Estimates of bacterial productivity in marine sediments and water from a temperate saltmarsh lagoon
Tóm tắt
Tritiated thymidine incorporation (TTI) into DNA was used to estimate bacterial productivity in sediment and water samples from two sites in Langebaan Lagoon, South Africa. Routine analysis of isotope dilution showed seasonal variations of approximately threefold in the thymidine precursor pool sizes for bacterial assemblages from each site. Dual label incorporation of [3H]-thymidine and 14C-leucine into DNA and protein, respectively, showed that pelagic but not sediment assemblages were in a balanced state of growth during TTI. This is the first report of dual label measurements of bacterial production in sediments. Sediments supported bacterial productivities that exceeded those in the water column by factors from five- to 950-fold, whereas bacterial abundance supported by sediments exceeded that in the water column by more than 3 orders of magnitude. Estimates of bacterial productivities in sediments were coincident with levels of organic content in sediments, but not with bacterial abundance. Measurements of TTI activity for 5 different benthic microhabitats at one lagoon site showed highest activity associated with seagrass beds (2.11 ± 0.84 nmol thymidine hours−1 g-1 dry weight), whereas activities decreased with depth (0.46 ± 0.21 nmol thymidine hours−1 g−I dry weight) below sediment surface.
Tài liệu tham khảo
Alongi DM (1988) Bacterial productivity and microbial biomass in tropical mangrove sediments. Microb Ecol 15:59–79
Branch GM, Pringle A (1987) The impact of the sandprawn Callianassa kraussi stebbing on sediment turnover and on bacteria, meiofauna, and benthic microflora. J Exp Mar Biol Ecol 107:219–235
Brittain AM, Karl DM (1990) Catabolism of tritiated thymidine by aquatic microbial communities and incorporation of tritium into RNA and protein. Appl Environ Microbiol 56: 1245–1254
Chin-Leo G, Kirchman DL (1988) Estimating bacterial production in marine waters from the simultaneous incorporation of thymidine and leucine. Appl Environ Microbiol 54:1934–1939
Chin-Leo G, Kirchman DL (1990) Unbalanced growth in natural assemblages of marine bacterioplankton. Mar Ecol Prog Ser 63:1–8
Davis CL (1989) Uptake and incorporation of thymidine by bacterial isolates from an upwelling environment. Appl Environ Microbiol 55:1267–1272
Ducklow HW, Hill SM, Gardner WD (1985) Bacterial growth and the decomposition of particulate organic carbon collected in sediment traps. Continental Shelf Res 4:445–464
Ellery WN, Schleyer MH (1984) Comparison of homogenization and ultrasonication as techniques in extracting attached sedimentary bacteria. Mar Ecol Prog Ser 15:247–250
Fuhrman JA, Azam F (1980) Bacterioplankton secondary production estimates for coastal waters of British Columbia, Antarctica, and California. Appl Environ Microbiol 39:1085–1095
Fuhrman JA, Azam F (1982) Thymidine incorporation as a measure of heterotrophic bacterio-plankton production in marine surface waters: Evaluation and field results. Mar Biol 66:109–120
Fuhrman JA, Ducklow HW, Kirchman DL, Hudak J, McManus GB, Kramer J (1986) Does adenine incorporation into nucleic acids measure total microbial production? Limnol Oceanogr 31:627–636
Hollibaugh JT (1988) Limitations of the [3H]thymidine method for estimating bacterial productivity due to thymidine metabolism. Mar Ecol Prog Ser 43:19–30
Jeffrey WH, Paul JH (1988) Effect of 5-fluoro-2′-deoxyuridine on [3H]thymidine incorporation by bacterioplankton in the waters of southwest Florida. Appl Environ Microbiol 54:331–336
Jeffrey WH, Paul JH (1990) Thymidine uptake, thymidine incorporation, and thymidine kinase activity in marine bacterium isolates. Appl Environ Microbiol 56:1367–1372
Karl DM (1979) Measurement of microbial activity and growth in the ocean by rates of stable ribonucleic acid synthesis. Appl Environ Microbiol 38:850–860
Karl DM (1982) Selected nucleic acid precursors in studies of aquatic microbial ecology. Appl Environ Microbiol 44:891–902.
