An evaluation of porcine epidemic diarrhea virus survival in individual feed ingredients in the presence or absence of a liquid antimicrobial
Tóm tắt
Contaminated complete feed and porcine plasma are risk factors for
PEDV introduction to farms and a liquid antimicrobial has been proven useful for
reducing risk. This study provides information on the survivability of PEDV
across common swine feed ingredients in the presence or absence of the liquid
antimicrobial. Eighteen ingredients commonly included in commercial swine diets
were selected, including 3 grain sources (corn, soybean meal (SBM), dried
distillers grains with solubles (DDGS)), 5 porcine by-products (spray-dried
plasma, purified plasma, intestinal mucosa, meat and bone meal and red blood
cells (RBCs)), 3 vitamin/trace mineral (VTM) mixes (sow, nursery, finishing), 2
fat sources (choice white grease and soy oil), 3 synthetic amino acids (lysine
HCL, D/L methionine, threonine), as well as limestone and dry choline chloride.
Complete feed and stock PEDV served as controls. Thirty grams of each ingredient
were inoculated with 2 mL PEDV. A matched set of samples were treated with the
formaldehyde-based liquid antimicrobial SalCURB® (LA). All samples (n = 320) were stored outdoors under winter time
ambient conditions for 30 days. Samples were submitted on 1, 7, 14 and 30 days
post-inoculation (DPI) and tested by PCR and virus isolation (VI). All
VI-negative samples were tested by swine bioassay. Viable PEDV was detected by
VI or swine bioassay at 1, 7, 14 and 30 DPI from SBM, DDGS, meat & bone
meal, RBCs, lysine HCL, D/L methionine, choice white grease, choline chloride,
complete feed and stock virus control and at 7 DPI in limestone and at 14 DPI in
threonine. Supplementary testing of complete feed and SBM indicated viable virus
out to 45 and 180 DPI, respectively. All other samples were negative by VI and
bioassay. In contrast, treatment with LA inactivated PEDV across all ingredients
on 1 DPI and induced RNA reduction over time. Under the conditions of this study, PEDV viability in feed was
influenced by ingredient with extended survival in SBM. Furthermore, LA
treatment rendered virus inactive, independent of ingredient type.
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