Improvement in Flavonoids and Phenolic Acids Production and Pharmaceutical Quality of Sweet Basil (Ocimum basilicum L.) by Ultraviolet-B Irradiation

Springer Science and Business Media LLC - Tập 21 Số 9 - Trang 1203
Ali Ghasemzadeh1, Sadegh Ashkani2, Ali Baghdadi1, Alireza Pazoki2, Hawa Z. E. Jaafar1, Asmah Rahmat3
1Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia
2Department of Agronomy and Plant Breeding, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran
3Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

Tóm tắt

Sweet basil (Ocimum basilicum Linnaeus) is aromatic herb that has been utilized in traditional medicine. To improve the phytochemical constituents and pharmaceutical quality of sweet basil leaves, ultraviolet (UV)-B irradiation at different intensities (2.30, 3.60, and 4.80 W/m2) and durations (4, 6, 8, and 10-h) was applied at the post-harvest stage. Total flavonoid content (TFC) and total phenolic content (TPC) were measured using spectrophotometric method, and individual flavonoids and phenolic acids were identified using ultra-high performance liquid chromatography. As a key enzyme for the metabolism of flavonoids, chalcone synthase (CHS) activity, was measured using a CHS assay. Antioxidant activity and antiproliferative activity of extracts against a breast cancer cell line (MCF-7) were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays, respectively. UV-B irradiation at an intensity of 3.60 W/m2 increased TFC approximately 0.85-fold and also increased quercetin (0.41-fold), catechin (0.85-fold), kaempferol (0.65-fold) rutin (0.68-fold) and luteolin (1.00-fold) content. The highest TPC and individual phenolic acid (gallic acid, cinnamic acid and ferulic acid) was observed in the 3.60 W/m2 of UV-B treatment. Cinnamic acid and luteolin were not detected in the control plants, production being induced by UV-B irradiation. Production of these secondary metabolites was also significantly influenced by the duration of UV-B irradiation. Irradiation for 8-h led to higher TFC, TPC and individual flavonoids and phenolic acids than for the other durations (4, 8, and 10-h) except for cinnamic acid, which was detected at higher concentration when irradiated for 6-h. Irradiation for 10-h significantly decreased the secondary metabolite production in sweet basil leaves. CHS activity was induced by UV-B irradiation and highest activity was observed at 3.60 W/m2 of UV-B irradiation. UV-B treated leaves presented the highest DPPH activity and antiproliferative activity with a half-maximal inhibitory concentration (IC50) value of 56.0 and 40.8 µg/mL, respectively, over that of the control plants (78.0 and 58.2 µg/mL, respectively). These observations suggest that post-harvest irradiation with UV-B can be considered a promising technique to improve the healthy–nutritional and pharmaceutical properties of sweet basil leaves.

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Tài liệu tham khảo

2002, Biosynthesis of flavonoids and effects of stress, Curre. Opin. Plant Biol., 5, 218, 10.1016/S1369-5266(02)00256-X

Koes, 2005, Flavonoids: A colorful model for the regulation and evolution of biochemical pathways, Trends Plant Sci., 10, 236, 10.1016/j.tplants.2005.03.002

Dixon, 2002, The phenylpropanoid pathway and plant defence—A genomics perspective, Mol. Plant Pathol., 3, 371, 10.1046/j.1364-3703.2002.00131.x

Ghasemzadeh, 2014, Changes in phytochemical synthesis, chalcone synthase activity and pharmaceutical qualities of Sabah snake grass (Clinacanthus nutans L.) in relation to plant age, Molecules, 19, 17632, 10.3390/molecules191117632

Ghasemzadeh, A., Jaafar, H.Z., Karimi, E., and Ibrahim, M.H. (2012). Combined effect of CO 2 enrichment and foliar application of salicylic acid on the production and antioxidant activities of anthocyanin, flavonoids and isoflavonoids from ginger. BMC Complement. Altern. Med., 12.

Mori, 2005, Decreased anthocyanin biosynthesis in grape berries grown under elevated night temperature condition, Sci. Hortic., 105, 319, 10.1016/j.scienta.2005.01.032

Menezes, 2015, Ultraviolet-B radiation effects on phenolic profile and flavonoid content of Kalanchoe pinnata, J. Photochem. Photobiol. B Biol., 148, 73, 10.1016/j.jphotobiol.2015.03.011

Kneissl, M., and Rass, J. (2016). III-Nitride Ultraviolet Emitters, Springer.

