HspBP1 in Complex with the Peptide of the Innate Immunity Protein Tag7 is Able to Lyse Tumor Cells Carrying TNFR1 Receptor
Doklady Biochemistry and Biophysics - Trang 1-5 - 2024
Tóm tắt
The search for new cytotoxic agents capable of lysing tumor cells is an important task in the fight against cancer. Here we have shown that the HspBP1 protein, the chaperone of the heat shock protein Hsp70, is able to form a complex with the previously discovered peptide (17.1) of the innate immunity protein Tag7. Experiments using thermophoresis demonstrated that the affinity of the Tag7 protein peptide 17.1 to the HspBP1 molecule is 100 times higher than that of the full-sized Tag7 molecule. The addition of the 17.1–HspBP1 complex to tumor cells induces apoptosis and necroptosis in them. The results obtained in this work can be used to develop promising antitumor drugs.
Tài liệu tham khảo
Carswell, E.A. et al., An endotoxin-induced serum factor that causes necroptosis of tumors, Proc. Natl. Acad. Sci. U. S. A., 1975, vol. 72, pp. 3666–3670.
Siegmund D., and Wajant H., TNF and TNF receptors as therapeutic targets for rheumatic diseases and beyond, Nat. Rev. Rheumatol., 2023, vol. 19, pp. 576–591.
Huyghe, J., Priem, D., and Bertrand, M.J.M., Cell death checkpoints in the TNF pathway, Trends Immunol., 2023, vol. 44, pp. 628–643.
Shi, G. and Hu, Y., TNFR1 and TNFR2, which link NF-κB activation, drive lung cancer progression, cell dedifferentiation, and metastasis, Cancers (Basel), 2023, vol. 15, p. 4299.
Gough, P. and Myles, I.A., Tumor necroptosis factor receptors: pleiotropic signaling complexes and their differential effects, Front. Immunol., 2020, vol. 11, p. 585880.
Ashkenazi, A. and Salvesen, G., Regulated cell death: signaling and mechanism, Annu. Rev. Cell Dev. Biol., 2014, vol. 30, pp. 337–356.
Roberts, J.Z., Crawford, N., and Longley, D.B., The role of ubiquitination in apoptosis and necroptosis, Cell Death Differ., 2022, vol. 29, pp. 272–284.
Kustikova, O.S. et al., Cloning of the tag7 gene expressed in metastatic mouse tumors, Genetika, 1996, vol. 32, pp. 621–628.
Yashin, D.V. et al., Tag7 (PGLYRP1) in complex with Hsp70 induces alternative cytotoxic processes in tumor cells via TNFR1 receptor, J. Biol. Chem., 2015, vol. 290, pp. 21724–21731.
Yashin, D.V. et al., The Tag7-Hsp70 cytotoxic complex induces tumor cell necroptosis via permeabilization of lysosomes and mitochondria, Biochimie, 2016, vol. 123, pp. 32–36.
Yashin, D.V. et al., The heat shock-binding protein (HspBP1) protects cells against the cytotoxic action of the Tag7-Hsp70 complex, J. Biol. Chem., 2011, vol. 286, pp. 10258–10264.
Bracher, A. and Verghese, J., Nucleotide exchange factors for Hsp70 molecular chaperones: GrpE, Hsp110/Grp170, HspBP1/Sil1, and BAG domain proteins, Subcell Biochem., 2023, vol. 101, pp. 1–39.
Yurkina, D.M. et al., Short peptides of innate immunity protein Tag7 (PGLYRP1) selectively induce inhibition or activation of tumor cell death via TNF receptor, Int. J. Mol. Sci., 2023, vol. 24, p. 11363.