A validated HPTLC method for the simultaneous quantifications of three phenolic acids and three withanolides from Withania somnifera plants and its herbal products

Bhat, 2015, A decade of molecular understanding of withanolide biosynthesis and in vitro studies in Withania somnifera (L.) Dunal: prospects and perspectives for pathway engineering, Front. Plant Sci., 6, 1031 Gupta, 2007, A validated and densitometric HPTLC method for the quantification of withaferin A and withanolide A in different plant parts of two morphotypes of Withania somnifera, Chromatogra, 66, 801, 10.1365/s10337-007-0396-2 Mirjalili, 2009, Steroidal lactones from Withania somnifera, an ancient plant for novel medicine, Molecules, 14, 2373, 10.3390/molecules14072373 Agarwal, 1999, Studies on immunomodulatory activity of Withania somnifera (Ashwagandha) extracts in experimental immune inflammation, J. Ethnopharmacol., 67, 27, 10.1016/S0378-8741(99)00065-3 Chandra, 2016, Quantitative determination of multi markers in five varieties of Withania somnifera using ultra-high performance liquid chromatography with hybrid triple quadrupole linear ion trap mass spectrometer combined with multivariate analysis: application to phar, J. Pharm. Biomed. Anal., 129, 419, 10.1016/j.jpba.2016.07.032 Bansal, 2004, Cardioprotection from ischemia and reperfusion injury by Withania somnifera: a hemodynamic, biochemical and histopathological assessment, Mol. Cell. Biochem., 260, 39, 10.1023/B:MCBI.0000026051.16803.03 Kulkarni, 2008, Effect of Withania somnifera dunal root extract against pentylenetetrazol seizure threshold in mice: possible involvement of GABAergic system, Indian J. Exp. Biol., 46, 465 Ziauddin, 1996, Studies on the immunomodulatory effects of Ashwagandha, J. Ethnopharmacol., 50, 69, 10.1016/0378-8741(95)01318-0 Elberry, 2010, Antihepatotoxic effect of marrubium vulgare and Withania somnifera extracts on carbon tetrachloride-induced hepatotoxicity in rats, J. Basic Clin. Pharm., 1, 247 Pawar, 2011, Rectal gel application of Withania somnifera root extract expounds anti-inflammatory and muco-restorative activity in TNBS-induced inflammatory bowel disease, BMC Complement. Altern. Med., 11, 34, 10.1186/1472-6882-11-34 Marcovic, 2015, Chemistry and pharmacology of Withania somnifera: an update, Tang Humanitas Med., 5, 1.1, 10.5667/tang.2014.0030 Roy, 2013, Withaferin a, a steroidal lactone from Withania somnifera, induces mitotic catastrophe and growth arrest in prostate cancer cells, J. Nat. Prod., 76, 1909, 10.1021/np400441f Mekbib, 2009, Evaluation of ethiopian plant extracts, acacia seyal and Withania somnifera, to control green mould and ensure quality maintenance of citrus (Citrus sinensis L.), Fruits, 64, 285, 10.1051/fruits/2009023 Khalil, 2011, High catechin concentrations detected in Withania somnifera (ashwagandha) by high performance liquid chromatography analysis, BMC Complement. Altern. Med., 11, 1 Sangwan, 2008, Withanolide A is inherently de novo biosynthesized in roots of the medicinal plant Ashwagandha (Withania somnifera), Physiol. Plant., 133, 278, 10.1111/j.1399-3054.2008.01076.x Vaishnavi, 2013, Dynamics of withanolide biosynthesis in relation to temporal expression pattern of metabolic genes in Withania somnifera (L.) Dunal: a comparative study in two morpho-chemovariants, Mol. Biol. Rep., 40, 7007, 10.1007/s11033-013-2820-z Nayak, 2009, HPTLC method for analysis of withaferin A in Ashwagandha (Withania somnifera), J. Planar Chromatogr. Mod. TLC., 22, 197, 10.1556/JPC.22.2009.3.7 Patel, 2014, Novel validated HPTLC method for estimation of withaferin A in polyherbal formulations, Anal. Methods, 6, 3009, 10.1039/c3ay41608a Jirge, 2011, Development and validation of a novel HPTLC method for simultaneous estimation of betasitosteroldglucoside and withaferin A, Int J Pharm Pharm Sci, 3, 227 Devkar, 2014, Estimation of antioxidant potential of individual components present in complex mixture of Withania somnifera (Ashwagandha) root fraction by thin-layerchromatography-2,2-diphenyl-1-picrylhdrazyl method, J. Planar Chromatogr. Mod. TLC., 27, 157, 10.1556/JPC.27.2014.3.2 Kushwaha, 2011, Standardization of ashwagandharishta formulation by TLC method, Int. J. ChemTech Res., 3, 1033 Ganzera, 2003, Quantitative HPLC analysis of withanolides in Withania somnifera, Fitoterapia, 74, 68, 10.1016/S0367-326X(02)00325-8 Uniyal, 2007, Analysis of withanolides in root and leaf ofWithania somnifera by HPLC with photodiode array and evaporative light scattering detection, Phytochem. Anal., 19, 148 Rajendra, 2010, HPLC estimation of withaferinA and boswellic acid in formulated gels, Int. J. Pharm. Biol. Archiv., 1, 183 Ali, 2010, RP-HPLC analysis of withanolides in the flowers, leaves, and roots of Withania somnifera, Acta Chromatogr., 22, 473, 10.1556/AChrom.22.2010.3.10 Rajasekar, 2011, Estimation of alkaloid content of Ashwagandha (Withania somnifera) with HPLC methods, Fitoterapia, 2, 39 Agrawal, 2015, Simultaneous estimation of withaferin A and Z-Guggulsterone in marketed formulation by RP-HPLC, J. Chromatogr. Sci., 53, 940, 10.1093/chromsci/bmu153 Khajuria, 2004, Separation, identification, and quantification of selected withanolides in plant extracts of Withania somnifera by HPLC-UV(DAD) - positive ion electrospray ionisation-mass spectrometry, J. Sep. Sci., 27, 541, 10.1002/jssc.200301690 Bolleddula, 2012, Identification of metabolites in Withania sominfera fruits by liquid chromatography and high-resolution mass spectrometry, Rapid Commun. Mass Spectrom., 26, 1277, 10.1002/rcm.6221 Chandra, 2015, Quality control assessment of polyherbal formulation based on a quantitative determination multimarker approach by ultra high performance liquid chromatography with tandem mass spectrometry using polarity switching combined with multivariate analysis, J. Sep. Sci., 38, 3183, 10.1002/jssc.201500458 Trivedi, 2017, Metabolite profiling in Withania somnifera roots hydroalcoholic extract using LC/MS, GC/MS and NMR spectroscopy, Chem. Biodivers., 14, 1, 10.1002/cbdv.201600280 ICH Topic Q2 B, 1996 Teotia, 2016, Development and validation of a new HPLC method for the determination of biphenyl and dibenzofuran phytoalexins in Rosaceae, J. Chromatogr. Sci., 54, 918, 10.1093/chromsci/bmw019 Patil, 2013, Determination of withaferin A and withanolide A in mice plasma using high-performance liquid chromatography-tandem mass spectrometry: application to pharmacokinetics after oral administration of Withania somnifera aqueous extract, J. Pharm. Biomed. Anal., 80, 203, 10.1016/j.jpba.2013.03.001