Nedd4 and Nedd4-2: closely related ubiquitin-protein ligases with distinct physiological functions
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Ciechanover A . Proteolysis: From the lysosome to ubiquitin and the proteasome. Nat Rev Mol Cell Biol 2005; 6: 79–87.
Xu P, Duong DM, Seyfried NT, Cheng D, Xie Y, Robert J et al. Quantitative proteomics reveals the function of unconventional ubiquitin chains in proteasomal degradation. Cell 2009; 137: 133–145.
Rotin D, Kumar S . Physiological functions of the HECT family of ubiquitin ligases. Nat Rev Mol Cell Biol 2009; 10: 398–409.
Huibregtse JM, Scheffner M, Beaudenon S, Howley PM . A family of proteins structurally and functionally related to the E6-AP ubiquitin-protein ligase. Proc Natl Acad Sci USA 1995; 92: 2563–2567.
Kumar S, Tomooka Y, Noda M . Identification of a set of genes with developmentally down-regulated expression in the mouse brain. Biochem Biophys Res Commun 1992; 185: 1155–1161.
Hein C, Springael JY, Volland C, Haguenauer-Tsapis R, Andre B . NPl1, an essential yeast gene involved in induced degradation of Gap1 and Fur4 permeases, encodes the Rsp5 ubiquitin-protein ligase. Mol Microbiol 1995; 18: 77–87.
Matentzoglu K, Scheffner M . Ubiquitin ligase E6-AP and its role in human disease. Biochem Soc Trans 2008; 36 (Pt 5): 797–801.
Scheffner M, Huibregtse JM, Vierstra RD, Howley PM . The HPV-16 E6 and E6-AP complex functions as a ubiquitin-protein ligase in the ubiquitination of p53. Cell 1993; 75: 495–505.
Huang L, Kinnucan E, Wang G, Beaudenon S, Howley PM, Huibregtse JM et al. Structure of an E6AP-UbcH7 complex: Insights into ubiquitination by the E2-E3 enzyme cascade. Science 1999; 286: 1321–1326.
Harvey KF, Kumar S . Nedd4-like proteins: An emerging family of ubiquitin-protein ligases implicated in diverse cellular functions. Trends Cell Biol 1999; 9: 166–169.
Ingham RJ, Gish G, Pawson T . The Nedd4 family of E3 ubiquitin ligases: Functional diversity within a common modular architecture. Oncogene 2004; 23: 1972–1984.
Shearwin-Whyatt L, Dalton HE, Foot N, Kumar S . Regulation of functional diversity within the Nedd4 family by accessory and adaptor proteins. Bioessays 2006; 28: 617–628.
Fotia AB, Cook DI, Kumar S . The ubiquitin-protein ligases Nedd4 and Nedd4–2 show similar ubiquitin-conjugating enzyme specificities. Int J Biochem Cell Biol 2006; 38: 472–479.
Kumar S, Harvey KF, Kinoshita M, Copeland NG, Noda M, Jenkins NA . cDNA cloning, expression analysis, and mapping of the mouse Nedd4 gene. Genomics 1997; 40: 435–443.
Harvey KF, Dinudom A, Komwatana P, Jolliffe CN, Day ML, Parasivam G et al. All three WW domains of murine Nedd4 are involved in the regulation of epithelial sodium channels by intracellular Na+. J Biol Chem 1999; 274: 12525–12530.
Plant PJ, Lafont F, Lecat S, Verkade P, Simons K, Rotin D . Apical membrane targeting of Nedd4 is mediated by an association of its C2 domain with annexin XIIIb. J Cell Biol 2000; 149: 1473–1484.
Fotia AB, Dinudom A, Shearwin KE, Koch JP, Korbmacher C, Cook DI et al. The role of individual Nedd4–2 (KIAA0439) WW domains in binding and regulating epithelial sodium channels. FASEB J 2003; 17: 70–72.
Kanelis V, Bruce MC, Skrynnikov NR, Rotin D, Forman-Kay JD . Structural determinants for high-affinity binding in a Nedd4 WW3* domain-Comm PY motif complex. Structure 2006; 14: 543–553.
