Electron microscopy combined with spatial analysis: quantitative mapping of the nano-assemblies of plasma membrane-associating proteins and lipids

Baumgart T, Hess ST, Webb WW (2003) Imaging coexisting fluid domains in biomembrane models coupling curvature and line tension. Nature 425:821–824

Diggle PJ, Mateu J, Clough HE (2000) A comparison between parametric and non-parametric approaches to the analysis of replicated spatial point patterns. Adv Appl Probab 32:331–343

Hancock JF (2003) Ras proteins: different signals from different locations. Nat Rev Mol Cell Biol 4:373–384

Hancock JF (2006) Lipid rafts: contentious only from simplistic standpoints. Nat Rev Mol Cell Biol 7:456–462

Levental I, Grzybek M, Simons K (2011) Raft domains of variable properties and compositions in plasma membrane vesicles. Proc Natl Acad Sci USA 108:11411–11416

Meyer BH, Segura JM, Martinez KL, Hovius R, George N, Johnsson K, Vogel H (2006) FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells. Proc Natl Acad Sci USA 103:2138–2143

Nan X, Collisson EA, Lewis S, Huang J, Tamguney TM, Liphardt JT, McCormick F, Gray JW, Chu S (2013) Single-molecule superresolution imaging allows quantitative analysis of RAF multimer formation and signaling. Proc Natl Acad Sci USA 110:18519–18524

Plowman SJ, Muncke C, Parton RG, Hancock JF (2005) H-ras, K-ras, and inner plasma membrane raft proteins operate in nanoclusters with differential dependence on the actin cytoskeleton. Proc Natl Acad Sci USA 102:15500–15505

Prior IA, Muncke C, Parton RG, Hancock JF (2003a) Direct visualization of Ras proteins in spatially distinct cell surface microdomains. J Cell Biol 160:165–170

Prior IA, Parton RG, Hancock JF (2003b) Observing cell surface signaling domains using electron microscopy. Sci STKE, PL9

Ripley BD (1977) Modeling spatial patterns. J R Stat Soc Series B Stat Methodol 39:172–192

Simons K, Toomre D (2000) Lipid rafts and signal transduction. Nat Rev Mol Cell Biol 1:31–39

Tian T, Harding A, Inder K, Plowman S, Parton RG, Hancock JF (2007) Plasma membrane nanoswitches generate high-fidelity Ras signal transduction. Nat Cell Biol 9:905–914

Veatch SL, Keller SL (2002) Organization in lipid membranes containing cholesterol. Phys Rev Lett 89:268101

Zhou Y, Hancock JF (2015) Ras nanoclusters: versatile lipid-based signaling platforms. Biochim Biophys Acta 1853:841–849

Zhou Y, Cho KJ, Plowman SJ, Hancock JF (2012) Nonsteroidal anti-inflammatory drugs alter the spatiotemporal organization of Ras proteins on the plasma membrane. J Biol Chem 287:16586–16595

Zhou Y, Liang H, Rodkey T, Ariotti N, Parton RG, Hancock JF (2014) Signal integration by lipid-mediated spatial cross talk between Ras nanoclusters. Mol Cell Biol 34:862–876

Zhou Y, Wong CO, Cho KJ, van der Hoeven D, Liang H, Thakur DP, Luo J, Babic M, Zinsmaier KE, Zhu MX, Hu HZ, Venkalachalam K, Hancock JF (2015) Signal transduction. Membrane potential modulates plasma membrane phospholipid dynamics and K-Ras signaling. Science 349:873–876

Zhou Y, Prakash P, Liang H, Cho KJ, Gorfe AA, Hancock JF (2017) Lipid-sorting specificity encoded in K-Ras membrane anchor regulates signal output. Cell 168(239–251):e216