Differential effects of harmaline and ouabain on intestinal sodium, phenylalanine and β-methyl-glucoside transport

Naunyn-Schmiedebergs Archiv für Pharmakologie und experimentelle Pathologie - 1976
F. V. Sepúlveda1, M. Buclon1, J. W. L. Robinson1
1Département de Chirurgie Expérimentale, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland

Tóm tắt

Harmaline inhibits both the Na+-K+-ATPase activity and the uptake of l-phenylalanine in guinea-pig intestinal mucosa. The latter effect is not a direct consequence of the former, since higher concentrations are needed to inhibit the enzyme than the influx into the mucosa. Furthermore the uptake is still sensitive to harmaline when the Na+-K+-ATPase has been fully inhibited by ouabain. Harmaline can inhibit l-phenylalanine influx at a concentration at which it does not affect intracellular ion concentrations. Ouabain, however, inhibits the uptake of l-phenylalanine only after a 30 min preincubation period, when the intracellular sodium concentration reached the extracellular level. Harmaline also interferes with the influx of β-methyl-d-glucoside in the mucosa of the dog colon. Addition of harmaline at the mucosal face of the tissue suppresses all net transport of sodium and chloride ions and l-phenylalanine across the mucosa. Thus the same mode of action appears to apply in both the guinea-pig ileum and the dog colon.

Từ khóa


Tài liệu tham khảo

Alvarado, F., Crane, R. K.: Phlorizin as a competitive inhibitor of the active transport of sugars by the hamster small intestine in vitro. Biochim. biophys. Acta (Amst.) 56, 170–172 (1962)

Canessa, M., Jaimovich, E., de la Fuente, M.: Harmaline: a competitive inhibitor of Na ion in the (Na+-K+)-ATPase system. J. Membr. Biol. 13, 263–282 (1973)

Csáky, T. Z., Hara, Y.: Inhibition of active intestinal sugar transport by digitalis. Amer. J. Physiol. 209, 467–472 (1965)

Dunn, M. J., Hunt, W.: The effects of harmaline on sodium transport in human erythrocytes: Evidence in favor of an action at the interior sodium-sensitive sites. J. Pharmacol. exp. Ther. 193, 903–909 (1975)

Ehrenfeld, J., García-Romeu, F.: Action de l'harmaline sur le transport de sodium à travers la peau isolée de Rana esculenta et la branchie d'Astacus leptodactylus. C. R. Acad. Sci. (Paris) 281, 547–550 (1975)

Forth, W., Rummel, W.: Wirkung von Herzglykosiden auf Calcium-, Natrium-, Wasser-und Glukosetransport am isolierten Dünndarm. Helv. physiol. pharmacol. Acta 25, 8–23 (1967)

Lauterbach, F., Gerber, Ch., Nitz, D., Prescher, K.: Über Unterschiede im Mechanismus der enteralen Resorption kardiotoner Steroide und anderer Pharmaka. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 257, 432–457 (1967)

Lison, L.: Statistique appliquée à la biologie expérimentale, p. 169. Paris: Gauthier-Villars 1968

Luisier, A.-L., Robinson, J. W. L.: Inhibition of intestinal sugar and amino acid transport by n-butyl-biguanide. In: Comparative physiology (L. Bolis, K. Schmidt-Nielsen, and S. H. P. Maddrell, eds.), pp. 465–475. Amsterdam: North Holland 1973

Powell, D. W., Binder, H. J., Curran, P. F.: Electrolyte secretion by the guinea pig ileum in vitro. Amer. J. Physiol. 223, 531–537 (1972)

Robinson, J. W. L.: The difference in sensitivity to cardiac steroids of (Na++K+)-stimulated ATPase and amino acid transport in the intestinal mucosa of the rat and other species. J. Physiol. (Lond.) 206, 41–60 (1970)

Robinson, J. W. L.: Inhibition of transport processes in the dog colon. In: Intestinal ion transport (J. W. L. Robinson, ed.), pp. 287–298. Lancaster: Medical & Technical Publ. Co. 1976

Robinson, J. W. L., Felber, J.-P.: Compartments of the uptake of amino-acids by intestinal fragments during in vitro incubation. Gastroenterologia (Basel) 104, 335–342 (1965)

Robinson, J. W. L., Luisier, A.-L., Mirkovitch, V.: Transport of amino-acids and sugars by the dog colonic mucosa. Pflügers Arch. 345, 317–326 (1973)

Schultz, S. G., Zalusky, R.: Ion transport in isolated rabbit ileum. The interaction between active sodium and active sugar transport. J. gen. Physiol. 47, 1043–1059 (1964)

Schultz, S. G., Fuisz, R. E., Curran, P. F.: Amino acid and sugar transport in rabbit ileum. J. gen. Physiol. 49, 849–866 (1966)

Sepúlveda, F. V., Robinson, J. W. L.: Harmaline, a potent inhibitor of sodium-dependent transport. Biochim. biophys. Acta (Amst.) 373, 527–531 (1974)

Sepúlveda, F. V., Robinson, J. W. L.: New characteristics of harmaline inhibition of intestinal transport systems. Naunyn-Schmiedeberg's Arch. Pharmacol. 291, 201–212 (1975)

Sepúlveda, F. V., Robinson, J. W. L.: The influence of harmaline on sodium and sodium-dependent transport mechanisms. In: Intestinal ion transport (J. W. L. Robinson, ed.), pp. 157–170. Lancaster: Medical & Technical Publ. Co. 1976

Sepúlveda, F. V., Buclon, M., Robinson, J. W. L. L'harmaline, un inhibiteur des processus de transport intestinal associés aux mouvements des ions sodium. Gastroent. clin. biol. (sous presse, 1977)

Thông tin
Thông tin xuất bản

Naunyn-Schmiedebergs Archiv für Pharmakologie und experimentelle Pathologie

Thông tin tác giả