Itani OA, Campbell JR, Herrero J, Snyder PM, Thomas CP . Alternate promoters and variable splicing lead to hNedd4–2 isoforms with a C2 domain and varying number of WW domains. Am J Physiol Renal Physiol 2003; 285: F916–F929.
Itani OA, Stokes JB, Thomas CP . Nedd4–2 isoforms differentially associate with ENaC and regulate its activity. Am J Physiol Renal Physiol 2005; 289: F334–F346.
Staub O, Dho S, Henry P, Correa J, Ishikawa T, McGlade J et al. WW domains of Nedd4 bind to the proline-rich PY motifs in the epithelial Na+ channel deleted in Liddle's syndrome. EMBO J 1996; 15: 2371–2380.
Harvey KF, Dinudom A, Cook DI, Kumar S . The Nedd4-like protein KIAA0439 is a potential regulator of the epithelial sodium channel. J Biol Chem 2001; 276: 8597–8601.
Kamynina E, Debonneville C, Bens M, Vandewalle A, Staub O . A novel mouse Nedd4 protein suppresses the activity of the epithelial Na+ channel. FASEB J 2001; 15: 204–214.
Snyder PM, Steines JC, Olson DR . Relative contribution of Nedd4 and Nedd4–2 to ENaC regulation in epithelia determined by RNA interference. J Biol Chem 2004; 279: 5042–5046.
Morrione A, Plant P, Valentinis B, Staub O, Kumar S, Rotin D et al. mGrb10 Interacts with Nedd4. J Biol Chem 1999; 274: 24094–24099.
Katz M, Shtiegman K, Tal-Or P, Yakir L, Mosesson Y, Harari D et al. Ligand-independent degradation of epidermal growth factor receptor involves receptor ubiquitylation and Hgs, an adaptor whose ubiquitin-interacting motif targets ubiquitylation by Nedd4. Traffic 2002; 3: 740–751.
Murdaca J, Treins C, Monthouel-Kartmann MN, Pontier-Bres R, Kumar S, Van Obberghen E et al. Grb10 prevents Nedd4-mediated vascular endothelial growth factor receptor-2 degradation. J Biol Chem 2004; 279: 26754–26761.
Peruzzi F, Prisco M, Morrione A, Valentinis B, Baserga R . Anti-apoptotic signaling of the insulin-like growth factor-I receptor through mitochondrial translocation of c-Raf and Nedd4. J Biol Chem 2001; 276: 25990–25996.
Cao XR, Lill NL, Boase N, Shi PP, Croucher DR, Shan H et al. Nedd4 controls animal growth by regulating IGF-1 signaling. Sci Signal 2008; 1: ra5.
Fouladkou F, Landry T, Kawabe H, Neeb A, Lu C, Brose N et al. The ubiquitin ligase Nedd4–1 is dispensable for the regulation of PTEN stability and localization. Proc Natl Acad Sci USA 2008; 105: 8585–8590.
Vecchione A, Marchese A, Henry P, Rotin D, Morrione A . The Grb10/Nedd4 complex regulates ligand-induced ubiquitination and stability of the insulin-like growth factor I receptor. Mol Cell Biol 2003; 23: 3363–3372.
Girnita L, Girnita A, Larsson O . Mdm2-dependent ubiquitination and degradation of the insulin-like growth factor 1 receptor. Proc Natl Acad Sci USA 2003; 100: 8247–8252.
Sehat B, Andersson S, Girnita L, Larsson O . Identification of c-Cbl as a new ligase for insulin-like growth factor-I receptor with distinct roles from Mdm2 in receptor ubiquitination and endocytosis. Cancer Res 2008; 68: 5669–5677.
Heissmeyer V, Macian F, Im SH, Varma R, Feske S, Venuprasad K et al. Calcineurin imposes T cell unresponsiveness through targeted proteolysis of signaling proteins. Nat Immunol 2004; 5: 255–265.
Fang D, Elly C, Gao B, Fang N, Altman Y, Joazeiro C et al. Dysregulation of T lymphocyte function in itchy mice: A role for Itch in TH2 differentiation. Nat Immunol 2002; 3: 281–287.
