Monitoring sãto paulo state rivers in brazil for mutagenic activity using the ames test

Wiley - Tập 8 Số 4 - Trang 371-381 - 1993
Gisela de Aragão Umbuzeiro1, Maria Inês Zanoli Sato1, Maria Luisa Cristina1, Letícia Coelho1, Carlos Alberto Coimbrão1, P. S. Sanchez1, Maria Therezinha Martins2, Renato Bonatelli3
1Microbiology Division, Companhia de Tecnologia de Saneamento Ambiental—CETESB, Av. Prof. Frederico Hermann, Jr., 345, 05489–900–São Paulo, SP‐Brasil
2Department of Microbiology, ICB, Universidade de São Paulo—USP, São Paulo—Brasil
3Department of Genetics and Evolution, Universidade Estadual de Campinas—UNICAMP, Campinas, SP,—Brasil

Tóm tắt

AbstractOrganic extracts of raw water from 11 water courses of São Paulo State, Brazil, were collected during one year bimonthly and tested for mutagenicity using the Ames test, with strains TA98 and TA100 of Salmonella typhimurium with and without metabolic activation. The samples were extracted with XAD2 resin and eluted with methanol and methylene chloride. From the 75 samples analyzed, 14 showed positive responses and 8 were considered marginal, making up 29% of mutagenic samples. The percentage of mutagenic samples in October (spring) was 9%, increasing to 64% in February (summer), and decreased to 9% again in August (winter). Paraiba do Sul river showed the higher percentage of mutagenic samples (67%) and Capivari river the highest mutagenic sample (1787 and 3265 revertants per liter for TA98 without and with S9, respectively).The amplitude of the mutagenic response was 39–3265 revertants per liter for TA98 and 83–467 for TA100. The mutagenic samples showed direct and indirect mutagens, and TA98 detected the majority of responses, indicating prevalence of frameshift mutagens in these samples. © 1993 John Wiley & Sons, Inc.

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Wiley

Tập 8 Số 4

371-381

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