Distribution and population structure characteristics of microorganisms in urban sewage system

Springer Science and Business Media LLC - Tập 99 - Trang 7723-7734 - 2015
Yanchen Liu1, Qian Dong1, Hanchang Shi1
1State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control, Tsinghua University, Beijing, China

Tóm tắt

The sewage system functions as an important public infrastructure. The survived microbial population inside the sewage system plays an important role in the biochemical process during wastewater transportation within the system. The study aims to investigate the microbial communities spatial distribution inside manholes and sewage pipes by using the massive parallel 454 pyrosequencing combined with denaturing gradient gel electrophoresis of V1–V3 regions of 16S rRNA. The microbial structure, distribution characteristic, taxonomic composition analysis, and compositional overlaps of the microbial community both were conducted. The result indicated that the changes in microbial diversity exhibited a consistent trend with average dehydrogenase activity. Proteobacteria, Firmicutes, and Anaerolineae were the dominant bacteria in the sewage system. The microbial community exhibited distinguishing characteristics in comparison with fecal, surface water, and wastewater treatment process. Parachlamydia acanthamoebae, Zymophilus paucivorans, and uncultured Epsilon proteobacterium were mainly found at the upper position of the manhole, while Microbacterium sp. was mainly found at the lower position. Longilinea, Georgenia, and Desulforhabdus were mainly observed in the sewage pipe. The microbial bacteria that survived in the anaerobic environment (i.e., sulfate reduction bacteria groups) exhibited a significant positive relationship with anaerobic crucial environmental factors in the redundancy analysis.