Yang B, Gay DL, MacLeod MKL, Cao X, Sweezer EM, Kappler J et al. Nedd4 augments the adaptive immune response by promoting ubiquitin mediated degradation of Cbl-b in activated T cells. Nat Immunol 2008; 9: 1356–1363.
Perry WL, Hustad CM, Swing DA, O’Sullivan TN, Jenkins NA, Copeland NG . The itchy locus encodes a novel ubiquitin protein ligase that is disrupted in a18 H mice. Nat Genet 1998; 18: 143–146.
Magnifico A, Ettenberg S, Yang C, Mariano J, Tiwari S, Fang S et al. WW domain HECT E3s target Cbl RING finger E3s for proteasomal degradation. J Biol Chem 2003; 278: 43169–43177.
Liu Y, Oppenheim RW, Sugiura Y, Lin W . Abnormal development of the neuromuscular junction in Nedd4-deficient mice. Dev Biol 2009; 330: 153–166.
Giorgetti-Peraldi S, Murdaca J, Mas JC, Van Obberghen E . The adapter protein, Grb10, is a positive regulator of vascular endothelial growth factor signaling. Oncogene 2001; 20: 3959–3968.
Wang X, Trotman LC, Koppie T, Alimonti A, Chen Z, Gao Z et al. NEDD4–1 is a proto-oncogenic ubiquitin ligase for PTEN. Cell 2007; 128: 129–139.
Trotman LC, Wang X, Alimonti A, Chen Z, Teruya-Feldstein J, Yang H et al. Ubiquitination regulates PTEN nuclear import and tumor suppression. Cell 2007; 128: 141–156.
Dimmock NJ . Introduction to Modern Virology. 6th edn. Blackwell Science: Oxford, Cambridge, MA, USA, 2007.
Ikeda M, Ikeda A, Longan LC, Longnecker R . The Epstein-Barr virus latent membrane protein 2A PY motif recruits WW domain-containing ubiquitin-protein ligases. Virology 2000; 268: 178–191.
Strack B, Calistri A, Accola MA, Palu G, Gottlinger HG . A role for ubiquitin ligase recruitment in retrovirus release. Proc Natl Acad Sci USA 2000; 97: 13063–13068.
Harty RN, Brown ME, Wang G, Huibregtse J, Hayes FP . A PPxY motif within the VP40 protein of Ebola virus interacts physically and functionally with a ubiquitin ligase: Implications for filovirus budding. Proc Natl Acad Sci USA 2000; 97: 13871–13876.
Pradervand S, Vandewalle A, Bens M, Gautschi I, Loffing J, Hummler E et al. Dysfunction of the epithelial sodium channel expressed in the kidney of a mouse model for Liddle syndrome. J Am Soc Nephrol 2003; 14: 2219–2228.
Shi PP, Cao XR, Sweezer EM, Kinney TS, Williams NR, Husted RF et al. Salt-sensitive hypertension and cardiac hypertrophy in mice deficient in the ubiquitin ligase Nedd4–2. Am J Physiol Renal Physiol 2008; 295: F462–F470.
Pradervand S, Wang Q, Burnier M, Beermann F, Horisberger JD, Hummler E et al. A mouse model for Liddle's syndrome. J Am Soc Nephrol 1999; 10: 2527–2533.
Fotia AB, Ekberg J, Adams DJ, Cook DI, Poronnik P, Kumar S . Regulation of neuronal voltage-gated sodium channels by the ubiquitin-protein ligases Nedd4 and Nedd4–2. J Biol Chem 2004; 279: 28930–28935.
Rougier JS, van Bemmelen MX, Bruce MC, Jespersen T, Gavillet B, Apotheloz F et al. Molecular determinants of voltage-gated sodium channel regulation by the Nedd4/Nedd4-like proteins. Am J Physiol Cell Physiol 2005; 288: C692–C701.
van Bemmelen MX, Rougier JS, Gavillet B, Apotheloz F, Daidie D, Tateyama M et al. Cardiac voltage-gated sodium channel Nav1.5 is regulated by Nedd4–2 mediated ubiquitination. Circ Res 2004; 95: 284–291.
