Microstratigraphic preservation of ancient faunal and hominin DNA in Pleistocene cave sediments

Proceedings of the National Academy of Sciences of the United States of America - Tập 119 Số 1 - 2022
Diyendo Massilani1, Mike W. Morley2, Susan M. Mentzer3,4, Vera Aldeias5, Benjamin Vernot1, Christopher E. Miller4,6,6, Mareike Stahlschmidt7, М.B. Kozlikin8, M.V. Shunkov8, А. П. Деревянко8, Nicholas J. Conard3,4, Sarah Wurz9,6, Christopher S. Henshilwood9,6, Javi Vasquez10, Elena Essel1, Sarah Nagel1, Julia Richter1, Birgit Nickel1, Richard G. Roberts11,12, Svante Pääbo1, Viviane Slon13,1,14,15, Paul Goldberg12,12, Matthias Meyer1
1Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, D-04103 Leipzig, Germany;
2Archaeology, College of Humanities, Arts and Social Sciences, Flinders University, Adelaide, South Australia 5042, Australia;
3Institute for Archaeological Sciences, Eberhard Karls University of Tübingen, Tübingen, 72074 Tübingen, Germany;
4Senckenberg Centre for Human Evolution and Paleoenvironment, Universität Tübingen, Tübingen, 72070 Tübingen, Germany;
5Interdisciplinary Center for Archaeology and Evolution of Human Behaviour, University of Algarve, 8005-139 Faro, Portugal;
6Sentre For Fremragende Forskning (SFF), Centre for Early Sapiens Behaviour, University of Bergen, Bergen, 5020 Bergen, Norway;
7Department of Human Evolution, Max Planck Institute for Evolutionary Anthropology, Leipzig, D-04103 Leipzig, Germany;
8Institute of Archaeology and Ethnography, Russian Academy of Sciences, Siberian Branch, Novosibirsk 630090, Russia;
9School of Geography, Archaeology and Environmental Studies and Evolutionary Studies Institute, University of the Witwatersrand, Johannesburg, Wits 2050, South Africa;
10Center for Environmental Management of Military Lands, Colorado State University, Fort Collins, CO 80524;
11Australian Research Council Centre of Excellence for Australian Biodiversity and Heritage, University of Wollongong, Wollongong, New South Wales 2522, Australia;
12Centre for Archaeological Science, School of Earth, Atmospheric and Life Sciences, University of Wollongong, Wollongong, New South Wales 2522, Australia
13Department of Anatomy and Anthropology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 6997801, Israel
14Department of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 6997801, Israel;
15The Dan David Center for Human Evolution and Biohistory Research, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 6997801, Israel

Tóm tắt

Significance DNA preserved in sediments has emerged as an important source of information about past ecosystems, independent of the discovery of skeletal remains. However, little is known about the sources of sediment DNA, the factors affecting its long-term preservation, and the extent to which it may be translocated after deposition. Here, we show that impregnated blocks of intact sediment are excellent archives of DNA. DNA distribution is highly heterogeneous at the microscale in the cave sediment we studied, suggesting that postdepositional movement of DNA is unlikely to be a common phenomenon in cases where the stratigraphy is undisturbed. Combining micromorphological analysis with microstratigraphic retrieval of ancient DNA therefore allows genetic information to be associated with the detailed archaeological and ecological record preserved in sediments.

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Proceedings of the National Academy of Sciences of the United States of America

Tập 119 Số 1

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