Tài liệu tham khảo

Bae HS, Moe WM, Yan J, Tiago I, Costa MS, Rainey FA (2006) Brooklawnia cerclae gen. nov., sp. nov., a propionate-forming bacterium isolated from chlorosolvent-contaminated groundwater. Int J Syst Evol Micr 56:1977–1983 Bassit N, Boquien CY, Picque D, Corrieu G (1995) Effect of temperature on diacetyl and acetoin production by Lactococcus lactis subsp. lactis biovar diacetilactis CNRZ 483. J Dary Res 62:123–129 Bernhard AE, Field KG (2000) A PCR assay to discriminate human and ruminant feces on the basis of host differences in Bacteroides-Prevotella genes encoding 16SrRNA. Appl Environ Microbiol 66:4571–4574 Bowers RM, McLetchie S, Knight R, Fierer N (2011) Spatial variability in airborne bacterial communities across land-use types and their relationship to the bacterial communities of potential source environments. Int Soc Microb Ecol 5:601–612 Domingo JWS, Revetta RP, Iker B, Alvarez VG, Garcia J, Sullivan J, Weast J (2011) Molecular survey of concrete sewer biofilm microbial communities. J Bioadhesion and Biofilm 27:993–1001 Dong XL, Reddy GB (2010) Soil bacterial communities in constructed wetlands treated with swine wastewater using PCR-DGGE technique. Bioresource Technol 101:1175–1182 Gihring TM, Green SJ, Schadt CW (2012) Massively parallel rRNA gene sequencing exacerbates the potential for biased community diversity comparisons due to variable library sizes. Environ Microbiol 14:285–290 Han ZS, Tian JY, Liang H, Ma J, Yu HR, Ding A, Li B (2013) Measuring the activity of heterotrophic microorganism in membrane bioreactor for drinking water treatment. Bioresource Technol 130:136–143 Hisashi S, Mitsunori O, Tsukasa I, Satoshi O (2009) Microbial community structures and in situ sulfate reducing and sulfur oxidizing activities in biofilms developed on mortar specimens in a corroded sewer system. Water Res 43:4729–4739 Hugenholtz P, Stackebrandt E (2004) Reclassification of Sphaerobacter thermophilus from the subclass Sphaerobacteridae in the phylum Actinobacteria to the class Thermomicrobia (emended description) in the phylum Chloroflexi (emended description). Int J Syst Evol Microbiol 54:2049–2051 Husen Z, John KD, Andrea Z, Dave K, Michele B, Yeisoo Y, Prathap P, Michael DC, Rod W, Bruce ER, Rosa A (2009) Human gut microbiota in obesity and after gastric bypass. Proceedings of the National Academy of Sciences of the United States of America. PNAS 106:2365–2370 Jiang G, Oriol G, Keshab RS, Zhiguo Y (2010) Effects of nitrite concentration and exposure time on sulfide and methane production in sewer systems. Water Res 44:4241–4251 Jiang F, Zhang L, Peng GL, Liang SY, Qian J, Wei L, Chen GH (2013) A novel approach to realize SANI process in freshwater sewage treatmente use of wet flue gas desulfurization waste streams as sulfur source. Water Res 47:5773–5782 John DM, White GF (1998) Mechanism for Biotransformation of Nonylphenol Polyethoxylates to Xenoestrogens in Pseudomonas putida. J Bacteriol 180:4332–4338 John TW, Eunji K, John AB, Thomas MS, Jorge LMR (2011) Genomic and Physiological Characterization of the Verrucomicrobia Isolate Diplosphaera colitermitum gen. nov., sp. nov., Reveals Microaerophily and Nitrogen Fixation Genes. Appl Environ Microbiol 78:1554–1555 Karpf C, Krebs P (2011) Quantification of groundwater infiltration and surface water inflows in urban sewer networks based on a multiple model approach. Water Res 45:3129–3136 Lettinga G, Rebac S, Zeeman G (2001) Challenge of psychrophilic anaerobic wastewater treatment. Trends Biotechnol 19:363–370 Lim YW, Lee SA, Kim SB, Yong HY, Yeon SH, Park YY, Jeong DW, Park JS (2005) Diversity of denitrifying bacteria isolated from Daejeon sewage treatment plant. J Microbiol 43:283–290 Liu XC, Zhang Y, Yang M, Wang ZY, Lv WZ (2007) Analysis of bacterial community structures in two sewage treatment plants with different sludge properties and treatment performance by nested PCR-DGGE method. J Environ Sci 19:60–66 Liu J, Lu ZB, Zhang J, Xing MY, Yang J (2013) Phylogenetic characterization of microbial communities in a full-scale vermifilter treating rural domestic sewage. Ecol Eng 61:100–109 Liu Y, Dong Q, Wu C, Zhou X, Shi H (2015a) Study of the succession of microbial communities for sulfur cycle response to ecological factors change in sediment of sewage system. Envir Sci Pollut Res. doi:10.1007/s11356-014-3934-0 Liu YC, Wu C, Zhou XH, Zhang TJ, Mu L, Shi HC (2015b) Effect of variation of liquid condition on transformation of sulfur and carbon in the sediment of sanitary sewer. J Environ Manag 154:65–69 Marques JM, Almeida FP, Lins U, Seldin L, Korenblum E (2012) Nitrate treatment effects on bacterial community biofilm formed on carbon steel in produced water stirred tank bioreactor. World J Microbl Biot 28:2355–2363 Matsusaki H, Endo N, Sonomoto K, Ishizaki A (1996) Lantibiotic nisin Z fermentative production by Lactococcus lactis IO-1: relationship between production of the lantibiotic and lactate and cell growth. Appl Microbiol Biotechnol 45:36–40 McLellan SL, Huse SM, Mueller-Spitz SR, Andreishcheva EN, Sogin ML (2010) Diversity and population structure of sewage-derived microorganisms in wastewater treatment plant influent. Environ Microbiol 12:378–392 Mohanakrishnan J, Gutierrez O, Meyer RL, Yuan Z (2008) Nitrite effectively inhibits sulfide and methane production in a laboratory scale sewer reactor. Water Res 42:3961–3971 Newton RJ, McLellan SL, Dila DK, Vineis JH, Morrison HG, Eren AM, Sogin ML (2015) Sewage reflects the microbiomes of human populations. mBio 6 : e02574-14 Nielsen PH, Raunkjaer K, Hvitved-Jacobsen TH (1992) Transformation of wastewater in sewer system- a review. Water Sci Technol 25:17–31 Ogunseitan OA (1996) Removal of caffeine in sewage by Pseudomonas putida: implications for water pollution index. World J Microbl Biot 12:251–256 Okabe S, Odagiri M, Ito T, Satoh H (2007) Succession of sulfur-oxidizing bacteria in the microbial community on corroding concrete in sewer systems. Appl Environ Microbiol 73:971–980 Pai TY, Chen CL, Chung H, Ho HH, Shiu TW (2010) Monitoring and assessing variation of sewage quality and microbial functional groups in a trunk sewer line. Environ Monit Assess 171:551–560 Satoh H, Odagiri M, Ito T, Okabe S (2009) Microbial community structures and in situ sulfate-reducing and sulfur-oxidizing activities in biofilms developed on mortar specimens in a corroded sewer system. Water Res 43:4729–4739 Soleimani S, Ormeci B, Isgor B (2013) Growth and characterization of Escherichia coli DH5α biofilm on concrete surfaces as a protective layer against microbiologically influenced concrete deterioration (MICD). Appl Microbiol Biot 97:1093–1102 Taketani RG, Yoshiura CA, Dias ACF, Andreote FD, Tsai SM (2010) Diversity and identification of methanogenic archaea and sulphate-reducing bacteria in sediments from a pristine tropical mangrove. Anton Leeuw Int J G 97:401–411 Ternes TA, Joss A, Siegrist H (2004) Scrutinizing pharmaceuticals and personal care products in wastewater treatment. Environ Sci Technol 38:392–399 USEPA (2004) Report to congress: impacts and control of CSOs and SSOs. US EPA Office of Water, Washington, DC, USA VandeWalle JL, Goetz GW, Huse SM, Morrison HG, Sogin ML, Hoffmann RG, Yan K, McLellan SL (2012) Acinetobacter, Aeromonas and Trichococcus populations dominate the microbial community within urban sewer infrastructure. Environ Microbiol 14:2538–2552 Vincke E, Boon W, Verstraete W (2001) Analysis of the microbial communities on corroded concrete sewer pipes – a case study. Appl Microbiol Biot 57:776–785 Vishnivetskaya TA, Mosher JJ, Palumbo AV, Yang ZK, Podar M, Brown SD, Brooks SC, Gu B, Southworth GR, Drake MM, Brandt CC, Elias DA (2011) Mercury and other heavy metals influence bacterial community structure in contaminated tennessee streams. Appl Environ Microb 77:302–311 Yongsiri C, Hvitved-Jacobsen T, Vollertsen J, Tanaka N (2003) Introducing the emission process of hydrogen sulfide to a sewer process model (WATS). Water Sci Technol 47:85–92 Yoon DN, Park SJ, Kim SJ, Jeon CO, Chae JC, Rhee SK (2010) Isolation, characterization, and abundance of filamentous members of Caldilineae in activated sludge. J Microbiol 48:275–283 Zhang L, Peter DS, Bart DG, Willem DM, Nico B, Willy V (2008) Chemical and biological technologies for hydrogen sulfide emission control in sewer systems: a review. Water Res 42:1–12