Brickley DR, Mikosz CA, Hagan CR, Conzen SD . Ubiquitin modification of serum and glucocorticoid-induced protein kinase-1 (SGK-1). J Biol Chem 2002; 277: 43064–43070.
Zhou R, Snyder PM . Nedd4–2 phosphorylation induces serum and glucocorticoid-regulated kinase (SGK) ubiquitination and degradation. J Biol Chem 2005; 280: 4518–4523.
Kotorashvili A, Russo SJ, Mulugeta S, Guttentag SH, Beers MF . Anterograde transport of surfactant protein C proprotein to distal processing compartments requires PPDY mediated association with NEDD4 ubiquitin ligases. J Biol Chem 2009; 284: 16667–16678.
Conkright J, Apsley KS, Martin E, Ridsdale R, Rice WR, Cheng-Lun Na C-L et al. Nedd4–2 mediated ubiquitination facilitates processing of surfactant protein C. Am J Resp Cell Mol 2009. in press.
Farrell PM, Avery ME . Hyaline membrane disease. Am Rev Respir Dis 1975; 111: 657–688.
Nogee LM, Dunbar 3rd AE, Wert SE, Askin F, Hamvas A, Whitsett JA . A mutation in the surfactant protein C gene associated with familial interstitial lung disease. N Engl J Med 2001; 344: 573–579.
Hettema EH, Valdez-Taubas J, Pelham HR . Bsd2 binds the ubiquitin ligase Rsp5 and mediates the ubiquitination of transmembrane proteins. EMBO J 2004; 23: 1279–1288.
Liu XF, Supek F, Nelson N, Culotta VC . Negative control of heavy metal uptake by the Saccharomyces cerevisiae BSD2 gene. J Biol Chem 1997; 272: 11763–11769.
Stimpson HE, Lewis MJ, Pelham HR . Transferrin receptor-like proteins control the degradation of a yeast metal transporter. EMBO J 2006; 25: 662–672.
Jolliffe CN, Harvey KF, Haines BP, Parasivam G, Kumar S . Identification of multiple proteins expressed in murine embryos as binding partners for the WW domains of the ubiquitin-protein ligase nedd4. Biochem J 2000; 351 (Pt 3): 557–565.
Harvey KF, Shearwin-Whyatt LM, Fotia A, Parton RG, Kumar S . N4WBP5, a potential target for ubiquitination by the Nedd4 family of proteins, is a novel Golgi-associated protein. J Biol Chem 2002; 277: 9307–9317.
Konstas AA, Shearwin-Whyatt LM, Fotia AB, Degger B, Riccardi D, Cook DI et al. Regulation of the epithelial sodium channel by N4WBP5A, a novel Nedd4/Nedd4–2–interacting protein. J Biol Chem 2002; 277: 29406–29416.
Shearwin-Whyatt LM, Brown DL, Wylie FG, Stow JL, Kumar S . N4WBP5A (Ndfip2), a Nedd4-interacting protein, localizes to multivesicular bodies and the golgi, and has a potential role in protein trafficking. J Cell Sci 2004; 117 (Pt 16): 3679–3689.
Oliver PM, Cao X, Worthen GS, Shi P, Briones N, MacLeod M et al. Ndfip1 protein promotes the function of itch ubiquitin ligase to prevent T cell activation and T helper 2 cell-mediated inflammation. Immunity 2006; 25: 929–940.
Foot NJ, Dalton HE, Shearwin-Whyatt LM, Dorstyn L, Tan SS, Yang B et al. Regulation of the divalent metal ion transporter DMT1 and iron homeostasis by a ubiquitin-dependent mechanism involving Ndfips and WWP2. Blood 2008; 112: 4268–4275.
Sang Q, Kim MH, Kumar S, Bye N, Morganti-Kossman MC, Gunnersen J et al. Nedd4-WW domain-binding protein 5 (Ndfip1) is associated with neuronal survival after acute cortical brain injury. J Neurosci 2006; 26: 7234–7244.
