Repeated cleaning of protein A affinity column with sodium hydroxide

Bio-Rad, 1990, Antibody purification for the preparative scale laboratory, 55 Bloom, 1989, Detection and reduction of protein A contamination in immobilized protein A purified monoclonal antibody preparations, J. Immunol. Methods, 117, 83, 10.1016/0022-1759(89)90121-X Brown, 1981, Laboratory evaluation of selected disinfectants as virucidal agents against porcine parvovirus, pseudorabies virus and transmissible gastroenteritis virus, Am. J. Vet. Res., 42, 1033 Clark, 1986, The detection and characterization of antigen-specific monoclonal antibodies using anti-immunoglobulin isotype antibodies coupled to red blood cells, Methods Enzymol., 121, 548, 10.1016/0076-6879(86)21054-X Coombs, 1987, Assays using red cell-labelled antibodies, J. Immunol. Methods, 101, 1, 10.1016/0022-1759(87)90209-2 Drulak, 1984, The effectiveness of six disinfectants in inactivation of reovirus-3, Microbios, 41, 31 Fuglistaller, 1989, Comparison of immunoglobulin binding capacities and ligand leakage using eight different protein A affinity chromatography matrices, J. Immunol. Methods, 124, 171, 10.1016/0022-1759(89)90350-5 Girot, 1990, Composite affinity sorbents and their cleaning in place, J. Chromatogr., 510, 213, 10.1016/S0021-9673(01)93755-0 Godfrey, 1992, A sensitive enzyme-linked immunosorbent assay (ELISA) for the detection of staphylococcal protein A (SpA) present as a trace contaminant of murine immunoglobulins purified on immobilized protein A, J. Immunol. Methods, 149, 21, 10.1016/S0022-1759(12)80044-5 Goding, 1978, Use of staphylococcal protein A as an immunological reagent, J. Immunol. Methods, 20, 241, 10.1016/0022-1759(78)90259-4 Hale, 1987, Isolation of low-frequency class-switch variants from rat hybrid myelomas, J. Immunol. Methods, 103, 59, 10.1016/0022-1759(87)90242-0 Johansson, 1987, Determination of the leakage from phenyl-Sepharose CL-4B, phenyl-Sepharose FF and phenyl-Superose in bulk and column experiments, J. Chromatogr., 403, 85, 10.1016/S0021-9673(00)96343-X Langone, 1982, Application of immobilized protein A in immunochemical techniques, J. Immunol. Methods, 55, 277, 10.1016/0022-1759(82)90088-6 Mitra, 1986, Elimination of infectious retroviruses during preparation of immunoglobulins, Transfusion, 26, 394, 10.1046/j.1537-2995.1986.26486262753.x Peng, 1986, Stability of antibody attachment in immunosorbent chromatography, Enzyme Microb. Technol., 8, 681, 10.1016/0141-0229(86)90066-9 Pharmacia LKB Biotechnology, 1989, Sanitization of BioPilot system and columns using sodium hydroxide Riechmann, 1988, Reshaping human antibodies for therapy, Nature, 332, 323, 10.1038/332323a0 Spire, 1984, Inactivation of lymphadenopathy associated virus by chemical disinfectants, Lancet, 2, 899, 10.1016/S0140-6736(84)90657-3 Talmadoe, 1989, Efficient endotoxin removal with a new sanitizable affinity column: Affi-Prep Polymyxin, J. Chromatogr., 476, 175, 10.1016/S0021-9673(01)93867-1 Taylor, 1991, Inactivation of BSE agent, Dev. Biol. Standard, 75, 97 Somlyo, 1992, Molecular requirements of endotoxin (ET) actions: changes in the immune adjuvant, TNF liberating and toxic properties of endotoxin during alkaline hydrolysis, Int. J. Immunopharmacol., 14, 131, 10.1016/0192-0561(92)90023-E