Brevican distinctively assembles extracellular components at the large diameter nodes of Ranvier in the CNS

Journal of Neurochemistry - Tập 108 Số 5 - Trang 1266-1276 - 2009
Yoko Bekku1, Uwe Rauch2, Yoshifumi Ninomiya3, Toshitaka Oohashi3
1Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan
2Vessel Wall Biology, Institute for Experimental Medical Sciences, Lund University, Lund, Sweden
3Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan

Tóm tắt

AbstractBrevican is known to be an abundant extracellular matrix component in the adult brain and a structural constituent of perineuronal nets. We herein show that brevican, tenascin‐R (TN‐R) and phosphacan are present at the nodes of Ranvier on myelinated axons with a particularly large diameter in the central nervous system. A brevican deficiency resulted in a reorganization of the nodal matrices, which was characterized by the shift of TN‐R, and concomitantly phosphacan, from an axonal diameter‐dependent association with nodes to an axonal diameter independent association. Supported by the co‐immunoprecipitation results, these observations indicate that the presence of TN‐R and phosphacan at nodes is normally brevican‐dependent, while in the absence of brevican these molecules can also be recruited by versican V2. The versican V2 and Bral1 distribution was not affected, thus indicating a brevican‐independent role of these two molecules for establishing hyaluronan‐binding matrices at the nodes. Our results revealed that brevican plays a crucial role in determining the specialization of the hyaluronan‐binding nodal matrix assemblies in large diameter nodes.

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Thông tin
Thông tin xuất bản

Journal of Neurochemistry

Tập 108 Số 5

1266-1276

Thông tin tác giả