Putz U, Howitt J, Lackovic J, Foot N, Kumar S, Silke J et al. Nedd4 family-interacting protein 1 (Ndfip1) is required for the exosomal secretion of Nedd4 family proteins. J Biol Chem 2008; 283: 32621–32627.
Bridges D, Moorhead GB . 14–3–3 proteins: A number of functions for a numbered protein. Sci STKE 2005; 2005: re10.
Bhalla V, Daidie D, Li H, Pao AC, LaGrange LP, Wang J et al. Serum- and glucocorticoid-regulated kinase 1 regulates ubiquitin ligase neural precursor cell-expressed, developmentally down-regulated protein 4–2 by inducing interaction with 14–3–3. Mol Endocrinol 2005; 19: 3073–3084.
Ichimura T, Yamamura H, Sasamoto K, Tominaga Y, Taoka M, Kakiuchi K et al. 14–3–3 proteins modulate the expression of epithelial Na+ channels by phosphorylation-dependent interaction with Nedd4–2 ubiquitin ligase. J Biol Chem 2005; 280: 13187–13194.
Debonneville C, Flores SY, Kamynina E, Plant PJ, Tauxe C, Thomas MA et al. Phosphorylation of Nedd4–2 by Sgk1 regulates epithelial Na(+) channel cell surface expression. EMBO J 2001; 20: 7052–7059.
Snyder PM, Olson DR, Thomas BC . Serum and glucocorticoid-regulated kinase modulates Nedd4–2–mediated inhibition of the epithelial Na+ channel. J Biol Chem 2002; 277: 5–8.
Liang X, Peters KW, Butterworth MB, Frizzell RA . 14–3–3 isoforms are induced by aldosterone and participate in its regulation of epithelial sodium channels. J Biol Chem 2006; 281: 16323–16332.
Nagaki K, Yamamura H, Shimada S, Saito T, Hisanaga SI, Taoka M et al. 14–3–3 mediates phosphorylation-dependent inhibition of the interaction between the ubiquitin E3 ligase Nedd4–2 and epithelial Na(+) channels. Biochemistry 2006; 45: 6733–6740.
Lee IH, Dinudom A, Sanchez-Perez A, Kumar S, Cook DI . Akt mediates the effect of insulin on epithelial sodium channels by inhibiting Nedd4–2. J Biol Chem 2007; 282: 29866–29873.
Liang X, Butterworth MB, Peters KW, Walker WH, Frizzell RA . An obligatory heterodimer of 14–3–3beta and 14–3–3epsilon is required for aldosterone regulation of the epithelial sodium channel. J Biol Chem 2008; 283: 27418–27425.
Edinger RS, Lebowitz J, Li H, Alzamora R, Wang H, Johnson JP et al. Functional regulation of the epithelial Na+ channel by I{kappa}B kinase-{beta} occurs via phosphorylation of the ubiquitin ligase Nedd4–2. J Biol Chem 2009; 284: 150–157.
Malakhova OA, Zhang DE . ISG15 inhibits Nedd4 ubiquitin E3 activity and enhances the innate antiviral response. J Biol Chem 2008; 283: 8783–8787.
Bruce C, Kanelis V, Fouladkou F, Debonneville A, Staub O, Rotin D . Regulation of Nedd4–2 self-ubiquitylation and stability by a PY motif located within its HECT-domain. Biochem J 2008; 415: 155–163.
Dieter M, Palmada M, Rajamanickam J, Aydin A, Busjahn A, Boehmer C et al. Regulation of glucose transporter SGLT1 by ubiquitin ligase Nedd4–2 and kinases SGK1, SGK3, and PKB. Obes Res 2004; 12: 862–870.
Asher C, Sinha I, Garty H . Characterization of the interactions between Nedd4–2, ENaC, and sgk-1 using surface plasmon resonance. Biochim Biophys Acta 2003; 1612: 59–64.
Dephoure N, Zhou C, Villen J, Beausoleil SA, Bakalarski CE, Elledge SJ et al. A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci USA 2008; 105: 10762–10767.
