson ME (1985) Determination of glutathione and glutathione disulfide in biological samples. Method Enzymol 113:548–554 Asada K, Takahashi M (1987) Production and scavenging of active oxygen in photosynthesis. In: Kyle DJ, Osmond CJ, Arntzen CJ (ed) Photo inhibition: Topics in Photosynthesis, Elsevier, Amsterdam, p 227 Barcelo J, Poschenrieder C (1990) Plant water relation as affected by heavy metal stress: review. J Plant Nutr 13:1–37 Bartosz G (1996) Glutathione as antioxidant and electrophile scavenger. Pol J Environ Stud 5:87–88 Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254 Broadbent P, Creissen GP, Kular B, Wellburn AR, Mullineaux PM (1995) Oxidative stress responses in transgenic tobacco containing altered levels of glutathione reductase activity. Plant J 8:247–255 Brooks RR (1998) Plants that hyperaccumulate heavy metals. CAB International, Wallingford, UK Bueno P, Piqueras A (2002) Effect of transition metals on stress, lipid peroxidation and antioxidant enzyme activities in tobacco cell cultures. Plant Growth Regul 36:161–167 Cao X, Ma LQ, Tu C (2004) Antioxidative responses to arsenic in the arsenic hyperaccumulator Chinese brake fern (Pteris vittata L.). Environ Pollut 128:317–325 Chaoui A, Mazhouri S, Ghorbal MH, Ferjani EE (1997) Cadmium and zinc induction of lipid peroxidation and effects on antioxidant enzyme activities in bean (Phaseolus vulgaris L.). Plant Sci 127:139–147 Cheepala SB, Sharma NC, Sahi SV (2004) Rapid in vitro regeneration of Sesbania drummondii. Biol Plant 48:13–18 Cobbett CS (2000) Phytochelatins and their roles in heavy metal detoxification. Plant Physiol 123:825–832 Del longo OT, Gonzalez CA, Pastori GM, Trippi VS (1993) Antioxidant defenses under hyperoxygenic and hyperosmotic conditions in leaves of two lines of maize with differential sensitivity to drought. Plant Cell Physiol 34:1023–1028 Fodor A, Szabo-Nagy A, Erdei L (1995) The effect of cadmium on the fluidity and H+- ATPase activity of plasma membrane from sunflower and wheat roots. J Plant Physiol 14:787–792 Foyer CH, Lopez-Delgado H, Dat JF, Scott IM (1997) Hydrogen peroxide and glutathione associated mechanisms of acclamatory stress tolerance and signaling. Physiol Plant 100:241–254 Foyer CH, Souriau N, Perret S, Lelandais M, Kunert KJ, Pruvost C, Jouanin L (1995) Over-expression of glutathione reductase but not glutathione synthetase leads to increase in antioxidant capacity and improved photosynthesis in poplar (Populus tremula x P. alba ) tress. Plant Physiol 109:1047–1057 Freeman JL, Persans MW, Nieman K, Albrecht C, Peer W, Pickering I, Salt DE (2004) Increased glutathione biosynthesis plays a role in nickel tolerance in Thlaspi nickel hyperaccumulators. Plant Cell 16:2176–2191 Gallego SM, Benavides MP, Tomaro ML (1996) Effect of heavy metal ion excess on sunflower leaves: evidence for involvement of oxidative stress. Plant Sci 121:151–159 Gallego S, Benavides M, Tomaro M (2002) Involvement of an antioxidant defense system in the adaptive response to heavy metal ions in Helianthus annuus L. cells. Plant Growth Regul 36:267–273 Gregger M (1999) Metal availability and bioconcentration in plants. In: Hagemeyer J (ed) Heavy metals stress in plants: from molecules to ecosystems. Springer, Berlin, p 1 Horsch RB, King J, Jones GE (1980) Measurement of cultured plant cell growth on filter paper discs. Can J Bot 58:2402–2406 Jiang W, Liu D, Hou W (2001) Hyperaccumulation of cadmium by roots, bulbs and shoots of garlic (Allium sativum L.). Bioresource Technol 76:9–13 Kampfenkel K, Van Montagu M, Inze D (1995) Effects on iron excess on Nicotiana plumbaginifolia plants. Plant Physiol 107:725–735 Karataglis S, Moustakas M, Symeonidis L (1991) Effect of heavy metals on isoperoxidases of wheat. Biol Plant 33:3–9 Kato M, Shimizu S (1987) Chlorophyll metabolism in higher plants VII. Chlorophyll degradation in senscing tobacco leaves: phenolic dependant peroxidative degradation. Can J Bot 65:729–735 Koricheva J, Roy S, Vranjic JA, Haukioja E, Hughes PR, Hanninen O (1997) Antioxidants responses to stimulated acid rain and heavy metal deposition in birch seedlings. Environ Pollut 95:249–258 Krupa Z (1988) Cadmium induced changes in the composition and structure of the light harvesting complex II in radish cotyledons. Physiol Plant 73:518–524 Larsson EH, Bordman JF, Asp H (1998) Influence of UV-B radiation and Cd2+ on chlorophyll fluorescence, growth and nutrient content in Brassica napus. J Exp Bot 49:1031–1039 