Eichholz, 2012, UV-B-mediated flavonoid synthesis in white asparagus (Asparagus officinalis L.), Food Res. Int., 48, 196, 10.1016/j.foodres.2012.03.008

Schreiner, 2009, Short-term and moderate UV-B radiation effects on secondary plant metabolism in different organs of nasturtium (Tropaeolum majus L.), Innov. Food Sci. Emerg. Technol., 10, 93, 10.1016/j.ifset.2008.10.001

Ries, 2000, Elevated UV-B radiation reduces genome stability in plants, Nature, 406, 98, 10.1038/35017595

Costa, 2002, Effect of UV-B radiation on antioxidant defense system in sunflower cotyledons, Plant Sci., 162, 939, 10.1016/S0168-9452(02)00051-1

Heo, 2009, Effect of phlorotannins isolated from Ecklonia cava on melanogenesis and their protective effect against photo-oxidative stress induced by UV-B radiation, Toxicol. In Vitro, 23, 1123, 10.1016/j.tiv.2009.05.013

Wellmann, 1982, Involvement of phytochrome and a blue light photoreceptor in UV-B induced flavonoid synthesis in parsley (Petroselinum hortense Hoffm.) cell suspension cultures, Planta, 156, 213, 10.1007/BF00393727

Sun, 2010, Change of secondary metabolites in leaves of Ginkgo biloba L. in response to UV-B induction, Innov. Food Sci. Emerg. Technol., 11, 672, 10.1016/j.ifset.2010.08.006

Walter, 2010, Influence of pre-harvest UV-B irradiation and normal or controlled atmosphere storage on flavonoid and hydroxycinnamic acid contents of pak choi (Brassica campestris L. ssp. chinensis var. communis), Postharvest Biol. Technol., 56, 202, 10.1016/j.postharvbio.2010.01.003

Flora of China Ocimum basilicum Linnaeus, Vol.17. Available online: http://efloras.org/florataxon.aspx?flora_id = 2&taxon_id = 200019914.

Jayasinghe, 2003, Phenolics composition and antioxidant activity of sweet basil (Ocimum basilicum L.), J. Agric. Food Chem., 51, 4442, 10.1021/jf034269o

Arranz, 2015, Supercritical fluid extraction as an alternative process to obtain essential oils with anti-inflammatory properties from marjoram and sweet basil, Ind. Crops Prod., 67, 121, 10.1016/j.indcrop.2015.01.012

Kathirvel, 2012, Chemical composition of the essential oil from basil (Ocimum basilicum Linn.) and its in vitro cytotoxicity against HeLa and HEp-2 human cancer cell lines and NIH 3T3 mouse embryonic fibroblasts, Nat. Prod. Res., 26, 1112, 10.1080/14786419.2010.545357

Viyoch, 2006, Evaluation of in vitro antimicrobial activity of Thai basil oils and their micro-emulsion formulas against Propionibacterium acnes, Inte. J. Cosmet. Sci., 28, 125, 10.1111/j.1467-2494.2006.00308.x

Salmah, 2005, Synergistic effects of alcoholic extract of sweet basil (Ocimum basilicum L.) leaves and honey on cutaneous wound healing in rats, Int. J. Mol. Med. Adv. Sci, 1, 220

Marvat, 2011, Phytochemical constituents and pharmacological activities of sweet basil-Ocimum basilicum L. (Lamiaceae), Asian J. Chem., 23, 3773

Jenkins, 2009, Signal transduction in responses to UV-B radiation, Annu. Rev. Plant Biol., 60, 407, 10.1146/annurev.arplant.59.032607.092953

Donno, 2016, Phytochemical fingerprint and chemometrics for natural food preparation pattern recognition: An innovative technique in food supplement quality control, J. Food Sci. Technol., 53, 1071, 10.1007/s13197-015-2115-6

Donno, 2013, Currants and strawberries as bioactive compound sources: Determination of antioxidant profiles with hplc-dad/ms, J. Appl. Bot. Food Qual., 86, 1

Peev, 2007, Determination of some polyphenolic compounds in buds of alnus and corylus species by hplc, Chem. Nat. Compd., 43, 259, 10.1007/s10600-007-0100-7

Olsson, 1998, Differential flavonoid response to enhanced UV-B radiation in Brassica napus, Phytochemistry, 49, 1021, 10.1016/S0031-9422(98)00062-4

Hagen, 2007, Phenolic contents and other health and sensory related properties of apple fruit (Malus domestica Borkh. cv. Aroma): Effect of postharvest UV-B irradiation, Postharvest Biol. Technol., 45, 1, 10.1016/j.postharvbio.2007.02.002

Antognoni, 2007, Induction of flavonoid production by UV-B radiation in Passiflora quadrangularis callus cultures, Fitoterapia, 78, 345, 10.1016/j.fitote.2007.02.001