Angrand PO, Segura I, Volkel P, Ghidelli S, Terry R, Brajenovic M et al. Transgenic mouse proteomics identifies new 14–3–3–associated proteins involved in cytoskeletal rearrangements and cell signaling. Mol Cell Proteomics 2006; 5: 2211–2227.
Harvey KF, Harvey NL, Michael JM, Parasivam G, Waterhouse N, Alnemri ES et al. Caspase-mediated cleavage of the ubiquitin-protein ligase Nedd4 during apoptosis. J Biol Chem 1998; 273: 13524–13530.
Leon S, Erpapazoglou Z, Haguenauer-Tsapis R . Ear1p and Ssh4p are new adaptors of the ubiquitin ligase Rsp5p for cargo ubiquitylation and sorting at multivesicular bodies. Mol Biol Cell 2008; 19: 2379–2388.
Lin CH, MacGurn JA, Chu T, Stefan CJ, Emr SD . Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface. Cell 2008; 135: 714–725.
Nikko E, Sullivan JA, Pelham HR . Arrestin-like proteins mediate ubiquitination and endocytosis of the yeast metal transporter Smf1. EMBO Rep 2008; 9: 1216–1221.
Shenoy SK, Xiao K, Venkataramanan V, Snyder PM, Freedman NJ, Weissman AM . Nedd4 mediates agonist-dependent ubiquitination, lysosomal targeting, and degradation of the beta2-adrenergic receptor. J Biol Chem 2008; 283: 22166–22176.
Lafont F, Simons K . Raft-partitioning of the ubiquitin ligases Cbl and Nedd4 upon IgE-triggered cell signaling. Proc Natl Acad Sci USA 2001; 98: 3180–3184.
Leykauf K, Salek M, Bomke J, Frech M, Lehmann WD, Durst M et al. Ubiquitin protein ligase Nedd4 binds to connexin43 by a phosphorylation-modulated process. J Cell Sci 2006; 119 (Pt 17): 3634–3642.
Aoh QL, Castle AM, Hubbard CH, Katsumata O, Castle JD . SCAMP3 negatively regulates EGFR degradation and promotes receptor recycling. Mol Biol Cell 2009; 20: 1816–1832.
Dinudom A, Harvey KF, Komwatana P, Young JA, Kumar S, Cook DI . Nedd4 mediates control of an epithelial Na+ channel in salivary duct cells by cytosolic Na+. Proc Natl Acad Sci USA 1998; 95: 7169–7173.
Rost M, Doring T, Prange R . Gamma 2-adaptin, a ubiquitin-interacting adaptor, is a substrate to coupled ubiquitination by the ubiquitin ligase Nedd4 and functions in the endosomal pathway. J Biol Chem 2008; 283: 32119–32130.
Plant PJ, Correa J, Goldenberg NM, Bain J, Batt JA . The inositol phosphatase MTMR4 is a novel target of the ubiquitin ligase Nedd4. Biochem J 2009; 419: 57–63.
Koncarevic A, Jackman RW, Kandarian SC . The ubiquitin-protein ligase Nedd4 targets Notch1 in skeletal muscle and distinguishes the subset of atrophies caused by reduced muscle tension. FASEB J 2007; 21: 427–437.
Fedoroff OY, Townson SA, Golovanov AP, Baron M, Avis JM . The structure and dynamics of tandem WW domains in a negative regulator of notch signaling, Suppressor of deltex. J Biol Chem 2004; 279: 34991–35000.
Anindya R, Aygun O, Svejstrup JQ . Damage-induced ubiquitylation of human RNA polymerase II by the ubiquitin ligase Nedd4, but not Cockayne syndrome proteins or BRCA1. Mol Cell 2007; 28: 386–397.
Moren A, Imamura T, Miyazono K, Heldin CH, Moustakas A . Degradation of the tumor suppressor Smad4 by WW and HECT-domain ubiquitin ligases. J Biol Chem 2005; 280: 22115–22123.
Al Sorkhy M, Craig R, Market B, Ard R, Porter LA . The cyclin-dependent kinase activator, Spy1A, is targeted for degradation by the ubiquitin ligase NEDD4. J Biol Chem 2009; 284: 2617–2627.