Lockwood MP (1976) Effects of pollutants on aquatic organisms. Cambridge University Press, New York Mazhoudi S, Chaoui A, Ghorbal MH, Ferjani EE (1997) Response of antioxidant enzymes to excess copper in tomato (Lycopersicon esculentum, Mill.). Plant Sci 127:129–137 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497 Nagalakshmi N, Prasad MNV (2001) Responses of glutathione cycle enzymes and glutathione metabolism to copper stress in Scenedesmus bijugatus. Plant Sci 160:291–299 Nakano Y, Asada K (1987) Purification of ascorbate peroxidase in spinach chloroplasts: its inactivation in ascorbate depleted medium and reactivation by monodehydroascorbate radical. Plant Cell Physiol 28:131–140 Nriagu JO, Pacyna JM (1988) Quantitative assessment of world wide contamination of air, water and soils with trace metals. Nature 333:134–139 Okamoto OK, Asano CS, Aidar E, Colepicolo P (1996) Effects of cadmium on growth and superoxide dismutase activity of the marine microalga Tetraselmis gracilis (Prasinophaceae). J Phycol 32:74–79 Patra J, Panda BB (1998) A comparison of biochemical responses to oxidative and metal stress in seedlings of barley, Hordeum vulgare L. Environ Pollut 101:99–105 Polle A, Rennenberg H (1994) Photooxidative stress in trees. In: Foyer CH, Mullineaux PM (ed) Causes of photooxidative stress and amelioration of defense system in plants. CRC Press, Boca Raton FL, p 199 Prasad KVSK, Saradhi PP, Sharmila P (1999) Concerted action of antioxidant enzymes and curtailed growth under zinc toxicity in Brassica juncea. Environ Exp Bot 42:1–10 Quariti O, Boussama N, Zarrouk M, Cherif A, Ghorbal MH (1997) Cadmium and copper induced changes in tomato membrane lipids. Phytochemistry 45:1343–1350 Rao MV, Paliyath G, Ormrod DP (1996) Ultraviolet- B and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. Plant Physiol 110:125–136 Rosen BP (2002) Biochemistry of arsenic detoxification. FEBS Lett 529:86–92 Rucinska R, Waplak S, Gwozoz EA (1999) Free radical formation and activity of antioxidant enzymes in lupin roots exposed to lead. Plant Physiol Biochem 37:187–194 Ruley AT (2004) Effects of accumulation of lead and synthetic chelators on the physiology and biochemistry of Sesbania drummondii. M.S. Thesis, Western Kentucky University, Bowling Green, KY. Sahi SV, Bryant NL, Sharma NC, Singh SR (2002) Characterization of a lead hyperaccumulator shrub, Sesbania drummondii. Environ Sci Technol 36:4676–4680 Schickler H, Caspi H (1999) Response of antioxidative enzymes to nickel and cadmium stress in hyperaccumulator plants of the genus Alyssum. Physiol Plant 105:39–44 Schneegurt MA, Jain JC, Menicuccu JA, Jr Brown S, Garafalo DF, Quallick M, Neal CR, Kulpa CF (2001) Biomass products for the remediation of wastewaters contaminated with toxic metals. Environ Sci Technol 35:3786–3791 Scott N, Hatlelid KM, MacKenzie NE, Carter DE (1993) Reactions of arsenic (III) and arsenic (V) species with glutathione. Chem Res Toxicol 6:102–106 Sidlecka A, Baszynsky T (1993) Inhibition of electron flow around photosystem I in chloroplast of cadmium treated maize plants is due to cadmium induced iron deficiency. Physiol Plant 87:199–202 Sobkowiak R, Rymer K, Rucinska R, Deckert J (2004) Cadmium-induced changes in antioxidant enzymes in suspension culture of soybean cells. Acta Biochim Polini 51:219–222 Srivastava S, Tripathi RD, Dwivedi UN (2004) Synthesis of phytochelatins and modulation of antioxidants in response to cadmium stress in Cuscuta reflexa-an angiospermic parasite. J Plant Physiol 161:665–674 Stobart AK, Griffiths WT, Ameen-Bukhari I, Sherwood RP (1985) The effect of Cd2+ on the biosynthesis of chlorophyll in leaves of barley. Physiol Plant 63:293–298 Stohs SJ, Bagchi D (1995) Oxidative mechanism in the toxicity of metal ions. Free Radical Biology and Medicine 18:321–336 Strid A (1993) Increased expression of defense genes in Pisum sativum after exposure to supplementary ultraviolet –B radiation. Plant Cell Physiol 34:949–953 Stroinski A, Kubis J, Zielezinska M (1999) Effect of cadmium on glutathione reductase in potato tubers. Acta Physiol Plant 21:201–207 Weckx J, Clijsters H (1996) Oxidative damage and defense mechanism in primary leaves of Phaseolus vulgaris as a result of root assimilation of toxic amounts of copper. Physiol Plant 96:506–512 Zhu YL, Pilon- Smits EAH, Jouanin L, Terry N (1999) Overexpression of glutathione synthetase in Indian mustard enhances cadmium accumulation and tolerance. Plant Physiol 119:73–80