Karl DM, Novitsky JA (1988) Dynamics of microbial growth in surface layers of a coastal marine sediment ecosystem. Mar Ecol Prog Ser 50:169–176
Kirchman DL, Hoch MP (1988) Bacterial production in the Delaware Bay estuary estimated from thymidine and leucine incorporated rates. Mar Biol Prog Ser 45:169–178
Lovell CR, Konopka A (1985a) Primary and bacterial production in two dimictic Indiana lakes. Appl Environ Microbiol 49:485–491
Lovell CR, Konopka A (1985b) Seasonal bacterial production in a dimictic lake as measured by increases in cell numbers and thymidine incorporation. Appl Environ Microbiol 49:492–500
Mann KH (1988) Production and use of detritus in various freshwater, estuarine and coastal marine ecosystems. Limnol Oceanogr 33:910–930
Mazure HGF, Branch GM (1979) A preliminary analysis of bacterial numbers and biomass in Langebaan Lagoon. Trans Roy Soc S Afr 44:43–54
Moriarty DJW (1986) Measurement of bacterial growth rates in aquatic systems from rates of nucleic acid synthesis. Adv Microb Ecol 9:245–292
Moriarty DJW, Pollard PC (1981) DNA synthesis as a measure of bacterial productivity in seagrass sediments. Mar Ecol Prog Ser 5:151–156
Moriarty DJW, Pollard PC (1982) Diel variation of bacterial productivity in seagrass (Zostera capricorni) beds measured by rate of thymidine incorporation into DNA. Mar Biol 72:165–173
Moriarty DJW, Pollard PC (1990) Effects of radioactive labeling of macromolecules, disturbance of bacteria and adsorption of thymidine to sediment on the determination of bacterial growth rates in sediment with tritiated thymidine. J Microbiol Methods 11:127–139
Moriarty DJW, Roberts DG, Pollard PC (1990) Primary and bacterial productivity of tropical seagrass communities in the Gulf of Carpentaria, Australia. Mar Ecol Prog Ser 61:145–157
Murray RE, Hodson RE (1985) Annual cycle of bacterial secondary production in five aquatic habitats of the Okefenokee swamp ecosystem. Appl Environ Microbiol 49:650–655
Oremland RS, Capone DG (1988) Use of “specific” inhibitors in biogeochemistry and microbial ecology. Adv Microb Ecol 10:285–383
Phillips RC, McRoy CP (1980) Handbook of seagrass biology-An ecosystem perspective. Garland Press STPM, New York
Pollard PC (1987) Dialysis: A simple method of separating labelled bacterial DNA and tritiated thymidine from aquatic sediments. J Microbiol Methods 7:91–101
Pollard PC, Moriarty DJW (1984) Validity of the tritiated thymidine method for estimating bacterial growth rates: Measurement of isotope dilution during DNA synthesis. Appl Environ Microbiol 48:1076–1083
Porter KG, Feig YS (1980) The use of DAPI for identifying and counting aquatic microflora. Limnol Oceanogr 25:943–948
Riemann B, Bjornsen PK, Newell S, Fallon R (1987) Calculation of cell production of coastal marine bacteria based on measured incorporation of [3H]thymidine. Limnol Oceanogr 32: 471–476
Robarts RD, Wicks RJ (1989) [methyl-3H]thymidine macromoleculer incorporation and lipid labeling: Their significance to DNA labeling during measurements of aquatic bacterial growth rate. Limnol Oceanogr 34:213–222
Robarts RD, Wicks RJ, Sephton LM (1986) Spatial and temporal variations in bacterial macromolecule labeling with [methyl-3H]thymidine in a hypertrophic lake. Appl Environ Microbiol 52:1368–1373
Servais P, Martinez J, Billen G, Vives-Rego J (1987) Determining [3H]thymidine incorporation into bacterioplankton DNA: Improvement of the method by DNase treatment. Appl Environ Microbiol 53:1977–1979
SimonM, Azam F (1989) Protein content and protein synthesis rates of planktonic marine bacteria. Mar Ecol Prog Ser 51:201–213
Thorn PM, Ventullo RM (1988) Measurement of bacterial growth rates in subsurface sediments using the incorporation of tritiated thymidine into DNA. Microb Ecol 16:3–16
Wicks RJ, Robarts RD (1987) The extraction and purification of DNA labeled with [methyl-3H]thymidine in aquatic bacterial production studies. J Plankton Res 9:1159–1166