A gene regulatory network in mouse embryonic stem cells

Proceedings of the National Academy of Sciences of the United States of America - Tập 104 Số 42 - Trang 16438-16443 - 2007
Qing Zhou1, Hiram Chipperfield2, Douglas A. Melton2, Wing Hung Wong3
1Department of Statistics, University of California, Los Angeles, 8125 Math Science Building, Los Angeles, CA 90095, USA.
2Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138; and
3Departments of Statistics, Health Research and Policy, and Biological Sciences, Stanford University, 390 Serra Mall, Stanford, CA 94305

Tóm tắt

We analyze new and existing expression and transcription factor-binding data to characterize gene regulatory relations in mouse ES cells (ESC). In addition to confirming the key roles of Oct4, Sox2, and Nanog, our analysis identifies several genes, such as Esrrb, Stat3, Tcf7, Sall4, and LRH-1, as statistically significant coregulators. The regulatory interactions among 15 core regulators are used to construct a gene regulatory network in ESC. The network encapsulates extensive cross-regulations among the core regulators, highlights how they may control epigenetic processes, and reveals the surprising roles of nuclear receptors. Our analysis also provides information on the regulation of a large number of putative target genes of the network.

Từ khóa


Tài liệu tham khảo

10.1038/sj.onc.1207930

10.1101/gad.12.13.2048

10.1038/nature04915

10.1101/gad.1020502

10.1128/MCB.25.9.3492-3505.2005

10.1016/j.cell.2006.07.024

10.1038/ncb1211

10.1242/dev.02457

10.1016/S0092-8674(00)81769-9

10.1038/74199

10.1242/dev.122.3.881

10.1038/ng1760

10.1038/nature04733

10.1038/ncb1481

10.1016/j.mod.2004.01.006

Q Wu, X Chen, J Zhang, YH Loh, TK Low, W Zhang, W Zhang, SK Sze, B Lim, HH Ng J Biochem 281, 24090–24094 (2006).

10.1101/gr.3715005

10.1038/nm979

10.1016/j.molcel.2004.07.009

10.1126/science.1076469

10.1128/MCB.25.23.10379-10390.2005

10.1242/dev.129.11.2733

R Jager, U Werling, S Rimpf, A Jacob, H Schorle Mol Cancer Res 1, 921–929 (2003).

10.1128/MCB.19.8.5453

10.1128/MCB.25.14.6031-6046.2005

10.1038/nature05284

10.1242/dev.01107

10.1242/dev.122.1.243

10.1128/MCB.23.1.238-249.2003

10.1038/nature04433

Thông tin
Thông tin xuất bản

Proceedings of the National Academy of Sciences of the United States of America

Tập 104 Số 42

16438-16443

Thông tin tác giả