Chan W, Tian R, Lee YF, Sit ST, Lim L, Manser E . Down-regulation of active ACK1 is mediated by association with the E3 ubiquitin ligase Nedd4–2. J Biol Chem 2009; 284: 8185–8194.
Hatanaka T, Hatanaka Y, Setou M . Regulation of amino acid transporter ATA2 by ubiquitin ligase Nedd4–2. J Biol Chem 2006; 281: 35922–35930.
Hryciw DH, Ekberg J, Lee A, Lensink IL, Kumar S, Guggino WB et al. Nedd4–2 functionally interacts with ClC-5: Involvement in constitutive albumin endocytosis in proximal tubule cells. J Biol Chem 2004; 279: 54996–55007.
Embark HM, Bohmer C, Palmada M, Rajamanickam J, Wyatt AW, Wallisch S et al. Regulation of CLC-Ka/barttin by the ubiquitin ligase Nedd4–2 and the serum- and glucocorticoid-dependent kinases. Kidney Int 2004; 66: 1918–1925.
Sorkina T, Miranda M, Dionne KR, Hoover BR, Zahniser NR, Sorkin A . RNA interference screen reveals an essential role of Nedd4–2 in dopamine transporter ubiquitination and endocytosis. J Neurosci 2006; 26: 8195–8205.
Boehmer C, Palmada M, Rajamanickam J, Schniepp R, Amara S, Lang F . Post-translational regulation of EAAT2 function by co-expressed ubiquitin ligase Nedd4–2 is impacted by SGK kinases. J Neurochem 2006; 97: 911–921.
Boehmer C, Henke G, Schniepp R, Palmada M, Rothstein JD, Broer S et al. Regulation of the glutamate transporter EAAT1 by the ubiquitin ligase Nedd4–2 and the serum and glucocorticoid-inducible kinase isoforms SGK1/3 and protein kinase B. J Neurochem 2003; 86: 1181–1188.
Ekberg J, Schuetz F, Boase NA, Conroy SJ, Manning J, Kumar S et al. Regulation of the voltage-gated K(+) channels KCNQ2/3 and KCNQ3/5 by ubiquitination. Novel role for Nedd4–2. J Biol Chem 2007; 282: 12135–12142.
Jespersen T, Membrez M, Nicolas CS, Pitard B, Staub O, Olesen SP et al. The KCNQ1 potassium channel is down-regulated by ubiquitylating enzymes of the Nedd4/Nedd4-like family. Cardiovasc Res 2007; 74: 64–74.
Henke G, Maier G, Wallisch S, Boehmer C, Lang F . Regulation of the voltage gated K+ channel Kv1.3 by the ubiquitin ligase Nedd4–2 and the serum and glucocorticoid inducible kinase SGK1. J Cell Physiol 2004; 199: 194–199.
Baltaev R, Strutz-Seebohm N, Korniychuk G, Myssina S, Lang F, Seebohm G . Regulation of cardiac shal-related potassium channel Kv 4.3 by serum- and glucocorticoid-inducible kinase isoforms in Xenopus oocytes. Pflugers Arch 2005; 450: 26–33.
Palmada M, Dieter M, Speil A, Bohmer C, Mack AF, Wagner HJ et al. Regulation of intestinal phosphate cotransporter NaPi IIb by ubiquitin ligase Nedd4–2 and by serum- and glucocorticoid-dependent kinase 1. Am J Physiol Gastrointest Liver Physiol 2004; 287: G143–G150.
Kuratomi G, Komuro A, Goto K, Shinozaki M, Miyazawa K, Miyazono K et al. NEDD4–2 (neural precursor cell expressed, developmentally down-regulated 4–2) negatively regulates TGF-beta (transforming growth factor-beta) signalling by inducing ubiquitin-mediated degradation of Smad2 and TGF-beta type I receptor. Biochem J 2005; 386 (Pt 3): 461–470.
Arevalo JC, Waite J, Rajagopal R, Beyna M, Chen ZY, Lee FS et al. Cell survival through Trk neurotrophin receptors is differentially regulated by ubiquitination. Neuron 2006; 50: 549–559.