Binding of 21 Thiol Reagents to Human Hemoglobin in Solution and in Intact Cells

FEBS Journal - Tập 123 Số 3 - Trang 513-519 - 1982
Marie Claude Garel1, Yves Beuzard1, Joëlle Thillet1, Chantal Domenget1, Josiane MARTIN1, F. Galactéros1, Jean Rosa1
1Unité de Recherches sur les Anémies (Unite 91 de I'lnstitut National de la Santé et de la Recherche Médicale), Hôpital Henri‐Mondor,51 Avenue du Maréchal‐de‐Lattre‐de‐Tassigny, F‐94010 Créteil, Val‐de‐Marne, France

Tóm tắt

The reactivity of the cysteine‐β93 residue of human hemoglobin was investigated in order to define the optimal structure of potential antisickling agents. The properties of 21 thiol reagents were compared with regard to (a) their binding rate to hemoglobin in solution and within intact cells; (b) the modification of the oxygen dissociation curve of intact cells and (c) the effect on methemoglobin formation in solution or within intact cells. The results show the very different behaviors of these reagents.

Từ khóa


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Thông tin xuất bản

FEBS Journal

Tập 123 Số 3

513-519

Thông tin tác giả