Rozema, 2002, The role of UV-B radiation in aquatic and terrestrial ecosystems—an experimental and functional analysis of the evolution of UV-absorbing compounds, J. Photochem. Photobiol. B Biol., 66, 2, 10.1016/S1011-1344(01)00269-X

Searles, 2001, A meta-analysis of plant field studies simulating stratospheric ozone depletion, Oecologia, 127, 1, 10.1007/s004420000592

Berli, 2010, Abscisic acid is involved in the response of grape (Vitis vinifera L.) cv. Malbec leaf tissues to ultraviolet-B radiation by enhancing ultraviolet-absorbing compounds, antioxidant enzymes and membrane sterols, Plant Cell Environ., 33, 1

Casati, 2003, Gene expression profiling in response to ultraviolet radiation in maize genotypes with varying flavonoid content, Plant Physiol., 132, 1739, 10.1104/pp.103.022871

Scattino, 2014, Post-harvest UV-B irradiation induces changes of phenol contents and corresponding biosynthetic gene expression in peaches and nectarines, Food Chem., 163, 51, 10.1016/j.foodchem.2014.04.077

Dao, 2011, Chalcone synthase and its functions in plant resistance, Phytochem. Rev., 10, 397, 10.1007/s11101-011-9211-7

Wade, 2001, Interactions within a network of phytochrome, cryptochrome and UV-B phototransduction pathways regulate chalcone synthase gene expression in Arabidopsis leaf tissue, Plant J., 25, 675, 10.1046/j.1365-313x.2001.01001.x

Loyall, 2000, Glutathione and a UV light–induced glutathione S-transferase are involved in signaling to chalcone synthase in cell cultures, Plant Cell, 12, 1939

Park, 2007, Genes up-regulated during red coloration in UV-B irradiated lettuce leaves, Plant Cell Rep., 26, 507, 10.1007/s00299-006-0255-x

Kontogianni, 2013, Phytochemical profile of Rosmarinus officinalis and Salvia officinalis extracts and correlation to their antioxidant and anti-proliferative activity, Food Chem., 136, 120, 10.1016/j.foodchem.2012.07.091

Ghasemzadeh, A., Jaafar, H.Z., Rahmat, A., and Ashkani, S. (2015). Secondary metabolites constituents and antioxidant, anticancer and antibacterial activities of Etlingera elatior (Jack) RM Sm grown in different locations of Malaysia. BMC Complement. Altern. Med., 15.

Ghasemzadeh, 2012, Polyphenolic content and their antioxidant activity in leaf extract of sweet potato (Ipomoea batatas), J. Med. Plants Res., 6, 2971

Ghasemzadeh, A., Talei, D., Jaafar, H.Z., Juraimi, A.S., Mohamed, M.T.M., Puteh, A., and Halim, M.R.A. (2016). Plant-growth regulators alter phytochemical constituents and pharmaceutical quality in Sweet potato (Ipomoea batatas L.). BMC Complement. Altern. Med., 16.

Castagna, 2014, Effect of post-harvest UV-B irradiation on polyphenol profile and antioxidant activity in flesh and peel of tomato fruits, Food Bioprocess Technol., 7, 2241, 10.1007/s11947-013-1214-5

Hansen, 2014, Vitamin C in broccoli (Brassica oleracea L. var. italica) flower buds as affected by postharvest light, UV-B irradiation and temperature, Postharvest Biol. Technol., 98, 82, 10.1016/j.postharvbio.2014.06.017

Palani, 2016, Influence of postharvest UV-B treatment and fermentation on secondary plant compounds in white cabbage leaves, Food Chem., 197, 47, 10.1016/j.foodchem.2015.10.065

Javanmardi, 2003, Antioxidant activity and total phenolic content of Iranian Ocimum accessions, Food Chem., 83, 547, 10.1016/S0308-8146(03)00151-1

2013, Dietary phytochemicals in cancer prevention and therapy: A complementary approach with promising perspectives, Nutr. Rev., 71, 585, 10.1111/nure.12051

Zarlaha, 2014, Cytotoxic activity of essential oil and extracts of Ocimum basilicum against human carcinoma cells. Molecular docking study of isoeugenol as a potent cox and lox inhibitor, J. Nanomater. Biostruct., 9, 907

Dar, 2010, Anticancer activity of Ocimum basilicum and the effect of ursolic acid on the cytoskeleton of MCF-7 human breast cancer cells, Lett. Drug Des. Dis., 7, 726, 10.2174/1570180811007010726

Heim, 2002, Flavonoid antioxidants: Chemistry, metabolism and structure-activity relationships, J. Nutr. Biochem., 13, 572, 10.1016/S0955-2863(02)00208-5