Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products

Saiki, R.K. et al. Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230, 1350–1354 (1985).

Higuchi, R., Dollinger, G., Walsh, P.S. & Griffith, R. Simultaneous amplification and detection of specific DNA sequences. Biotechnology (NY) 10, 413–417 (1992).

Higuchi, R., Fockler, C., Dollinger, G. & Watson, R. Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Biotechnology (NY) 11, 1026–1030 (1993).

Holland, P.M., Abramson, R.D., Watson, R. & Gelfand, D.H. Detection of specific polymerase chain reaction product by utilizing the 5′→3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88, 7276–7280 (1991).

Tyagi, S. & Kramer, F.R. Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. 14, 303–308 (1996).

Whitcombe, D., Theaker, J., Guy, S.P., Brown, T. & Little, S. Detection of PCR products using self-probing amplicons and fluorescence. Nat. Biotechnol. 17, 804–807 (1999).

Zhang, D.Y., Brandwein, M., Hsuih, T.C. & Li, H. Amplification of target-specific, ligation-dependent circular probe. Gene 211, 277–285 (1998).

Compton, J. Nucleic acid sequence-based amplification. Nature 350, 91–92 (1991).

Guatelli, J.C. et al. Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication. Proc. Natl. Acad. Sci. USA 87, 1874–1878 (1990).

Walker, G.T. et al. Strand displacement amplification—an isothermal, in vitro DNA amplification technique. Nucleic Acids Res. 20, 1691–1696 (1992).

Notomi, T. et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28, e63 (2000).

Fukuta, S. et al. Detection of Japanese yam mosaic virus by RT-LAMP. Archives of Virology 148, 1713–1720 (2003).

Mori, Y., Nagamine, K., Tomita, N. & Notomi, T. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem. Biophys. Res. Commun. 289, 150–154 (2001).

Diehl, H. & Ellingboe, J.L. Indicator for titration of calcium in presence of magnesium using disodium dihydrogen ethylenediamine tetraacetate. Anal. Chem. 28, 882–884 (1956).

Kepner, B.L. & Hercules, D.M. Fluorometric determination of calcium in blood serum. Anal. Chem. 35, 1238–1240 (1963).

Hoelzl Wallach, D.F. & Steck, T.L. Fluorescence techniques in the microdetermination of metals in biological materials. Utility of 2, 4-bis-(N,N′-di-(carboxymethyl)aminomethyl) fluorescein in the fluorometric estimation of Al+3, alkaline earths, Co+2, Cu+2, Ni+2, and Zn+2 in micromolar concentrations. Anal. Chem. 35, 1035–1044 (1963).

Körbl, J. & Vydra, F. Metallochromic indicators. IV. A note on the preparation and properties of “Calcein”. Collect. Czech. Chem. Commun. 23, 622–627 (1958).

Demertzis, M.A. Fluorimetric determination of calcium in serum with calcein: complexation of calcein with calcium and alkali metals. Anal. Chim. Acta. 209, 303–308 (1988).

Nagamine, K., Watanabe, K., Ohtsuka, K., Hase, T. & Notomi, T. Loop-mediated isothermal amplification reaction using a nondenatured template. Clin. Chem. 47, 1742–1743 (2001).